干扰素λ3在慢性乙型肝炎患者血清中的表达及临床意义

段元丽; 管世鹤; 杨凯; 陈礼文; 张浩; 王秀; 潘正兰; 侯舒文; 王兆飞

doi:10.3969/j.issn.1001-5256.2019.02.014

干扰素λ3在慢性乙型肝炎患者血清中的表达及临床意义

DOI: 10.3969/j.issn.1001-5256.2019.02.014

安徽医科大学第二附属医院检验科

基金项目:

安徽省科技厅重点研究与开发计划项目(1804h08020236)；安徽省高校自然科学研究项目(KJ2018A0206)；

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中图分类号: R512.62
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出版历程
- 出版日期: 2019-02-20

Serum level of interferon-λ3 in patients with chronic hepatitis B and its clinical significance

Department of Clinical Laboratory, The Second Affiliated Hospital of Anhui Medical University

Research funding:

摘要

摘要:
目的了解慢性乙型肝炎患者血清IFNλ3水平,探究其临床意义及其与核苷和核苷酸类药物治疗的关系。方法选取2017年1月-2018年5月在安徽医科大学第二附属医院就诊的慢性乙型肝炎患者119例,收集同期HBsAg阴性的22例健康体检者作为健康对照。采用ELISA法检测血清中IFNλ3的表达水平,比较慢性乙型肝炎患者与健康人血清IFNλ3水平差异,分析慢性乙型肝炎患者血清IFNλ3水平与HBeAg的关系,观察不同核苷和核苷酸类药物治疗慢性乙型肝炎患者的血清IFNλ3水平,以及核苷和核苷酸类药物对外周血单个核细胞培养上清中IFNλ3水平的影响。满足正态性和方差齐性的计量资料多组间比较采用方差分析;对非正态或方差不齐的计量资料,两组比较采用Wilcoxon秩和检验,多组比较采用Kruskal-Wallis H检验,进一步两两比较采用Nemenyi法检验。相关性分析用Spearman相关性分析法。结果慢性乙型肝炎患者血清IFNλ3水平明显低于健康对照组[14. 7（5. 9～28. 5） pg/ml vs 76. 1（39. 4～112. 0） pg/ml,Z=-10. 114,P <0. ...
- 肝炎,乙型,慢性 /
- 干扰素类
Abstract:
Objective To investigate the serum level of interferon-λ3 ( IFN-λ3) and its clinical significance in patients with chronic hepatitis B ( CHB) , as well as its association with nucleos ( t) ide analogue ( NA) treatment. Methods A total of 119 CHB patients who were treated in The Second Affiliated Hospital of Anhui Medical University from January 2017 to May 2018 were enrolled, and 22 HBsAg-negative individuals who underwent physical examination were enrolled as healthy control group. ELISA was used to measure the serum level of IFN-λ3, which was compared between CHB patients and healthy individuals. The association between serum IFN-λ3 level and HBeAg in CHB patients was analyzed; serum IFN-λ3 level was compared between the patients treated with different NAs; the influence of NAs on the level of IFN-λ3 in the supernatant of peripheral blood mononuclear cells ( PBMCs) was analyzed. An analysis of variance was used for comparison of normally distributed continuous data with homogeneity of variance between multiple groups; the Wilcoxon rank-sum test was used for comparison of non-normally distributed continuous data or continuous data with heterogeneity of variance between two groups, the Kruskal-Wallis H test was used for comparison between multiple groups, and the Nemenyi test was used for further comparison between two groups. A Spearman correlation analysis was also performed. Results The CHB group had a significantly lower serum IFN-λ3 level than the healthy control group [14. 7 ( 5. 9-28. 5) pg/ml vs 76. 1 ( 39. 4-112. 0) pg/ml, Z =-10. 114, P < 0. 001]. In the CHB patients who did not receive antiviral therapy, serum IFN-λ3 level was negatively correlated with log HBV DNA ( r =-0. 621, P < 0. 001) . The HBeAg-negative CHB patients had a significantly higher serum IFN-λ3 level than the HBeAg-positive CHB patients [16. 4 ( 9. 0-31. 3) pg/ml vs 8. 7 ( 2. 9-23. 2) pg/ml, Z =-2. 205, P = 0. 02]. The patients who received antiviral therapy with adefovir dipivoxil had a significantly higher serum IFN-λ3 level than those who were treated with entecavir, tenofovir, telbivudine, or lamivudine ( P < 0. 05) . In vitro experiment showed that there was no significant increase in serum IFN-λ3 level in the supernatant of PBMCs treated with NAs. Conclusion CHB patients have a significantly lower serum IFN-λ3 level than healthy individuals, and the CHB patients receiving antiviral therapy with adefovir dipivoxil have a relatively high serum IFN-λ3 level.
- hepatitis B, chronic /
- interferons

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