山萘酚对人肝癌Bel-7402细胞增殖、迁移、侵袭及凋亡的影响

仲富瑞; 程宦立; 张浩; 杜毅超; 胡启辉; 付文广; 夏先明

doi:10.3969/j.issn.1001-5256.2020.12.017

山萘酚对人肝癌Bel-7402细胞增殖、迁移、侵袭及凋亡的影响

DOI: 10.3969/j.issn.1001-5256.2020.12.017

1. 西南医科大学附属医院肝胆外科
2. 西南医科大学附属医院四川省院士(专家)工作站

基金项目:

2017年第四批省级科技计划-重点研发计划项目（2017SZYZF0015）；

详细信息

中图分类号: R285.5
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出版历程
- 收稿日期: 2020-06-11
- 出版日期: 2020-12-20

Effect of kaempferol on the proliferation,migration,invasion,and apoptosis of human hepatoma Bel-7402 cells

Department of Hepatobiliary Surgery,The Affiliated Hospital of Southwest Medical University
Academician (Expert) Workstation of Sichuan Province,The Affiliated Hospital of Southwest Medical University

Research funding:

摘要

摘要:
目的探讨山萘酚对人肝癌Bel-7402细胞增殖、迁移、侵袭及凋亡的影响,并探讨其相关分子机制。方法将体外培养的肝癌Bel-7402细胞随机分为对照组和低、中、高浓度实验组,实验组分别用低、中、高浓度山萘酚（25、50、100μmol/L）处理,对照组予以等量二甲基亚砜处理。用CCK-8法检测山萘酚对Bel-7402细胞活力的影响;平板克隆检测山萘酚对细胞克隆形成能力的影响;细胞划痕和Transwell小室侵袭实验检测山萘酚对细胞迁移和侵袭的影响; Western Blot检测凋亡及周期相关蛋白表达情况。多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验。结果对照组和低、中、高浓度实验组的细胞活力在处理24 h后分别为（100. 00±2. 72）%、（75. 70±2. 42）%、（62. 79±2. 45）%、（43. 41±2. 11）%,与对照组比较,各实验组细胞活力均明显下降（P值均<0. 05）。各组细胞克隆形成数分别为923. 3±35. 2、682. 7±24. 4、464. 0±22. 0、327. 3±14. 0,与对照组比较,各实验组细胞克隆形成能...
- 山萘酚 /
- 肝肿瘤 /
- 细胞增殖 /
- 细胞运动 /
- 细胞凋亡
Abstract:
Objective To investigate the effect of kaempferol on the proliferation,migration,invasion,and apoptosis of human hepatoma Bel-7402 cells and related molecular mechanism. Methods Hepatoma Bel-7402 cells cultured in vitro were randomly divided into control group and low-,middle-,and high-concentration experimental groups. The experimental groups were treated with low-,middle-,and high-concentration kaempferol( 25,50,and 100 μmol/L),and the control group was treated with an equal volume of dimethyl sulfoxide. CCK-8 assay was used to observe the effect of kaempferol on the viability of Bel-7402 cells; plate colony formation assay was used to evaluate the effect of kaempferol on cell colony formation ability; wound healing assay and Transwell chamber were used to observe the effect of kaempferol on cell migration and invasion; Western blot was used to measure the expression of apoptosis-and cycle-related proteins. A one-way analysis of variance was used for comparison between multiple groups,and the least significant difference t-test was used for further comparison between two groups. Results After 24 hours of treatment,the cell viability was 100. 00% ± 2. 72% in the control group and 75. 70% ± 2. 42%,62. 79% ± 2. 45%,and 43. 41% ± 2. 11%,respectively,in the low-,middle-,and high-concentration experimental groups,and compared with the control group,the experimental groups had a significant reduction in cell viability( all P < 0. 05).The number of cell colonies was 923. 3 ± 35. 2 in the control group and 682. 7 ± 24. 4,464. 0 ± 22. 0,and 327. 3 ± 14. 0,respectively,in the low-,middle-,and high-concentration experimental groups,and compared with the control group,the experimental groups had a significant reduction in cell colony formation ability( all P < 0. 05). After 24 hours of treatment,the relative migration rate was 100. 00% ± 1. 11% in the control group and 63. 33% ± 1. 16%,51. 72% ± 3. 23%,and 37. 18% ± 2. 71%,respectively,in the low-,middle-,and high-concentration experimental groups,and the number of transmembrane cells was 212. 0 ± 3. 0 in the control group and 134. 0 ± 2. 0,71. 0 ±2. 0,and 34. 0 ± 1. 0,respectively,in the low-,middle-,and high-concentration experimental groups; compared with the control group,the experimental groups had significant reductions in relative migration rate and number of transmembrane cells( all P < 0. 05). After48 hours of treatment,compared with the control group,the low-,middle-,and high-concentration experimental groups had a significant reduction in the expression of the anti-apoptotic protein Bcl-2( all P < 0. 05),a significant increase in the expression of the pro-apoptotic protein Bax( all P < 0. 05),and a significant reduction in the expression of C < italic/> yclinD1( all P < 0. 05). Conclusion Kaempferol can inhibit the proliferation,migration,and invasion of human hepatoma Bel-7402 cells and promote the apoptosis of such cells,possibly by regulating the apoptosis proteins Bax and Bcl-2 and downregulating the expression of CyclinD1.
- kaempferol /
- liver neoplasms /
- cell proliferation /
- cell movement /
- apoptosis

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