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Expression and significance of the long non-coding RNA EXOC7 in nonalcoholic fatty liver disease
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摘要:
目的观察非酒精性脂肪性肝病（NAFLD）患者血清及肝穿刺组织中长链非编码RNA（lncRNA） EXOC7的表达及临床意义。方法选取2013年1月1日-2018年12月31日于西南医科大学附属医院住院行肝穿刺并经影像学及组织病理学诊断为NAFLD的患者120例,其中非酒精性单纯性脂肪肝（NAFL） 47例,非酒精性脂肪性肝炎（NASH） 73例。另选取同期体检的50例无脂肪变性和脂肪性肝炎的其他肝病患者作为对照组。采用Real-time PCR法检测lncRNA EXOC7在肝组织及血清中的表达。计量资料两组间比较采用t检验;多组间比较采用方差分析,进一步两两比较采用SNK-q检验。计数资料两组间比较采用χ2检验。采用Pearson相关分析lncRNA EXOC7表达与临床各生化指标的关系。绘制受试者工作特征曲线（ROC曲线）并分析lncRNA EXOC7的临床诊断价值。结果与对照组相比,NAFL和NASH患者的组织及血清中lncRNA EXOC7表达显著增高（P值均<0. 05）,且其表达水平随着肝脏脂肪变性及炎症程度的加重而提升（F=19. 96,P <0. 05）。相...
Abstract:Objective To investigate the expression of the long non-coding RNA( lncRNA) EXOC7 in serum and liver biopsy tissue of patients with nonalcoholic fatty liver( NAFLD) and its clinical significance. Methods A total of 120 patients who underwent liver biopsy in The Affiliated Hospital of Southwest Medical University from January 1,2013 to December 31,2018 and were diagnosed with NAFLD based on imaging and histopathology were enrolled,among whom 47 had nonalcoholic fatty liver( NAFL) and 73 had nonalcoholic steatohepatitis( NASH). A total of 50 liver disease patients without steatosis or steatohepatitis were enrolled as control group. Real-time PCR was used to measure the expression of lncRNA EXOC7 in liver tissue and serum. The t-test was used for comparison of continuous data between two groups; an analysis of variance was used for comparison between multiple groups,and the SNK-q test was used for further comparison between two groups. A chi-square test was used for comparison of categorical data between groups. A Pearson correlation analysis was used to investigate the correlation between lncRNA EXOC7 and biochemical parameters. The receiver operating characteristic( ROC) curve was plotted to investigate the diagnostic value of lncRNA EXOC7. Results Compared with the control group,the patients with NAFL or NASH had significant increases in the expression of lncRNA EXOC7 in liver tissue and serum( all P < 0. 05),and the level of such expression increased with the aggravation of hepatic steatosis and inflammation( F = 19. 96,P < 0. 05). The correlation analysis showed that the expression of lncRNA EXOC7 was positively correlated with total triglyceride and low-density lipoprotein cholesterol( r = 0. 785 and r = 0. 847,both P < 0. 001) and was negatively correlated with high-density lipoprotein cholesterol and insulin sensitivity index( r =-0. 726 and-0. 709,both P < 0. 001). LncRNA EXOC7 had an area under the ROC curve of 0. 812( 95% confidence interval: 0. 599-0. 915,P < 0. 001),a sensitivity of85. 42%,and a specificity of 81. 17% in the diagnosis of NAFLD. Conclusion LncRNA EXOC7 is highly expressed in patients with NAFLD,and the expression of lncRNA EXOC7 increases with the aggravation of hepatic steatosis and inflammation,suggesting that lncRNA EXOC7 may be a potential new target for the prevention and treatment of NAFLD.
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Key words:
- non-alcoholic fatty liver disease /
- RNA,long noncoding /
- diagnosis
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