HBV包膜蛋白N-糖基化修饰变异与HBV相关慢加急性肝衰竭发生和转归的关系

陈杰; 姚明琦; 魏飞力; 徐金凤; 郭乐乐; 杨海霞; 李铭; 张晶

doi:10.3969/j.issn.1001-5256.2018.07.013

HBV包膜蛋白N-糖基化修饰变异与HBV相关慢加急性肝衰竭发生和转归的关系

DOI: 10.3969/j.issn.1001-5256.2018.07.013

1. 首都医科大学附属北京佑安医院丙肝和中毒肝病科
2. 山西省运城市第二医院肝病二科
3. 北京市肝炎研究所
4. 安徽省阜阳市第二人民医院

基金项目:

艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2018ZX10715005-003-003)；

详细信息

中图分类号: R512.62;R575.3
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出版历程
- 收稿日期: 2018-01-11
- 出版日期: 2018-07-20

Association of N-glycosylation mutation of HBV envelope protein with the development and prognosis of acute-on-chronic liver failure in patients with chronic hepatitis B

Department of Hepatitis C and Hepatology, Beijing You An Hospital, Capital Medical University

Research funding:

摘要

摘要: 目的探索HBV包膜蛋白N-糖基化修饰变异与慢性乙型肝炎患者发生慢加急性肝衰竭（ACLF）的关系。方法入选HBV-ACLF患者90例,同期60例慢性乙型肝炎作对照。套式PCR扩增患者的HBV S区序列并测序,分析HBV包膜蛋白N-糖基化情况与ACLF发生的关系,并分析ACLF疾病严重程度、90 d生存率与HBV包膜蛋白N-糖基化的关系。结果慢性乙型肝炎患者纳入分析51例（85.0%）,HBV-ACLF患者纳入分析79例（87.8%）。慢性乙型肝炎患者Asn59 N-糖基化样本33例（64.7%）,Asn4 N-糖基化样本21例（41.2%）,高于HBV-ACLF患者的Asn59 N-糖基化样本16例（20.3%）（P<0.001）和Asn4 N-糖基化样本14例（17.7%）（P=0.003）。ACLF患者中Asn59、Asn4 N-糖基化与非糖基化患者肝功能水平、HBV DNA、MELD评分及90 d生存率差异均无统计学意义（P值均>0.05）。结论 HBV包膜蛋白变异导致的Asn59、Asn4 N-糖基化水平降低与ACLF发生可能相关。
- 肝炎病毒,乙型 /
- 肝功能衰竭 /
- 病毒包膜蛋白类 /
- 糖基化
Abstract: Objective To investigate the association of N-glycosylation mutation of HBV envelope protein with the development of acute-on-chronic liver failure ( ACLF) in patients with chronic hepatitis B ( CHB) . Methods A total of 90 patients with HBV-ACLF were enrolled, and 60 patients with CHB were enrolled as control group. Nested PCR was used for the multiplication and sequencing of HBV S region, and the association of N-glycosylation of HBV envelope protein with the development ACLF was analyzed, as well as the association of N-glycosylation of HBV envelope protein with the severity and 90-day survival rate of ACLF. Results A total of 51 CHB patients ( 85. 0%) and 79 HBV-ACLF patients ( 87. 8%) were included in analysis. Among the CHB patients, 33 ( 64. 7%) had Asn-59 N-glycosylation and 21 ( 41. 2%) had Asn-4 N-glycosylation, while among the HBV-ACLF patients, 16 ( 20. 3%) had Asn-59 N-glycosylation and 14 ( 17. 7%) had Asn-4 N-glycosylation; there were significant differences in the numbers of cases of Asn-59 and Asn-4 N-glycosylation between the two groups ( P < 0. 001, and P = 0. 003) . There were no significant differences in liver function, HBV DNA, Model for End-Stage Liver Disease score, and 90-day survival rate between the ACLF patients with Asn-59 and Asn-4 N-glycosylation and those without such glycosylation ( P > 0. 05) . Conclusion The reduction in Asn-59 and Asn-4 N-glycosylation induced by HBV envelope protein variation may be associated with the development of ACLF.
- hepatitis B virus /
- liver failure /
- viral envelope proteins /
- glycosylation

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参考文献(16)

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