乙肝患者外周血单个核细胞中乙肝病毒复制中间产物RNA的检测及临床意义

张凯宇; 王峰; 娄金风; 蔡艳俊; 吕美德

乙肝患者外周血单个核细胞中乙肝病毒复制中间产物RNA的检测及临床意义

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中图分类号: R512.62
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出版历程
- 出版日期: 2000-02-20

Detection of Hepatitis B virus Replicative Intermediates RNA in Peripheral Blood Mononuclear cells in patients with Hepatitis B virus Infection and its clinical significance

摘要

摘要: 采用逆转录 -多聚酶链反应技术检测乙肝患者外周血单个核细胞乙肝病毒复制中间产物RNA。 6 0例乙肝患者外周血单个核细胞中乙肝病毒复制中间产物RNA阳性率为 8 3% （5 / 6 0 ） ,其中 30例慢性肝炎患者阳性率为 13 3% （4/ 30 ） ,15例肝炎肝硬变患者阳性率为 6 7% （1/ 15 ） ,15例急性肝炎患者中无 1例阳性。认为外周血单个核细胞中乙肝病毒复制中间产物与乙肝的慢性化有关 ,但与临床病情轻重无关
- 外周血单个核细胞 /
- 乙型肝炎病毒 /
- 乙型肝炎病毒复制中间产物RNA /
- 逆转录-多聚酶链反应
Abstract: WT5”BZ]We detected hepatitis B virus replicative intermediates RNA in peripheral blood mononuclear cells in patients with hepatitis B virus infection by RT-PCR technology A positive rate of 8 3% was detected for hepatitis B virus replicative intermediates RNA in peripheral blood mononuclear cells in 60 cases of patients with hepatitis B virus infection The positive rate was 13 3% in 30 cases of chronic hepatitis, and it was 6 7% in 15 cases of cirrhosis No one case was detected in 15 cases of acute hepatits This indicated that the replication of hepatitis B virus in peripheral blood mononuclear cells was related to the chronicity of hepatitis B, but was not related to the seriousity of ill [WT5”HZ]
- WT5”BZ]Peripheral Blood Mononuclear cells Hepatitis B virus Hepatitis B virus Replicative Intermediate RNA Reverse Transcription-Polymerase Chain Reaction /

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参考文献(7)

[]〔1〕DavisonF , AlexanderGJM , AnastassakosC , etal LeucocytehepatitisBvirusDNAinacuteandchronichepatitisBvirusinfection JournalofMedicalVirology , 1987, 2 2∶3 79

[]〔2〕ChomczynskiPandSacchiN Single -stepmethodofRNAisolationbyacidguaridiniumthiocyanate -phenol-chloroformextraction AnalBiochem , 1987, 162∶15 6

〔3〕分子克隆实验指南　第 2版。 1992 , 681

〔4〕PasqunelliC HBVDNAinperipheralmononuclearbloodcells:Relation shipwithviralreplication Abstracton 1988meetingonmolecularbiologyofHBV , ColdspringHarbor , 1988, P118

[]〔5〕刘克洲 , 陈慰浙 , 何文南 , 等。血清和肝组织内HBV复制状态与肝脏病变活动性关系的研究。浙江医科大学学报 , 1993 , 2 2 ( 6) ∶2 64

〔6〕MoraledaG , BartolomeJ, MartinezMG , etal InfluenceofhepatitisdeltavirusreplicationinthepresenceofhepatitisBvirusDNAinperipheralbloodmononuclercells Hepatology, 1990 , 12∶12 90〔7〕Ferayc , ZignegoAl, SamuelD , etal PersistenthepatitsBvirusinfectionofmononuclearbloodcellswithoutconcomitantliverinfection Transplantation , 1990 , 49∶115 5

[]〔8〕HarrisonT HepatitisBvirusRNAinperipheralbloodleakocytes:abriefreview JMedVirol, 1990 , 3 1∶3 3

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