人胎肝干细胞体外分离、培养及鉴定

高沿航; 李玉林; 李艳茹; 吴珊; 全成实; 王芯蕊; 张丽红

人胎肝干细胞体外分离、培养及鉴定

1. 吉林大学第一医院消化内科
2. 吉林大学基础医学院病理解剖学教研室

基金项目:

“863”计划重大专项(2004AA205020)；

详细信息

中图分类号: R329
计量
- 文章访问数: 2391
- HTML全文浏览量: 22
- PDF下载量: 839
- 被引次数: 0
出版历程
- 出版日期: 2007-01-20

Isolation, culture, identification of human fetal liver stem cells in vitro

Research funding:

摘要

摘要: 研究人胎肝干细胞体外分离纯化方法,并对其进行初步鉴定及生物学特性分析。采用胶原酶消化、重力沉降及密度梯度离心方法分离人胎肝干细胞,通过免疫细胞化学方法对其进行初步鉴定,以及应用流式细胞仪等对其生长状况进行评估。所获得的原代细胞共传8代,细胞呈小梭形或三角形,体积较小,胞核较大,而胞浆较少;第4代细胞中进入生长期的细胞约占88.2%;免疫细胞化学染色显示细胞胞质中ALB、CK19染色阳性。人胎肝中存在具有肝干细胞特征的细胞,它们可能会成为细胞移植及肝移植良好的细胞资源。
- 人胎 /
- 肝干细胞 /
- 免疫细胞化学 /
- 生物学特性
Abstract: To explore the methods of isolation and purification of human fetal liver stem cells in vitro and analyze the bionomics.The fetal liver stem cells were isolated by collagenase digestion, gravity sedimentation and density gradient centrifugation, identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition.The primary cell propagated 8 times and were small fusiform or triangle and smaller than hepatocytes with larger nucleus and less plasma;it was 88.2% in the growing period of 4th generation cells and the stains of ALB and CK19 were positive in Immunocytochemical.The liver stem cells exist in the human fetal liver and maybe become the good cell resources for cell transplantation and liver transplantation.
- human fetus /
- liver stem cell /
- immunocytochemistry /
- bionomics

HTML全文

参考文献(11)

[1]Bilir BM, Guinette D, Karrer F, et al.Hepatocyte transplantation inacute liver failure[J].Liver Transpl, 2000, 6∶32-40.

[2]Strom SC, Chowdhury JR, Fox IJ.Hepatocyte transplantation for thetreatment of human disease[J].Semin Liver Dis, 1999, 19∶39-48.

[3]Kobayashi N, Ito M, Nakamura J, et al.Hepatocyte transplantationimproves liver function and prolongs survival in rats with decompen-sated liver cirrhosis[J].Transplant Proc, 1999, 31∶428-429.

[4]Kobayashi N, Fujiwara T, Westerman KA, et al.Prevention of acuteliver failure in rats with reversibly immortalized human hepatocytes[J].Science, 2000, 287∶1258-1262.

[5]Weglarz TC, Degen JL, Sandgren EP.Hepatocyte transplantation in-to diseased mouse liver.Kinetics of parenchymal repopulation and i-dentification of the proliferation capacity of tetraploid and octaploidhepatocytes[J].Am J Pathol, 2000, 157∶1963-1974.

[6]Selglen PO.Preparation of isolated rat liver cells[J].Methods CellBiol, 1976, 13∶29-83.

[7]Suzudi A, Zheng Y, Kondo R, et al.Flow-cytometric separationand enrichment of hepatic progenitor cells in developing mouse liver[J].HEPATOLOGY, 2000, 32∶1230-1239.

[8]Kubota H, Reid LM.Clonogenic hepatoblasts, common precursorsfor hepatocytic and biliary lineages, are lacking classical major histo-compatibility complex classⅠantigen[J].Proc Natl Acad Sci USA, 2000, 97∶12132-12137.

[9]Hirose T, Yasuchika K, Fujikawa T, et al.“Blastophere”:a newculture method for human fetal hepatic progenitor cells[J].Gastroen-terology, 2002, 120 (Suppl1) ∶A-542.

[10]Kamiya A, Kojima N, Kinoshita T, et al.Miyajima maturation offetal hepatocytes in vitro by extracellular matrices and oncostatin M:induction of tryptophan oxygenase[J].HEPATOLOLY, 2002, 35∶1351-1359.

[11]张淑莲, 曾平鲁, 肖荣, 等.肝干细胞分离培养条件的筛选[J].肝脏, 2003, 8 (1) ∶31-32.

施引文献

资源附件(0)

访问统计