左旋谷氨酸单钠-大鼠-肝再生模型的创建与应用价值

李瀚旻

doi:10.3969/j.issn.1001-5256.2019.11.047

左旋谷氨酸单钠-大鼠-肝再生模型的创建与应用价值

DOI: 10.3969/j.issn.1001-5256.2019.11.047

李瀚旻

湖北省中医院肝病研究所李瀚旻名老中医专家传承工作室
湖北省中医药研究院中医基础理论研究所

基金项目:

国家自然科学基金项目(81973669,81373513,90709041,30672590,30271562,30371787)；国家科技部重大基础研究项目(“973”项目，2002CCC00300)；高等学校博士学科点专项科研基金(20124230110001)；国家十三五重大专项(2018ZX10725506)；国家中医临床研究基地(湖北)重点病种研究项目(JDZX2012054,JDZX2015172)；湖北省自然科学基金重点项目(2011CDB463)；湖北省“九五攻关”重大科技发展计划项目[鄂科技(97)30号/96IP1702]；湖北省重大研究项目(鄂教科〔2002〕6号/2002D00003)；武汉市科技局科技攻关武汉市重大科技产业化专项项目(201260523199)；李瀚旻名老中医传承工作室资助项目；李瀚旻湖北中医名师传承工作室资助项目(鄂卫生计生办通〔2018〕32号)；

详细信息

中图分类号: R575;R-332
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出版历程
- 收稿日期: 2019-05-30
- 出版日期: 2019-11-20

Establishment and application value of a monosodium glutamate-rat-liver regeneration model

Li HanMin

Institute of Hepatology,Hubei Provincial Hospital of Traditional Chinese Medicine & Li Hanmin's Inheritance Studio for Experts of Traditional Chinese Medicine
Institute of Basic Theory of Traditional Chinese Medicine,Hubei Academy of Chinese Medicine

Research funding:

摘要

摘要: 建立神经-内分泌-免疫网络功能紊乱状态下的肝再生动物模型是推进肝再生调控机制研究的迫切需要。左旋谷氨酸单钠（MSG）-大鼠-肝再生模型是将MSG-大鼠模型与部分肝脏切除法（PHx）或肝脏中毒法（CCl4等）模型结合起来创建的复合型肝再生模型,主要用于研究神经-内分泌-免疫网络功能紊乱状态下的肝再生及其调控机制,肝-脑共病相关机制及其药物筛选,亦用于病证结合相关机制及其药物疗效机制的研究。MSG-大鼠+PHx复制MSG-大鼠-肝再生模型的肝再生过程严重失调,与正常大鼠肝大部切除后的肝再生过程显著不同。采用MSG-大鼠+肝脏中毒法复制的MSG-大鼠-肝再生模型的肝纤维化严重程度较之CCl4诱导正常大鼠的肝纤维化更为显著。下丘脑-垂体-肝轴是重要的肝外大环境对肝再生的影响机制,肿瘤上皮细胞间质转分化/间质表型上皮样转化的失衡是肝再生异常（肝纤维化）重要的肝内微环境机制。通过采用MSG-大鼠-肝再生模型进行研究,初步揭示了中医药通过影响神经-内分泌-免疫-肝再生调控网络防治肝脏及其相关病证（包括肝-脑共病）的疗效及其机制,推进了病证结合的相关研究。M...
- 肝再生 /
- 模型,动物 /
- 谷氨酸钠
Abstract: The establishment of an animal model of liver regeneration in a state of neuro-endocrine-immune network dysfunction is an urgent need to promote the research on the regulatory mechanism of liver regeneration. The monosodium glutamate( MSG)-rat-liver regeneration model is a composite model of liver regeneration which combines the MSG-rat model with the model of partial hepatectomy( PHx) or hepatotoxicity( for example,CCl4),and it is mainly used to investigate liver regeneration and its regulatory mechanism in the state of neuro-endocrine-immune network dysfunction,mechanisms and drug screening of liver-brain syndrome,and mechanisms of disease-syndrome combination and drug efficacy. Dysregulated liver regeneration process is observed in the MSG-rat-liver regeneration model established by the MSG-rat model and PHx,which is significantly different from the liver regeneration process in normal rats after major hepatectomy. The MSG-rat-liver regeneration model established by the MSG-rat model and hepatotoxicity has a significantly higher degree of liver fibrosis than normal rats with CCl4-induced liver fibrosis. The hypothalamic-pituitary-hepatic axis is an important mechanism of the influence of the external environment on liver regeneration,and the imbalance of epithelial-mesenchymal transition/mesenchymal-epithelial transition is an important mechanism of the influence of intrahepatic microenvironment on abnormal liver regeneration( liver fibrosis).The research on MSG-rat-liver regeneration model preliminarily reveals the efficacy and mechanism of traditional Chinese medicine in the prevention and treatment of liver and related diseases( including liver-brain syndrome) by influencing the neuro-endocrine-immune-liver regeneration regulatory network and promotes the research on the combination of diseases and syndromes. The establishment and application of the MSG-rat-liver regeneration model has important scientific significance and application value in further exploration of the complex mechanism of liver regeneration and the mechanism of traditional Chinese medicine in regulating liver regeneration.
- liver regeneration /
- models,animal /
- sodium glutamate

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参考文献(22)

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