贵州地区HCV感染者基因型分布特征

王梅; 杨兴林; 张流; 王军; 王艺

doi:10.3969/j.issn.1001-5256.2020.02.015

贵州地区HCV感染者基因型分布特征

DOI: 10.3969/j.issn.1001-5256.2020.02.015

1. 贵阳市公共卫生救治中心肝病科
2. 贵阳市公共卫生救治中心检验科

基金项目:

贵阳市科技计划项目(筑科合同[2018]1-46号)；

详细信息

中图分类号: R512.63
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出版历程
- 收稿日期: 2019-08-09
- 出版日期: 2020-02-20

Distribution characteristics of hepatitis C virus genotypes in Guizhou,China

Department of Hepatology,Guiyang Public Health Clinical Center

Research funding:

摘要

摘要:
目的探讨贵州地区丙型肝炎患者HCV基因型分布特征,为HCV感染的防控和个体化治疗提供临床依据。方法选取2011年9月-2018年10月贵阳市公共卫生救治中心1211例HCV RNA阳性的丙型肝炎患者,采用PCR直接测序法,与GenBank中已知的HCV序列进行对比,获得HCV基因型及亚型,并分析其分布与性别、年龄、民族、地区、感染途径等因素的关系。计数资料组间比较采用χ2检验或Fisher确切概率法。结果 1211例HCV感染者共检出4种基因型和11种基因亚型,其中以1b型（26. 84%）、3b型（27. 17%）和6a型（24. 28%）为主。不同HCV基因型在男女感染者中分布差异有统计学意义（χ2=15. 428,P=0. 009）,其中男性以3b型为主（29. 34%）,女性以1b型为主（32. 21%）。不同HCV基因型分布在各年龄组中差异有统计学意义（χ2=67. 439,P <0. 001）,≤18岁组与≥60岁组以1b型为主（分别为66. 67%、58. 93%）,而19～39岁组以3b型（28. 93%）、6型（29. 29%）为主,40～59岁组以1b型（29...
- 肝炎病毒属 /
- 基因型 /
- 贵州
Abstract:
ObjectiveTo investigate the distribution characteristics of hepatitis C virus (HCV) genotypes in patients with hepatitis C in Guizhou, China, and to provide a basis for the prevention and individualized treatment of HCV infection. MethodsA total of 1211 HCV RNA-positive patients with hepatitis C who were treated in Guiyang Public Health Clinical Center from September 2011 to October 2018 were enrolled. PCR direct sequencing was performed to obtain HCV sequences, which were then compared with the known HCV sequences in GenBank to obtain HCV genotypes and subgenotypes. The association of genotype distribution with sex, age, ethnic group, region, and route of infection was analyzed. The chi-square test or the Fisher’s exact test was used for comparison of categorical data between two groups. ResultsA total of 4 genotypes and 11 subgenotypes were detected among the 1211 individuals with HCV infection, with the main genotypes of 1b (26.84%), 3b (27.17%), and 6a (24.28%). There was a significant difference in the distribution of HCV genotypes between the male and female individuals (χ2=15.428, P=0.009); 29.34% of the male individuals had genotype 3b, and 32.21% of the female individuals had genotype 1b. There was a significant difference in the distribution of HCV genotypes between different age groups (χ2=67.439, P＜0.001); genotype 1b was the main genotype in the individuals aged ≤18 years (66.67%) or ≥60 years (58.93%), genotypes 3b and 6 were the main genotypes in the individuals aged 19-39 years (28.93% and 29.29%, respectively), and genotypes 1b, 3b, and 6 were the main genotypes in the individuals aged 40-59 years (29.54%, 27.33%, and 24.28%, respectively). There was a significant difference in the distribution of HCV genotypes between the individuals with different routes of infection (χ2=153.916, P＜0.001); the most common route of infection was intravenous drug addiction (57.97%), followed by sexual contact (8.42%) and invasive cosmetic surgery (8.42%); genotype 3b was the main genotype in the individuals with intravenous drug addiction (31.48%) or invasive cosmetic surgery (32.35%), and genotype 6 was the main genotype in the individuals with sexual contact (36.27%). There was no significant difference in the distribution of HCV genotypes between the individuals in different ethnic groups or from different regions of Guizhou (both P＞0.05). ConclusionThe distribution of HCV genotypes is diverse in Guizhou, and HCV strains with genotypes 3b, 1b, and 6a are the main epidemic strains. Several rare subgenotypes of HCV genotype 6 are observed. There is a significant difference in the distribution of HCV genotypes between the individuals with different ages, sexes, or routes of infection.
- hepacivirus /
- genotype /
- Guizhou

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参考文献(23)

[1] SARRAZIN C,ZEUZEM S. Resistance to direct antiviral agents in patients with hepatitis C virus infection[J]. Gastroenterology,2010,138(2):447-462.

[2] SIMMONDS P,BUKH J,COMBET C,et al. Consensus proposals for a unified system of nomenclature of hepatitis C virus genotypes[J]. Hepatology,2005,42(4):962-973.

[3] SMITH DB,BUKH J,KUIKEN C,et al. Expanded classification of hepatitis C virus into 7 genotypes and 67 subtypes:Updated criteria and genotype assignment web resource[J].Hepatology,2014,59(1):318-327.

[4] Chinese Society of Hepatology and Chinese Society of Infectious Diseases,Chinese Medical Association. The guideline of prevention and treatment for hepatitis C:A 2015 update[J]. J Clin Hepatol,2015,31(12):1961-1979.(in Chinese)中华医学会肝病学分会，中华医学会感染病学分会．丙型肝炎防治指南(2015年更新版)[J]．临床肝胆病杂志，2015,31(12):1961-1979.

[5] Polaris Observatory HCV Collaborators. Global prevalence and genotype distribution of hepatitis C virus infection in 2015:A modelling study[J]. Lancet Gastroenterol Hepatol,2017,2(3):161-176.

[6] MESSINA JP,HUMPHREYS I,FLAXMAN A,et al. Global distribution and prevalence of hepatitis C virus genotypes[J].Hepatology,2015,61(1):77-87.

[7] BORGIA SM,HEDSKOG C,PARHY B,et al. Identification of a novel hepatitis C virus genotype from Punjab,India:Expanding classification of hepatitis C virus into 8 genotypes[J]. J Infect Dis,2018,218(11):1722-1729.

[8] MURPHY DG,SABLON E,CHAMBERLAND J,et al. Hepatitis C virus genotype 7,a new genotype originating from central Africa[J]. J Clin Microbiol,2015,53(3):967-972.

[9] SU YY,LIU HX,WANG N. Hepatitis C virus genotypes in China:A systematic review[J]. Chin J Epidemiol,2013,34(1):80-84.(in Chinese)苏迎盈，刘慧鑫，汪宁．中国丙型肝炎病毒基因型分布[J]．中华流行病学杂志，2013,34(1):80-84.

[10] HUANG CQ,WANG FX. Genotype of HCV genes in a tertiary hospital in Harbin[J/CD]. Chin J Exp Clin Infect Dis(Electronic Edition),2017,11(5):483-489.(in Chinese)黄超群，王福祥．哈尔滨某三甲医院丙型肝炎病毒基因分型研究[J/CD]．中华实验和临床感染病杂志(电子版)，2017,11(5):483-489.

[11] LIN J,WEN XY,TANG M. Hepatitis C viral genotyping and its relation with clinic among Han population in southern Sichuan area[J]. Lab Med Clin,2016,13(10):1359-1361.(in Chinese)林江，温先勇，唐敏．川南地区汉族人群丙型肝炎病毒基因分型及其与临床关系[J]．检验医学与临床，2016,13(10):1359-1361.

[12] ZHOU ZG,ZENG T,XU H,et al. Genotype distribution and clinical significance of hepatitis C virus in Chongqing[J]. J Chongqing Med Univ,2015,40(3):383-387.(in Chinese)周召刚，曾藤，徐华，等．重庆地区丙型肝炎病毒基因型分布及临床意义[J]．重庆医科大学学报，2015,40(3):383-387.

[13] DING JJ,LI YY,TIAN M. Analysis of genotypes of HCV in Guizhou by line probe nucleic acid hybeidization[J]. Chin J Exp Clin Virol,1999,13(3):243-246.(in Chinese)丁静娟，李媛媛，田苗．线探针核酸杂交分析贵州地区HCV感染株基因型[J]．中华实验和病毒学杂志，1999,13(3):243-246.

[14] YANG XL,LIANG YD,LI YF,et al. Study on hepatitis C virus genotyping in Guizhou area[J]. Chongqing Med,2014,43(30):4065-4067,4070.(in Chinese)杨兴林，梁跃东，李勇富，等．贵州地区丙型肝炎病毒基因分型的研究[J]．重庆医学，2014,43(30):4065-4067,4070.

[15] LI J,CHEN J,ZHUANG H. Epidemiology of hepatitis C[J]. J Prac Hepatol,2012,15(5):379-381.(in Chinese)李杰，陈杰，庄辉．丙型肝炎的流行病学[J]．实用肝脏病杂志，2012,15(5):379-381.

[16] FU Y,QIN W,CAO H,et al. HCV 6a prevalence in Guangdong province had the origin from Vietnam and recent dissemination to other regions of China:Phylogeographic analyses[J]. PLo S One,2012,7(1):e28006.

[17] ZHOU DX,TANG JW,CHU IM,et al. Hepatitis C virus genotype distribution among intravenous drug user and the general population in Hong Kong[J]. J Med Virol,2006,78(5):574-581.

[18] WANG Y,WU HY,ZHAO XC,et al. Evolutionary characteristics and positive selection site of hepatitis C virus isolated in intravenous drug users in Pudong new district,Shanghai[J].Chin J Epidemiol,2017,38(3):378-383.(in Chinese)王郁，吴红岩，赵希畅，等．上海市浦东新区注射吸毒者HCV的进化分析与正选择位点研究[J]．中华流行病学杂志，2017,38(3):378-383.

[19] TANG W,SU MH,JIANG JN,et al. Epidemiological characteristics and genotype distribution of hepatitis C virus in Guangxi[J]. World Chin J Dig,2014,22(9):1300-1306.(in Chinese)唐维，苏明华，江建宁，等．广西地区丙型肝炎病毒的基因型分布与流行病学特征[J]．世界华人消化杂志，2014,22(9):1300-1306.

[20] YAN Z,FAN K,WANG Y,et al. Changing pattern of clinical epidemiology on hepatitis C virus infection in southwest China[J]. Hepat Mon,2012,12(3):196-204.

[21] PENG XB,MAO XR,CHEN H. Genotypes of HCV in hepatitis C patients of Han nationality in Gansu,China[J]. J Clin Hepatol,2013,29(11):828-831.(in Chinese)彭雪彬，毛小荣，陈红．甘肃地区汉族HCV基因分型相关分析[J]．临床肝胆病杂志，2013,29(11):828-831.

[22] LI W,KANG Y,DING GQ,et al. Clinical effect and safety of sofosbuvir-ledipasvir regimen in treatment of patients with HCV genotype 6a chronic hepatitis C[J]. J Clin Hepatol,2019,35(3):526-529.(in Chinese)李威，康谊，丁岗强，等．sofosbuvir/ledipasvir方案治疗HCV6a型慢性丙型肝炎的临床效果和安全性[J]．临床肝胆病杂志，2019,35(3):526-529.

[23] YANG WW. Influencing factor on the efficacy of combined treatment of pegylated interferon-αand ribavirin in patients with chronic hepatitis C[J/CD]. Chin J Exp Clin Infect Dis(Electronic Edition),2018,12(3):301-306.(in Chinese)杨炜炜．聚乙二醇化干扰素联合利巴韦林治疗慢性丙型肝炎疗效的影响因素[J/CD]．中华实验和临床感染病杂志(电子版)，2018,12(3):301-306.

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