慢性乙型肝炎病毒感染S区基因缺失及相关因素研究

李鹏; 张大可; 周莉; 董培玲; 李秀惠; 姚勤伟; 王淑珍; 丁惠国; 曾长青

慢性乙型肝炎病毒感染S区基因缺失及相关因素研究

1. 首都医科大学附属北京佑安医院肝病消化科
2. 中国科学院北京基因组研究所

基金项目:

北京市教育委员会基金项目(KM20070025024)；北京市中西医结合重点学科资助；

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中图分类号: R512.6
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出版历程
- 出版日期: 2008-03-20

Prevalences of S region deletion and its related factors in patients with chronic HBV infection

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摘要

摘要: 目的测定慢性乙型肝炎病毒（HBV）感染者HBV DNA全序列,分析S区基因缺失模式、频率及相关因素。方法慢性HBV感染者59例,其中HBV携带7例,慢性肝炎31例,肝硬化10例,重型肝炎6例,原发性肝癌5例。结果25.4%（15/59）慢性HBV感染者有S区基因缺失,未发现S基因缺失。Pre-S基因缺失均见于C基因型患者。Pre-S基因缺失患者中,20%（3/15）HBsAg、抗HBs共存,与无S区缺失者比较有明显差异（P<0.05）。PreS基因缺失与病程（偏相关系数0.28,P=0.049）、抗病毒治疗（偏相关系数-0.451,P=0.036）有密切关系。结论Pre-S基因缺失在基因C型、严重肝病及活动性HBV复制患者多见,可能与病程长及抗病毒治疗有关。Pre-S基因缺失可导致HBV免疫逃避或免疫治疗失败,可能是肝脏疾病发展的重要原因。
- 乙型肝炎病毒 /
- 基因型 /
- 前S基因缺失 /
- 慢性肝病
Abstract: Objective Hepatitis B virus (HBV) mutants with deletions in the S region have not well been evaluated.This study was to assay full-length of HBV DNA, to analyze the pattern, frequency and clinical factors of S region deletion of patients with chronic HBV infection.Methods Full-length sequences of HBV DNA were acquired in 59 patients who included 7 of HBV carrier, 31 of chronic hepatitis, 10 of liver cirrhosis, 6 of chronic severe hepatitis and 5 of primary carcinoma of liver.Genotype of HBV was analyzed with PCR-RFLP.Serum HBV DNA was quantitatively measures with teal-time PCR.The full genome of complete genome of HBV DNA was amplified with routine PCR and fulllength sequencing was directly performed.HBV DNA nucleotide and amino acid sequences were analyzed with bioinformatics technique analysis.Results 25.4% (15/59) of patients with chronic HBV infection had S region deletion.Pre-S gene deletion had a closedly related to genotype C.20% (3/15) patients with Pre-S gene deletion coexisted HBsAg and anti-HBs.There was significant difference compared with patients without Pre-S gene deletion (P<0.05) .Long HBV infection (coefficien 0.28, P=0.049) and antiviral therapy (coefficient-0.451, P=0.036) .Conclusion A greater frequency of Pre-S gene deletion mutants with genotype C, advanced liver disease and active viral replication are demonstrated.It is suggested that Pre-S gene deletion would leads to failure of HBV immune evasion or immunotherapy.
- hepatitis B virus /
- genotype /
- Pre-S deletion /
- chronic liver disease

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参考文献(7)

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