Tumor-stroma crosstalk对肝细胞癌中肝星状细胞氨基酸代谢水平的影响

吴静; 孟庆华; 薛冉

doi:10.3969/j.issn.1001-5256.2018.12.020

Tumor-stroma crosstalk对肝细胞癌中肝星状细胞氨基酸代谢水平的影响

DOI: 10.3969/j.issn.1001-5256.2018.12.020

1. 首都医科大学附属北京佑安医院肝病重症医学科
2. 首都医科大学附属北京朝阳医院消化内科

基金项目:

首都卫生发展科研专项(2016-1-2181)；

详细信息

中图分类号: R735.7
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出版历程
- 出版日期: 2018-12-20

Effect of tumor-stroma crosstalk on amino acid metabolism in hepatic stellate cells

Research funding:

摘要

摘要: 目的探讨tumor-stroma crosstalk对肝星状细胞（HSC）氨基酸代谢的影响。方法分别培养人肝癌细胞系HepG2、Hep3B、Huh7,以及LX-2 HSC。分别使用LX-2 HSC条件培养基（LX2-CM）和HepG2、Hep3B、Huh7肝癌细胞混合条件培养基（Hep-CM）培养HSC,并收集细胞上清,应用氨基酸分析仪检测细胞上清氨基酸谱变化。计量资料组间比较采用t检验。结果HSC氨基酸代谢水平改变情况,与对照组（LX2-CM）相比,实验组（Hep-CM）上清中瓜氨酸（t=3. 426,P=0. 027）、缬氨酸（t=2. 892,P=0. 045）、异亮氨酸（t=4. 224,P=0. 013）、亮氨酸（t=4. 150,P=0. 014）、酪氨酸（t=3. 556,P=0. 024）、苯丙氨酸（t=4. 023,P=0. 016）、赖氨酸（t=3. 369,P=0. 028）水平降低,差异均有统计学意义。结论肿瘤微环境中,tumor-stroma crosstalk可以影响HSC的氨基酸代谢水平,这种改变可能反过来促使肝癌细胞更加适应低氧微环境。
- 肝肿瘤 /
- 氨基酸类 /
- 代谢 /
- tumor-stroma crosstalk
Abstract: Objective To investigate the effect of tumor-stroma crosstalk on amino acid metabolism in hepatic stellate cells (HSCs) .Methods Human hepatoma cell lines HepG2, Hep3 B, and Huh7 and the HSC LX-2 were cultured separately. HSCs were cultured in HSC LX-2 conditioned medium (LX2-CM) and HepG2/Hep3 B/Huh7 mixed conditioned medium (Hep-CM) , respectively. The supernatants were collected and an amino acid analyzer was used to measure the change in aminogram. The t-test was used for comparison of continuous data between groups. Results There was a significant change in amino acid metabolism in HSCs. Compared with the LX2-CM control group, the Hep-CM experimental group had significant reductions in the levels of citrulline (t = 3. 426, P = 0. 027) , valine (t = 2.892, P = 0. 045) , isoleucine (t = 4. 224, P = 0. 013) , leucine (t = 4. 150, P = 0. 014) , tyrosine (t = 3. 556, P = 0. 024) , phenylalanine (t = 4. 023, P = 0. 016) , and lysine (t = 3. 369, P = 0. 028) in cell supernatant. Conclusion Tumor-stroma crosstalk can influence amino acid metabolism in HSCs in tumor microenvironment, and such change in turn makes hepatoma cells better adapted to hypoxic microenvironment.
- liver neoplasms /
- amino acids /
- metabolism /
- tumor-stroma crosstalk

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参考文献(17)

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