中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

清温并用法对慢加急性肝衰竭大鼠调节性T淋巴细胞/辅助性T淋巴细胞17表达的影响

陈斌 张涛 朱文芳 彭杰 张茜茜 黎运芳 丁秀丽

陈斌, 张涛, 朱文芳, 彭杰, 张茜茜, 黎运芳, 丁秀丽. 清温并用法对慢加急性肝衰竭大鼠调节性T淋巴细胞/辅助性T淋巴细胞17表达的影响[J]. 临床肝胆病杂志, 2018, 34(12): 2642-2647. DOI: 10.3969/j.issn.1001-5256.2018.12.026.
引用本文: 陈斌, 张涛, 朱文芳, 彭杰, 张茜茜, 黎运芳, 丁秀丽. 清温并用法对慢加急性肝衰竭大鼠调节性T淋巴细胞/辅助性T淋巴细胞17表达的影响[J]. 临床肝胆病杂志, 2018, 34(12): 2642-2647. DOI: 10.3969/j.issn.1001-5256.2018.12.026.
Chen Bin, Zhang Tao, Zhu WenFang, Peng Jie, Zhang QianQian, Li YunFang, Ding XiuLi. Effect of the warming and heat-clearing method on the percentage of regulatory T lymphocytes/T helper 17 cells in rats with acute-on-chronic liver failure[J]. J Clin Hepatol, 2018, 34(12): 2642-2647. DOI: 10.3969/j.issn.1001-5256.2018.12.026.
Citation: Chen Bin, Zhang Tao, Zhu WenFang, Peng Jie, Zhang QianQian, Li YunFang, Ding XiuLi. Effect of the warming and heat-clearing method on the percentage of regulatory T lymphocytes/T helper 17 cells in rats with acute-on-chronic liver failure[J]. J Clin Hepatol, 2018, 34(12): 2642-2647. DOI: 10.3969/j.issn.1001-5256.2018.12.026.

清温并用法对慢加急性肝衰竭大鼠调节性T淋巴细胞/辅助性T淋巴细胞17表达的影响

DOI: 10.3969/j.issn.1001-5256.2018.12.026
基金项目: 

国家自然科学基金(81673959); 

详细信息
  • 中图分类号: R575.3

Effect of the warming and heat-clearing method on the percentage of regulatory T lymphocytes/T helper 17 cells in rats with acute-on-chronic liver failure

Research funding: 

 

  • 摘要:

    目的观察清温并用法对慢加急性肝衰竭(ACLF)大鼠调节性T淋巴细胞(Treg)/辅助性T淋巴细胞(Th) 17表达的影响,探索其抗肝衰竭的作用机制。方法 SD大鼠140只随机分为正常组、模型组、清法组、温法组及清温并用法组。采用牛血清白蛋白致敏建立免疫性肝纤维化大鼠模型,然后以D-氨基半乳糖+脂多糖攻击建立ACLF模型。观察24 h内各组大鼠血清肝功能(ALT、TBil)、肝组织病理学改变及外周血Treg、Th17细胞数,并计算Treg/Th17比值。计量资料多组间比较釆用One-Way ANOVA比较,进一步两两比较选用LSD-t法检验。结果与正常组比较,模型组大鼠ALT、TBil在各时间点明显升高(P值均<0. 01),肝小叶结构被破坏,肝细胞见桥接及片状坏死,汇管区可见炎细胞浸润,外周血Treg、Th17细胞比例明显升高(P <0. 01),且Th17高于Treg,Treg/Th17比值明显降低(P <0. 01)。与模型组比较,清法组、温法组、清温并用法组ALT、TBil明显降低(P <0. 05),肝小叶结构较完整,肝细胞可见小片状、点状坏死,门区少量...

     

  • [1] Liver Failure and Artificial Liver Group, Chinese Society of Infectious Diseases, Chinese Medical Association; Severe Liver Diseases and Artificial Liver Group, Chinese Society of Hepatology, Chinese Medical Association. Guidelines for diagnosis and treatment liver failure[J]. Chin J Clin Infect Dis, 2012, 5 (6) :321-327. (in Chinese) 中华医学会感染病学分会肝衰竭与人工肝学组, 中华医学会肝病学分会重型肝病与人工肝学组.肝衰竭诊治指南 (2012年版) [J].中华临床感染病杂志, 2012, 5 (6) :321-327.
    [2] RICHARD M, VICENTE A. Acute-on-chronic liver failure:A new clinical entity[J]. Clin Gastroenterol Hepatol, 2015, 13 (5) :836-841.
    [3] DENG H. Correlation between Treg/Th17 imbalance and the liver injury caused by systemic inflammatory response and oxidative stress in children with sepsis[J]. J Hanan Med Coll, 2017, 23 (21) :2968-2971. (in Chinese) 邓辉.脓毒症患儿Treg/Th17失衡与全身炎症反应、氧化应激所致肝损伤的相关性[J].海南医学院学报, 2017, 23 (21) :2968-2971.
    [4] CHEN B, WANG J, PENG J, et al. Effect of Wen-Yang Jie-Du Hua-Yu granules on TLR4 and NF-κB expression of rat hepatic failure model with intestinal endotoxemia[J]. World Sci Technol Modern Tradit Chin Med, 2013, 15 (5) :818-824. (in Chinese) 陈斌, 王杰, 彭杰, 等.温阳解毒化瘀颗粒对肝衰竭肠源性内毒素血症大鼠TLR4、NF-κB表达的影响[J].世界科学技术-中医药现代化, 2013, 15 (5) :818-824.
    [5] ZHU WF, SUN KW, CHEN B, et al. Effect of Wenyang Jiedu Huayu prescription on intestinal bacteria in patients with HBV related liver failure[J]. Chin J Integr Tradit West Med Liver Dis, 2014, 24 (4) :214-216. (in Chinese) 朱文芳, 孙克伟, 陈斌, 等.温阳解毒化瘀方对HBV相关肝衰竭患者肠道菌群的影响[J].中西医结合肝病杂志, 2014, 24 (4) :214-216.
    [6] ZHU QG, FANG BW, ZHU JN, et al. Animal models of bovine serum albumin-induced immune liver fibrosis[J]. Chin Jf Integr Tradition Western Med Liver Dis, 1992, 2 (1) :20-24. (in Chinese) 朱起贵, 方步武, 竺稽能, 等.牛血清白蛋白免疫性肝纤维化动物模型的研究[J].中西结合肝病杂志, 1992, 2 (1) :20-24.
    [7] LIU XH, CHEN Y, ZHANG J, et al. Human serum albumin and a rat's model for d-galactosamine/lipopolysacchar ide induced acute-on-chronic liver failure[J]. Sci Technol Rev, 2007, 25 (18) :32-37. (in Chinese) 刘旭华, 陈煜, 张晶, 等.大鼠慢加急性肝衰竭实验模型的建立及其病理机制初步研究[J].科技导报, 2007, 25 (18) :32-37.
    [8] XU Y, LIN H, ZHENG W, et al. Matrine ameliorates adriamycin-induced nephropathy in rats by enhancing renal function and modulating Th17/Treg balance[J]. Eur J Pharmacol, 2016, 15 (7) :491-501.
    [9] YANG C, CUI F, CHEN LM, et al. Correlation between Th17 and nTreg cell frequencies and the stages of progression in chronic hepatitis B[J]. Mol Med Rep, 2016, 13 (1) :853-862.
    [10] MING M, LUO Z, LV S, et al. Inactivated Mycobacterium phlei inhalation ameliorates allergic asthma through modulating the balance of CD4+CD25+regulatory T and Th17 cells in mice[J]. Iran J Basic Med Sci, 2016, 19 (9) :953-959.
    [11] SHI WJ, JIA JT, LI CD, et al. Expression and significance of Th17/Treg cytokines in patients with hepatitis B-related acute-on-chronic liver failure[J]. Chin Hepatol, 2016, 21 (2) :92-94. (in Chinese) 施文娟, 贾瑾堂, 李彩东, 等. Th17/Treg细胞因子在乙型肝炎慢加急性肝衰竭患者中的表达及意义[J].肝脏, 2016, 21 (2) :92-94.
    [12] GAO HB, WANG XM, LIU BR, et al. Features of regulatory T cells and T helper 17 cells and their association with pre-C/BCP mutations in HBV in patients with HBV-related acute-on-chronic liver failure[J]. Chin Hepatol, 2018, 23 (1) :18-21. (in Chinese) 高海兵, 王香梅, 刘宝荣, 等. HBV相关慢加急性肝衰竭患者Treg细胞、Th17细胞表达特点及其与HBV前C区/BCP区变异的相关性[J].肝脏, 2018, 23 (1) :18-21.
    [13] ZHU L, XI Y, ZHAO L, et al. Intervenient study of Shengjiang San on Th17/Treg imbalances and related regulatory factors in sepsis patients[J]. J Clin Emerg, 2017, 18 (6) :406-409. (in Chinese) 朱亮, 奚耀, 赵雷, 等.升降散对脓毒症患者Th17/Treg失衡及相关调节因子的干预作用[J].临床急诊杂志, 2017, 18 (6) :406-409.
    [14] JIA L, WU J, QIU ZJ, et al. Effects of Yupingfeng Powder on balance of Th17/Treg in allergic rhinitis mice[J]. China J Tradit Chin Med Pharma, 2016, 31 (8) :3260-3262. (in Chinese) 贾翎, 吴珺, 邱泽计, 等.玉屏风散对过敏性鼻炎小鼠Th17/Treg平衡的影响[J].中华中医药杂志, 2016, 31 (8) :3260-3262.
    [15] WU LM, ZHAO JF. Role of Treg and Th17 cells in the pathogenesis of hepatitis B-induced acute-on-chronic liver failure[J].J Pract Hepatol, 2017, 20 (5) :639-642. (in Chinese) 吴丽蒙, 赵久法. Treg和Th17细胞在慢加急性乙型肝炎肝衰竭发病机制中的作用研究进展[J].实用肝脏病杂志, 2017, 20 (5) :639-642.
    [16] KAN YT, GAN JH, SUN W, et al. Changes in regulatory T cells and T helper 17 cells in patients with HBV-related acute-onchronic liver failure and related clinical significance[J]. Chin Hepatol, 2016, 21 (2) :89-91. (in Chinese) 阚延婷, 甘建和, 孙蔚, 等. HBV相关慢加急性肝衰竭患者Treg、Th17变化及其与临床相关性[J].肝脏, 2016, 21 (2) :89-91.
    [17] YU SJ, JIANG R, MAZZU YZ, et al. Epigallocatechin-3-gallate prevents triptolide-induced hepatic injury by restoring the Th17/Treg balance in mice[J]. Am J Chin Med, 2016, 44 (6) :1221-1236.
    [18] XUE S, CHEN Z, YIN Z, et al. Changes in circulating Foxp3+regulatory T cells and interleukin-17-producing T helper cells during HBV-related acute-on-chronic liver failure[J]. World J Gastroenterol, 2014, 20 (26) :8558-8571.
    [19] MICROAL B. Systemic inflammation, and multiorgan dysfunction in decompensated cirrhosis:Evidence for a nonfunctional mechanism of hepatorenal syndrome[J]. Hepatology, 2017, 11 (3) :242-244.
  • 期刊类型引用(10)

    1. 李晏杰,王娜,王沙,唐鲲,毛德文,石清兰,龙富立. HBV相关性肝衰竭发病中的Th细胞免疫调控机制及中医药干预的研究进展. 内蒙古中医药. 2023(11): 134-138 . 百度学术
    2. 霍瑞静,张翠丽,李静,何晨熙,李帅,高健. NF-κB信号通路对慢性乙型肝炎患者Th17/Treg相关细胞因子水平变化的影响. 中华医院感染学杂志. 2022(17): 2570-2573 . 百度学术
    3. 黄杏,刘文志,陈斌. 清温并用法治疗肝衰竭的中医理论及作用机制探讨. 中国医学创新. 2021(10): 164-167 . 百度学术
    4. 陈斌,丁秀丽,彭杰,黎运芳,王若宇,周意. 清温并用法对慢加急性肝衰竭肠源性内毒素血症大鼠结肠组织FoxP3, ROR-γt表达的影响. 中国实验方剂学杂志. 2020(02): 19-25 . 百度学术
    5. 谭年花,陈斌,彭杰,朱文芳,张涛,周意,王若宇. 乙肝相关性慢加急性肝衰竭“阳黄-阴阳黄-阴黄”不同证候分型患者Treg/Th17的表达. 中国实验方剂学杂志. 2020(02): 33-38 . 百度学术
    6. 陈斌,黎运芳,张涛,朱文芳,彭杰,张茜茜. 清温并用法对慢加急性肝衰竭大鼠肝组织Treg/Th17型细胞因子及转录因子的影响. 中华中医药杂志. 2020(09): 4618-4622 . 百度学术
    7. 李薇,于佳卉,王新婷,王春梅,李贺,陈建光,孙靖辉. 北五味子总木脂素对环磷酰胺诱导的免疫功能低下小鼠的调节作用. 吉林大学学报(医学版). 2019(02): 294-299+472 . 百度学术
    8. 陈斌,彭杰,张涛,朱文芳,张茜茜,丁秀丽,黎运芳. 清温并用法对慢加急性肝衰竭大鼠结肠组织调节性T淋巴细胞/辅助性T淋巴细胞17细胞因子的影响. 湖南中医药大学学报. 2019(05): 573-577 . 百度学术
    9. 张龙跃,张序瑞. 赤芍解毒汤保留灌肠治疗慢加急性肝衰竭临床研究. 系统医学. 2019(13): 138-140 . 百度学术
    10. 梁琼,赵隽,苏静,梁琳,张吉发. 凉血化瘀解毒法对急性肝衰竭模型大鼠免疫功能、Toll样受体及VEGF表达的影响. 中国中医急症. 2019(10): 1700-1704 . 百度学术

    其他类型引用(5)

  • 加载中
计量
  • 文章访问数:  1826
  • HTML全文浏览量:  35
  • PDF下载量:  298
  • 被引次数: 15
出版历程
  • 收稿日期:  2018-06-29
  • 出版日期:  2018-12-20
  • 分享
  • 用微信扫码二维码

    分享至好友和朋友圈

目录

    /

    返回文章
    返回