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肝细胞癌患者肝动脉化疗栓塞术后外周血长链非编码RNA TINCR水平与Th1/Th2平衡及预后的相关性分析

柏涛 王静丽 胡旭东 夏冰 程海林 王娟

引用本文:
Citation:

肝细胞癌患者肝动脉化疗栓塞术后外周血长链非编码RNA TINCR水平与Th1/Th2平衡及预后的相关性分析

DOI: 10.3969/j.issn.1001-5256.2021.01.016
基金项目: 

武汉市卫生与计划生育委员会科研课题 (WX12D24)

利益冲突声明:本研究不存在研究者、伦理委员会成员、受试者监护人以及与公开研究成果有关的利益冲突,特此声明。
作者贡献声明:柏涛、王静丽负责课题设计,资料分析,撰写论文;夏冰、程海林、王娟参与收集数据,修改论文;胡旭东负责拟定写作思路,指导撰写文章并最后定稿。
详细信息
    作者简介:

    柏涛(1981—),男,主治医师,主要从事肝病的临床治疗及基础研究

    通信作者:

    柏涛,baitao811027@163.com

  • 中图分类号: R735.7

Association of the expression level of the long non-coding RNA TINCR in peripheral blood with Th1/Th2 balance and prognosis after transcatheter arterial chemoembolization in patients with hepatocellular carcinoma

  • 摘要:   目的  探讨分析外周血长链非编码RNA(lncRNA) TINCR表达水平与肝细胞癌(HCC)患者行肝动脉化疗栓塞术(TACE)术后辅助性T淋巴细胞1(Th1)/Th2平衡及预后的相关性。  方法  回顾性选取2015年3月—2017年3月在湖北省武汉市金银潭医院行TACE治疗的HCC患者85例,并随机抽取在本院体检的健康志愿者30例作为对照组。检测HCC患者TACE完成后7 d及对照组外周血lncRNA TINCR表达情况、Th1/Th2细胞因子表达情况。计量资料组间比较采用t检验,计数资料组间比较采用χ2检验,等级资料组间比较采用Kruskal-Wallis H检验。lncRNA TINCR相对表达量与Th1/Th2细胞因子的相关性采用Spearman相关性分析。lncRNA TINCR相对表达量对生存情况的影响采用Kaplan-Meier生存曲线分析,生存曲线比较采用log-rank检验。  结果  HCC组患者外周血lncRN TINCR相对表达量(1.784±0.429)高于对照组(0.926±0.263),差异有统计学意义(t=10.277,P<0.05)。lncRNA TINCR相对表达与HCC患者血管侵犯、肿瘤大小、肿瘤分期及血清AFP水平有关(t值分别为3.958、4.499、3.361、4.949,P值分别为<0.001、<0.001、0.001、<0.001)。HCC组患者血清IFNγ、IL-2水平明显低于对照组[(13.48±5.20) pg/ml vs (27.49±7.21) pg/ml、(21.89±6.45) pg/ml vs (32.05±7.89) pg/ml,t值分别为11.408、6.986,P值均<0.05],而IL-4、IL-10水平明显高于对照组[(13.73±3.35) pg/ml vs (9.36±2.73) pg/ml、(12.75±2.74) pg/ml vs (8.93±2.16) pg/ml,t值分别为6.426、6.909,P值均<0.05]。HCC患者外周血lncRNA TINCR相对表达量与血清IFNγ、IL-2水平呈负相关(r值分别为-3.164、-3.270,P值均<0.001),与患者血清IL-4、IL-10呈正相关(r值分别为2.963、3.044,P值分别为0.007、<0.001)。以lncRNA TINCR相对表达量≥1.700作为高表达,85例HCC患者中lncRNA TINCR高表达患者47例,低表达患者38例。log-rank检验结果显示,lncRNA TINCR低表达患者生存情况明显优于高表达患者(χ2=4.182, P<0.05)。  结论  HCC患者行TACE后外周血lncRNA TINCR表达明显高于健康人群,lncRNA TINCR表达与患者病理特征及Th1/Th2免疫平衡有密切联系,可作为患者生存预后的潜在预测指标之一。

     

  • 图  1  lncRNA TINCR相对表达量与患者预后关系

    表  1  lncRNA TINCR相对表达量与HCC患者病理特征分析

    项目 例数 lncRNA TINCR相对表达量 统计值 P
    性别 t=1.148 0.254
      男 46 1.895±0.318
      女 39 1.972±0.296
    年龄 t=0.798 0.427
      ≥50岁 49 1.964±0.304
      <50岁 36 1.912±0.287
    Child-Pugh分级 Z=0.864 0.433
      A级 26 1.974±0.412
      B级 53 2.061±0.397
      C级 6 1.875±0.332
    血管侵犯 t=3.958 <0.001
      有 64 2.108±0.227
      无 21 1.864±0.295
    肿瘤大小 t=4.499 <0.001
      >5 cm 51 2.175±0.304
      ≤5 cm 34 1.903±0.218
    临床分期 t=3.361 0.001
      Ⅱ期 44 1.764±0.275
      Ⅲ期 41 1.974±0.301
    肝炎病史 t=0.293 0.770
      有 67 1.922±0.341
      无 18 1.896±0.307
    血清AFP t=4.949 <0.001
      <400 ng/ml 33 1.816±0.197
      ≥400 ng/ml 52 2.095±0.283
    下载: 导出CSV

    表  2  两组受试者Th1/Th2细胞因子水平比较

    组别 例数 IFNγ(pg/ml) IL-2(pg/ml) IL-4(pg/ml) IL-10(pg/ml)
    HCC组 85 13.48±5.20 21.89±6.45 13.73±3.35 12.75±2.74
    对照组 30 27.49±7.21 32.05±7.89 9.36±2.73 8.93±2.16
    t 11.408 6.986 6.426 6.909
    P <0.05 <0.05 <0.05 <0.05
    下载: 导出CSV
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  • 收稿日期:  2020-04-30
  • 录用日期:  2020-08-21
  • 出版日期:  2021-01-20
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