单核细胞/高密度脂蛋白胆固醇与非酒精性脂肪性肝病的相关性分析

王娟; 王迎春; 王春宇

doi:10.3969/j.issn.1001-5256.2021.05.031

单核细胞/高密度脂蛋白胆固醇与非酒精性脂肪性肝病的相关性分析

DOI: 10.3969/j.issn.1001-5256.2021.05.031

大连大学附属中山医院消化二科，辽宁大连 116001

基金项目:

大连大学博士启动专项基金 (20181QL005)

利益冲突声明：本研究不存在研究者、伦理委员会成员、受试者监护人以及与公开研究成果有关的利益冲突。

作者贡献声明：王娟负责课题设计，资料分析，论文撰写；王春宇参与数据收集；王迎春修改论文及最后定稿。

详细信息

作者简介:
王娟(1993—)，女，主要从事非酒精性脂肪肝及胃肠肝胆病相关的研究

通信作者:
王迎春，wych_1648@126.com

中图分类号: R575.5
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出版历程
- 收稿日期: 2020-07-30
- 录用日期: 2020-08-31
- 出版日期: 2021-05-20

Association between monocyte-to-high-density lipoprotein cholesterol ratio and nonalcoholic fatty liver disease

Second Department of Gastroenterology, Zhongshan Hospital Affiliated to Dalian University, Dalian, Liaoning 116001, China

摘要

摘要: 目的探讨单核细胞/高密度脂蛋白胆固醇(MHR)与非酒精性脂肪性肝病(NAFLD)的关系。方法选取2018年1月—2020年10月入住大连大学附属中山医院消化内科并经腹部CT诊断为NAFLD的208例患者作为NAFLD组，另选取同期210例健康体检人群设为对照组。所有受试者均行血常规、生化及腹部CT检查，计算血清MHR水平。另外，根据腹部CT的影像学结果，将NAFLD患者分为轻度NAFLD组(n=148)及中重度NAFLD组(n=60)，观察不同程度NAFLD患者与对照组WBC及MHR等指标的差异。正态分布计量资料两组间比较采用独立样本t检验，多组间比较采用单因素方差分析。偏态分布计量资料两组间比较采用Mann-Whitney U秩和检验，多组间采用Kruskal-Wallis H检验。计数资料两组间比较采用四格表χ²检验，3组间比较采用R×C表χ²检验。MHR与各代谢指标及NAFLD严重程度间的相关性采用Spearman相关分析。绘制受试者工作特征曲线评估MHR对NAFLD的诊断价值。结果与对照组相比，NAFLD组中的体质量(t=-10.573, P < 0.001)、BMI(t=-13.112, P < 0.001)、吸烟史(χ²=14.667, P < 0.001)、WBC(t=-7.359, P < 0.001)、单核细胞(Z=-9.932, P < 0.001)、LDL-C(t=-3.394, P=0.001)、TG(Z=-11.737, P < 0.001)、CHO(t=-2.985, P=0.003)、空腹血糖(Z=-7.827, P < 0.001)、ALT(Z=-12.583, P < 0.001)、AST(Z=-9.514, P < 0.001) 水平均增加，而血清HDL-C(t=10.440, P < 0.001)水平下降；另外，MHR水平存在性别差异，男性明显高于女性，差异有统计学意义(P < 0.001)。与对照组及轻度NAFLD组相比，血清MHR水平在中重度NAFLD组中显著升高，差异有统计学意义(P值均 < 0.001)。相关分析结果提示血清MHR水平与HDL-C呈负相关(r=-0.565, P < 0.001)，与吸烟史、体质量、BMI、WBC、单核细胞、TG、空腹血糖、ALT、AST呈正相关(r值分别为0.449、0.482、0.430、0.478、0.892、0.333、0.157、0.386、0.281，P值均 < 0.01)。同时，MHR水平与NAFLD严重程度呈正相关(r=0.629，P < 0.001)。ROC曲线表明MHR曲线下面积为0.846(95%CI：0.810~0.882，P < 0.001)，敏感度和特异度分别为77.9%和74.3%。结论血清MHR水平与NAFLD相关，可作为评价NAFLD病情进展的一种预测指标。
- 非酒精性脂肪性肝病 /
- 单核细胞 /
- 胆固醇, HDL
Abstract: Objective To investigate the association between monocyte-to-high-density lipoprotein cholesterol ratio (MHR) and nonalcoholic fatty liver disease (NAFLD). Methods A total of 208 patients who were admitted to Department of Gastroenterology, Zhongshan Hospital Affiliated to Dalian University, from January 2018 to October 2020 and were diagnosed with NAFLD by abdominal CT were enrolled as NAFLD group, and 210 healthy individuals were enrolled as control group. All subjects underwent routine blood test, biochemical examination, and abdominal CT examination, and serum MHR was calculated. In addition, according to abdominal CT findings, the patients with NAFLD were divided into mild NAFLD group with 148 patients and moderate-to-severe NAFLD group with 60 patients, and the variables such as white blood cell count (WBC) and MHR were compared between the three groups. The independent samples t-test was used for comparison of normally distributed continuous data between two groups, and a one-way analysis of variance was used for comparison between multiple groups; the Mann-Whitney U test was used for comparison of data with skewed distribution between two groups, and the Kruskal-Wallis H test was used for comparison between multiple groups. The fourfold table chi-square test was used for comparison of categorical data between two groups, and the R×C table chi-square test was used for comparison between three groups. A Spearman correlation analysis was used to investigate the correlation of MHR with metabolic markers and the severity of NAFLD. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of MHR in predicting NAFLD. Results Compared with the control group, the NAFLD group had significant increases in body weight (t=-10.573, P < 0.001), body mass index (BMI) (t=-13.112, P < 0.001), smoking history (Z=14.667, P < 0.001), WBC (t=-7.359, P < 0.001), monocytes (Z=-9.932, P < 0.001), low-density lipoprotein cholesterol (t=-3.394, P=0.001), triglyceride (TG) (Z=-11.737, P < 0.001), cholesterol (t=-2.985, P=0.003), fasting blood glucose (FBG) (Z=-7.827, P < 0.001), alanine aminotransferase (ALT) (Z=-12.583, P < 0.001), and aspartate aminotransferase (AST) (Z=-9.514, P < 0.001) and a significant reduction in serum high-density lipoprotein cholesterol (HDL-C) (t=10.440, P < 0.001); in addition, MHR level had gender differences, and male patients had a significantly higher level than female patients (P < 0.001). Compared with the control group and the mild NAFLD group, the moderate-to-severe NAFLD group had a significant increase in serum MHR level (P < 0.001). The correlation analysis showed that serum MHR level was negatively correlated with HDL-C (r=-0.565, P < 0.001) and were positively correlated with smoking history, body weight, BMI, WBC, monocytes, TG, FBG, ALT, and AST (r=0.449, 0.482, 0.430, 0.478, 0.892, 0.333, 0.157, 0.386, and 0.281, all P < 0.01). At the same time, MHR level was positively correlated with the severity of NAFLD (r=0.629, P < 0.001). The ROC curve showed that MHR had an area under the ROC curve of 0.846 (95% confidence interval: 0.810-0.882, P < 0.001), with a sensitivity of 77.9% and a specificity of 74.3%. Conclusion Serum MHR level is associated with NAFLD and can be used as a predictive index for evaluating the progression of NAFLD.
- Non-alcoholic Fatty Liver Disease /
- Monocytes /
- Cholesterol, HDL

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图 1 对照组与NAFLD组患者血清MHR的比较

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图 2 对照组与不同程度NAFLD组MHR水平的比较

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图 3 MHR水平在不同性别间比较

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图 4 MHR水平在不同亚组性别内比较

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图 5 MHR诊断NAFLD的ROC曲线

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表 1 对照组与NAFLD组患者一般情况的比较

指标	对照组(n=210)	NAFLD组(n=208)	统计值	P值
男/女(例)	95/115	114/94	χ²=3.828	>0.050
年龄(岁)	52(44~61)	55(43~62)	Z=-1.066	0.286
吸烟[例(%)]	30(14.29)	62(29.81)	χ²=14.667	< 0.001
身高(cm)	167(162~172)	170(162~175)	Z=-1.891	0.059
体质量(kg)	64.64±10.83	77.80±14.35	t=-10.573	< 0.001
BMI(kg/m²)	22.88±2.87	27.03±3.56	t=-13.112	< 0.001
WBC(×10⁹/L)	5.11±1.14	6.00±1.31	t=-7.359	< 0.001
单核细胞(×10⁹/L)	0.16(0.13~0.20)	0.23(0.20~0.30)	Z=-9.932	< 0.001
HDL-C(mmol/L)	1.45±0.30	1.18±0.22	t=10.440	< 0.001
MHR	0.12(0.08~0.16)	0.20(0.16~0.25)	Z=-12.233	< 0.001
LDL-C(mmol/L)	2.88±0.68	3.13±0.78	t=-3.394	0.001
TG(mmol/L)	1.15(0.87~1.44)	1.92(1.49~2.86)	Z=-11.737	< 0.001
CHO(mmol/L)	4.99±0.90	5.28±1.02	t=-2.985	0.003
FBG(mmol/L)	4.88(4.55~5.21)	5.34(4.89~6.06)	Z=-7.827	< 0.001
ALT(U/L)	16.0(12.0~24.0)	36.0(25.0~54.5)	Z=-12.583	< 0.001
AST(U/L)	18.00(15.00~21.00)	24.00(18.00~30.75)	Z=-9.514	< 0.001

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表 2 对照组与不同程度NAFLD患者一般情况的比较

指标	对照组(n=210)	轻度NAFLD组(n=148)	中重度NAFLD组(n=60)	统计值	P值
男/女(例)	95/115	75/73	39/21	χ²=7.332	0.026
年龄(岁)	51.40±11.78	53.61±11.50	50.43±13.32	F=2.141	0.119
吸烟[例(%)]	30(14.29)	36(24.32)	26(43.33)	H=23.654	< 0.001
身高(cm)	167.0(162.0~172.0)	169.0(161.3~175.0)	170.5(162.0~177.0)	H=5.292	0.071
体质量(kg)	64.64±10.83	76.67±12.84	80.60±17.36	F=58.503	< 0.001
BMI(kg/m²)	22.88±2.87	26.81±3.18	27.58±4.35	F=129.920	< 0.001
WBC(×10⁹/L)	5.11±1.14	5.79±1.17	6.51±1.49	F=54.130	< 0.001
单核细胞(×10⁹/L)	0.16(0.13~0.20)	0.21(0.19~0.26)	0.30(0.22~0.35)	H=115.697	< 0.001
HDL-C(mmol/L)	1.45±0.30	1.21±0.23	1.13±0.19	F=88.936	< 0.001
MHR	0.12(0.08~0.16)	0.19(0.15~0.22)	0.26(0.20~0.32)	H=167.607	< 0.001
LDL-C(mmol/L)	2.88±0.68	3.18±0.79	2.99±0.75	F=7.180	0.001
TG(mmol/L)	1.15(0.87~1.44)	1.91(1.53~2.82)	1.96(1.45~3.11)	H=137.865	< 0.001
CHO(mmol/L)	4.99±0.90	5.33±0.99	5.14±1.11	F=5.278	0.005
FBG(mmol/L)	4.88(4.55~5.21)	5.34(4.87~6.05)	5.35(4.94~6.06)	H=61.521	< 0.001
ALT(U/L)	16.00(12.00~24.00)	32.00(21.78~50.00)	46.00(32.35~60.75)	H=167.151	< 0.001
AST(U/L)	18.00(15.00~21.00)	23.00(17.25~29.00)	24.50(21.00~33.75)	H=95.963	< 0.001

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表 3 血清MHR水平与各变量的相关性

变量	r值	P值
年龄	-0.094	0.054
吸烟史	0.449	< 0.001
体质量	0.482	< 0.001
BMI	0.430	< 0.001
WBC	0.478	< 0.001
单核细胞	0.892	< 0.001
HDL-C	-0.565	< 0.001
LDL-C	0.013	0.781
TG	0.333	< 0.001
CHO	-0.071	0.145
FBG	0.157	0.001
ALT	0.386	< 0.001
AST	0.281	< 0.001
NAFLD严重性	0.629	< 0.001

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