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乙型肝炎肝硬化积证与臌胀肝肾阴虚证肠道菌群特征比较分析

蔡文君 栾雨婷 刘成海 蒋轼丽 赵长青 顾宏图 袁继丽 邢枫 周扬 吕靖 慕永平

蔡文君, 栾雨婷, 刘成海, 蒋轼丽, 赵长青, 顾宏图, 袁继丽, 邢枫, 周扬, 吕靖, 慕永平. 乙型肝炎肝硬化积证与臌胀肝肾阴虚证肠道菌群特征比较分析[J]. 临床肝胆病杂志, 2019, 35(4): 785-789. DOI: 10.3969/j.issn.1001-5256.2019.04.016.
引用本文: 蔡文君, 栾雨婷, 刘成海, 蒋轼丽, 赵长青, 顾宏图, 袁继丽, 邢枫, 周扬, 吕靖, 慕永平. 乙型肝炎肝硬化积证与臌胀肝肾阴虚证肠道菌群特征比较分析[J]. 临床肝胆病杂志, 2019, 35(4): 785-789. DOI: 10.3969/j.issn.1001-5256.2019.04.016.
Cai WenJun, Luan YuTing, Liu ChengHai, Jiang ShiLi, Zhao ZhangQing, Gu HongTu, Yuan JiLi, Xing Feng, Zhou Yang, Lu: Jing, Mu YongPing. Features of intestinal microbiota in hepatitis B cirrhosis patients with amassment or tympanites type of liver-kidney Yin deficiency syndrome: A comparative analysis[J]. J Clin Hepatol, 2019, 35(4): 785-789. DOI: 10.3969/j.issn.1001-5256.2019.04.016.
Citation: Cai WenJun, Luan YuTing, Liu ChengHai, Jiang ShiLi, Zhao ZhangQing, Gu HongTu, Yuan JiLi, Xing Feng, Zhou Yang, Lu: Jing, Mu YongPing. Features of intestinal microbiota in hepatitis B cirrhosis patients with amassment or tympanites type of liver-kidney Yin deficiency syndrome: A comparative analysis[J]. J Clin Hepatol, 2019, 35(4): 785-789. DOI: 10.3969/j.issn.1001-5256.2019.04.016.

乙型肝炎肝硬化积证与臌胀肝肾阴虚证肠道菌群特征比较分析

DOI: 10.3969/j.issn.1001-5256.2019.04.016
基金项目: 

国家自然科学基金面上项目(81573948; 81874390); 

详细信息
  • 中图分类号: R259

Features of intestinal microbiota in hepatitis B cirrhosis patients with amassment or tympanites type of liver-kidney Yin deficiency syndrome: A comparative analysis

Research funding: 

 

  • 摘要: 目的比较分析乙型肝炎肝硬化积证与臌胀肝肾阴虚证患者肠道菌群的分布差异。方法选取2016年10月-12月上海中医药大学附属曙光医院肝硬化科病房住院的乙型肝炎肝硬化积证(无腹水)肝肾阴虚证患者21例和臌胀(伴有腹水)肝肾阴虚证患者15例,收集患者粪便样本,提取DNA,采用16S rRNA测序技术测序,比较两者肠道菌群的分布差异。计数资料2组间比较采用Wilcoxon秩和检验;计量资料2组间比较采用Mann-Whitney U检验。主成分分析采用R软件包(v.2.15.3)的ADE 4软件包,根据每个样本中的操作分类单元丰度,确定是否可以区分积证肝肾阴虚证和臌胀肝肾阴虚证。组间各物种相对丰度差异的比较采用多重假设检验,采用Benjamini和Hochberg错误发现率(FDR)进行调整,Pfdr<0.05为差异有统计学意义。结果在门的水平上,与积证肝肾阴虚证组比较,臌胀肝肾阴虚证组的变形菌门的丰度显著增加(Pfdr=0.009);拟杆菌门细菌的丰度显著减少(Pfdr=0.048),放线菌门和硬壁菌门的丰度均有所减少,但2...

     

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