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The primary study of expression and function of CK2α subunit in liver cancer
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摘要: 为了克隆肝癌中CK2α基因并研究其功能。通过逆转录PCR从肝癌组织及癌旁肝组织中获得CK2α亚基因编码区cDNA。利用表达载体pT7- 7进行定向克隆。IPTG诱导表达 ,Westernblot鉴定。CK2α在肝癌中表达强于癌旁肝组织。转化子的阳性筛选率为 10 0 %。重组质粒转化表达菌经IPTG诱导后出现一个分子量 4 2kDa蛋白过度表达 ,Westernblot结果证明 ,过度表达产物能与抗人CK2α亚基抗体发生特异性免疫反应。结论 蛋白激酶CK2α亚基在肝癌中表达 ,并产生一定的功能Abstract: To study the expression and function of CK2α subunit in liver cancer.The aim cDNA was obtained from liver cancer and its paracancerous tissue by RT-PCR.It was directly cloned into pT7-7 expression vector, expressed by inducing with IPTG.The products were identified with Western blotting.The expression subunit in liver cancer was higher than that in its paracancerous tissue.The positive rate of transformants was 100%.One protein of 42kDa was overexpressed by inducing with IPTG.The western blot confirmed that the recombinant product could specially react with antibody against human CK2α subunit.CK2α subunit was expressed in liver cancer and resulted in function.
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Key words:
- CK2α subunit /
- Hepatocellular carcinoma /
- Molecular cloning /
- Western blot /
- Gene experssion
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[1]Pinna LA, Meggio F.Protein kinase CK2 (casein Kinase-2) and its implocation in cell division and proliferation[J].Prog Cell Cycle Res, 1997, 3∶77-97. [2]Tavfic S.Yu S, Wang H, et al.Protein Kinase CK2 signal in neoplasia[J].Histol Histopathol, 2001, 16 (2) ∶573-582. -
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