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The relationship between CD4+CD25+ regulatory T Cells and T lymphocytes immunity in tumor microenvironment of hepatocellular carcinoma.
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摘要: 目的了解肝细胞癌组织CD4+CD25+调节性T细胞（以下简写Treg）与肿瘤微环境T细胞免疫的关系。方法对52例肝癌组织和癌旁组织用CD4、CD25双重酶标免疫组化染色和用CD8En Vision法染色,对癌组织中Treg细胞和CD4+T、CD8+T、CD4+T/CD8+T比值进行相关性分析。结果肝癌以及癌旁组织中Treg细胞单个高倍视野平均数分别为7.6308±2.8368、5.1654±1.6718;两组比较有显著差异,P=0.000;肝癌组织中Treg细胞的数量与其浸润性CD4+T淋巴细胞的数量以及CD4+T/CD8+T比值呈显著负相关,r=-0.538,P=0.014;r=-0.545,P=0.000,而与浸润性CD8+T淋巴细胞的数量分布无明显相关性,r=-0.403,P=0.078。结论Treg在肝癌微环境中可能通过细胞接触的方式抑制CD4+T淋巴细胞的增殖来抑制肿瘤局部免疫,使肿瘤细胞逃避免疫监视。因此除去或减少肝癌微环境中的Treg细胞有利于提高肿瘤的免疫治疗效果。
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关键词:
- 肝肿瘤 /
- CD4+CD25+调节性T细胞 /
- 免疫抑制
Abstract: Objective To investigate the relationship between CD4+CD25+ regulatory T cells and T lymphocytes immunity in tumor microenvironment.Methods we marked CD4+CD25+ Treg and CD4+ T cells in the slides tissues using Double-immunohistochemical, and marked CD8+ Tcells in the slides tissues using En Vision.Results The number of Treg cell in the tissues of HCC was more than that of in the tissue of peri-cancer, with significant statistical difference (P=0.000) .The ratio of CD4+T/CD8+T and CD4+ T cell infiltration was positive relation to the number of Treg cell in HCC tissues (r=-0.539, P=0.014, r=-0.545, P=0.000) ;however, CD8+T cell infiltration was no relation to the number of Treg cell in HCC tissues (r=-0.403, P=0.078) .Conclusion Treg cells is the main immunosupression cell in tumor microenvironment of HCC, Treg cells suppress proliferation of CD4+ T cell in a contact-dependent manner possibly so that tumor cells can escape immune surveillance and result in invasion and progression.Functional deletion of tumor-infiltrating Treg cells could enhance tumor-specific immunotherapy.-
Key words:
- hepatocellular carcionma /
- CD4+CD25+ regulatory T cell /
- immunosupression
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[1]Sakaguchi S, Saksguchi N, Asano M, et al.Immunologic self-tol-erance maitainted by activated Tcells expressing IL-2 receptorα-chains (CD25) , Breakdown of single mechanisrn of self-tolerancecauses various autoimmune diseases[J].J Immunol, 1995, 160∶1151-1164. [2]Ohwada S, Iino Y, Nakamura S, et al.Peripheral blood Tcell sub-set as a prognostic factor in gastric cancer[J].Jpn J Clin Oncol, 1994, 24 (1) ∶7-11. [3]傅志旋, 陈晓耕, 刘振华.肿瘤浸润淋巴细胞的抗肿瘤免疫困境[J].国外医学.肿瘤学分册, 2004, 31 (3) ∶183. [4]Kono K, Takahashi A, Ichihara F, et al.Prognostic significance ofadoptive immunotherapy with tumor-associated lymphocytes in pa-tients with advanced gastric cancer.A randomized trial[J].Clin.Cancer Res, 2002, 8∶1767. [5]Kono K, Takahashi A, Sugai H, et al.Dendritic cells pulsed withHER-2/neu-derived peptides can induce specific Tcell responsesin patients with gastric cancer[J].Clin Cancer Res, 2002, 8∶3394. -
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