自身免疫性肝炎患儿外周血单个核细胞miRNA-181c表达与干扰素γ、趋化因子配体10、Toll样受体4的相关性分析

崔海霞; 金春梅; 吴政燮; 金爱花; 张美兰

doi:10.3969/j.issn.1001-5256.2020.10.015

自身免疫性肝炎患儿外周血单个核细胞miRNA-181c表达与干扰素γ、趋化因子配体10、Toll样受体4的相关性分析

DOI: 10.3969/j.issn.1001-5256.2020.10.015

延边大学附属医院检验科

基金项目:

2018年延边大学中青年联合基金项目(2018-35)；

详细信息

中图分类号: R725.7
计量
- 文章访问数: 1063
- HTML全文浏览量: 46
- PDF下载量: 79
- 被引次数: 0
出版历程
- 收稿日期: 2020-04-17
- 出版日期: 2020-10-20

Correlation of miRNA-181c expression in peripheral blood mononuclear cells with interferon-γ,chemokine( C-X-C motif) ligand 10,and Toll-like receptor 4 in children with autoimmune hepatitis

Department of Clinical Laboratory,The Affiliated Hospital of Yanbian University

Research funding:

摘要

摘要: 目的探讨儿童自身免疫性肝炎（AIH）患者外周血单个核细胞（PBMC） miRNA-181c表达与干扰素γ（IFNγ）、趋化因子配体10（CXCL10）、Toll样受体4（TLR4）的相关性。方法选取2015年3月-2019年5月延边大学附属医院收治的儿童AIH 27例作为AIH组,另选取同期健康体检儿童30例作为对照组,检测两组儿童PBMC miRNA-181c及IFNγ、CXCL10、TLR4表达情况。符合正态分布的计量资料两组间比较采用t检验;非正态分布的计量资料两组间比较采用Wilcoxon秩和检验。计数资料两组间比较采用χ2检验。采用Pearson相关系数分析miRNA-181c表达与各指标的相关性。采用logistic回归分析各因素对AIH的影响。结果 AIH组患儿肝功能指标AST、ALT、GGT及TBil水平均高于对照组,差异有统计学意义（t值分别为14.445、20.064、11.728、13.822,P值均<0.001）。AIH组患儿IgA、IgM及IgG水平均明显高于对照组（t值分别为7.772、5.147、6.771,P值均<0.05）。AIH组患儿PB...
- 肝炎,自身免疫性 /
- 单核细胞 /
- 微RNAs /
- 干扰素γ /
- 趋化因子CXCL10 /
- Toll样受体4
Abstract: Objective To investigate the correlation of miR-181 c expression in peripheral blood mononuclear cells( PBMCs) with interferon-γ( IFN-γ),chemokine( C-X-C motif) ligand 10( CXCL10),and Toll-like receptor 4( TLR4) in children with autoimmune hepatitis( AIH). Methods A total of 27 children with AIH who were admitted to The Affiliated Hospital of Yanbian University from March2015 to May 2019 were enrolled as AIH group,and 30 healthy children who underwent physical examination during the same period of were enrolled as control group. The expression of miR-181 c in PBMCs and the expression of IFN-γ,CXCL10,and TLR4 were measured for the two groups. The t-test was used for comparison of normally distributed continuous data between two groups,and the Wilcoxon rank-sum test was used for comparison of non-normally distributed continuous data between two groups; the chi-square test was used for comparison of categorical data between two groups. The Pearson correlation coefficient was used to investigate the correlation of miR-181 c expression with each index,and a logistic regression analysis was used to investigate the influence of each factor on AIH. Results Compared with the control group,the AIH group had significantly higher levels of the liver function parameters aspartate aminotransferase( AST),alanine aminotransferase( ALT),gamma-glutamyl transpeptidase( GGT),and total bilirubin( TBil)( t = 14. 445,20. 064,11. 728,13. 822,all P < 0. 001). The AIH group also had significantly higher levels of IgA,IgM,and IgG than the control group( t = 7. 772,5. 147,and6. 771,all P < 0. 05). The AIH group had significantly lower relative expression of miR-181 c in PBMCs than the control group( 0. 784 ±0. 173 vs 1. 106 ± 0. 224,t = 5. 819,P < 0. 05). Compared with the control group,the AIH group had significantly higher levels of IFN-γ and CXCL10 and mRNA expression of TLR4( t = 6. 949,12. 303,and 13. 835,all P < 0. 05). The correlation analysis showed that in the children with AIH,the expression of miR-181 c in PBMCs was negatively correlated with IFN-γ,CXCL10,TLR4,AST,ALT,GGT,TBil,and Ig G( r =-0. 316,-0. 348,-0. 322,-0. 427,-0. 442,-0. 408,-0. 396,and-0. 321,all P < 0. 05). The univariate logistic regression analysis showed that AST,ALT,GGT,TBil,IFN-γ,CXCL10,TLR4 mRNA,and miR-181 c were all included in the regression model( all P < 0. 05) and were the influencing factors for the onset of AIH. Conclusion Children with AIH have downregulated expression of miR-181 c in PBMCs,which is closely associated with IFN-γ,CXCL10,and TLR4,suggesting that miR-181 c may affect the development of AIH in children by regulating the immune system.
- hepatitis,autoimmune /
- monocytes /
- microRNAs /
- interferon-gamma /
- chemokine CXCL10 /
- Toll-like receptor 4

HTML全文

参考文献(20)

[1] COMERFORD M,FOGEL R,BAILEY JR,et al. Leveraging social networking sites for an autoimmune hepatitis genetic repository:Pilot study to evaluate feasibility[J]. J Med Internet Res,2018,20(1):e14.

[2] BALIETTI M,GIULI C,CONTI F. Peripheral blood brain-derived neurotrophic factor as a biomarker of alzheimer’s disease:Are there methodological biases?[J]. Mol Neurobiol,2018,55(8):6661-6672.

[3] LIMA GIACOBBO B,DOORDUIN J,KLEIN HC,et al. Brainderived neurotrophic factor in brain disorders:Focus on neuroinflammation[J]. Mol Neurobiol,2019,56(5):3295-3312.

[4] de LEMOS-BONOTTO M,VALLE-TOVO C,COSTABEBER AM,et al. A systematic review and meta-analysis of second-line immunosuppressants for autoimmune hepatitis treatment[J]. Eur J Gastroenterol Hepatol,2018,30(2):212-216.

[5] WANG T,MEN R,HU M,et al. Protective effects of Punica granatum(pomegranate)peel extract on concanavalin A-induced autoimmune hepatitis in mice[J]. Biomed Pharmacother,2018,100:213-220.

[6] YEOMAN AD,WESTBROOK RH,AL-CHALABI T,et al. Diagnostic value and utility of the simplified International Autoimmune Hepatitis Group(IAIHG)criteria in acute and chronic liver disease[J]. Hepatology,2009,50(2):538-545.

[7] ZHANG Q,WANG MH,GAN M,et al. Correlation between serum microrna-181c level and cognitive function in first-episode schizophrenia[J]. Chin J Med Offic,2019,47(9):965-966,969.(in Chinese)张强，王明洪，甘敏，等．首发精神分裂症患者血清微小RNA-181c水平与认知功能相关性分析[J]．临床军医杂志，2019,47(9):965-966,969.

[8] KANDA T,YASUI S,NAKAMURA M,et al. Interferon-free treatment for patients with chronic hepatitis C and autoimmune liver disease:Higher SVR rates with special precautions for deterioration of autoimmune hepatitis[J]. Oncotarget,2018,9(14):11631-11637.

[9] FLOREANI A,LIBERAL R,VERGANI D,et al. Autoimmune hepatitis:Contrasts and comparisons in children and adultsa comprehensive review[J]. J Autoimmun,2013,46:7-16.

[10] ABU-SHANAB A,GED Y,ULLAH N,et al. Increased incidence of post-transplant lymphoproliferative disorder in autoimmune liver disease:An Irish national experience[J]. J Clin Exp Hepatol,2018,8(1):42-49.

[11] YAO TY,CHEN WJ,DUAN XX,et al. The application of ultrasonic elastography in children’s liver disease[J]. Chin J Med Imaging,2020,28(2):146-149.(in Chinese)姚桃月，陈文娟，段星星，等．超声弹性成像在儿童肝病中的应用进展[J]．中国医学影像学杂志，2020,28(2):146-149.

[12] DONG Y,ZHU SS,ZHANG M. Pediatric autoimmune liver disease[J]. Infect Dis Info,2019,32(2):97-102.(in Chinese)董漪，朱世殊，张敏．儿童自身免疫性肝病[J]．传染病信息，2019,32(2):97-102.

[13] JIN SX,WU T,CAI FY,et al. Effect of miR-322-5p on inhibiting Th17 differentiation by targeting Akt3 in experimental autoimmune encephalomyelitis interfered by interferon-β[J].J Shanghai Jiaotong Univ(Med Sci),2019,39(8):834-842.(in Chinese)金书欣，吴婷，蔡飞扬，等．miR-322-5p靶向Akt3抑制Th17分化对干扰素β干预实验性自身免疫性脑脊髓炎的影响[J]．上海交通大学学报(医学版)，2019,39(8):834-842.

[14] LUO YP,YANG ZC. Role of microRNA in autoimmune and vasculitis[J]. Chin J Immunol,2019,35(2):250-256.(in Chinese)罗叶萍，杨作成．microRNA在自身免疫及血管炎性疾病发病中的作用[J]．中国免疫学杂志，2019,35(2):250-256.

[15] ZHANG Z,XUE Z,LIU Y,et al. MicroRNA-181c promotes Th17 cell differentiation and mediates experimental autoimmune encephalomyelitis[J]. Brain Behav Immun,2018,70:305-314.

[16] LEI YJ,YANG JH. The association between interleukin-8 and autoimmune liver diseases[J]. J Clin Hepatol,2019,35(2):411-413.(in Chinese)雷云洁，杨晋辉．IL-8与自身免疫性肝病的关系[J]．临床肝胆病杂志，2019,35(2):411-413.

[17] SUN MY,LIU WT. Role of regulatory T cells in pathogenesis and therapy of autoimmune liver disease[J]. World Chin J Dig,2019,27(11):665-670.(in Chinese)孙孟宇，刘文天．调节性T细胞在自身免疫性肝病中的作用机理及治疗进展[J]．世界华人消化杂志，2019,27(11):665-670.

[18] COAD M,DOYLE M,STEINBACH S,et al. Simultaneous measurement of antigen-induced CXCL10 and IFN-γenhances test sensitivity for bovine TB detection in cattle[J]. Vet Microbiol,2019,230:1-6.

[19] ZHANG YM,YAN RM,DING ML,et al. TLR2 blockade on dendritic cell inhibits autoimmune diabetes[J]. Chin J Immunol,2019,35(8):902-905,911.(in Chinese)张延梅，严瑞明，丁玫琳，等．树突状细胞TLR2阻断抑制自身免疫性糖尿病研究[J]．中国免疫学杂志，2019,35(8):902-905,911.

[20] LEE JH,KIM B,JIN WJ,et al. Pathogenic roles of CXCL10signaling through CXCR3 and TLR4 in macrophages and T cells:Relevance for arthritis[J]. Arthritis Res Ther,2017,19(1):163.

施引文献

资源附件(0)

访问统计