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Detection and significance of Helicobacter pyloyi CagA immunoglobulin G in bile of gallstone patients.
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摘要: 目的探讨幽门螺杆菌（Hp）与胆结石形成的相关性,寻找Hp在胆汁中存在的证据,同时对胆汁中细菌检测方法进行研究分析。方法选择36例胆结石患者,其中28例采用胆管穿刺、8例采用内镜下选择性胆管插管抽取胆汁,用酶联免疫吸附法（ELISA）定量检测胆汁中Hp-CagA IgG,与对照组16例进行比较。结果结石组与对照组、伴有和不伴有急性胆管炎的胆结石患者胆汁中Hp-CagA IgG检出的阳性率均有显著性差异（P<0.05）,而术中胆管穿刺与内镜下选择性胆管插管抽取的胆汁中Hp-CagA IgG检出的阳性率无显著性差异（P>0.05）。结论Hp可能存在于胆道,并与胆结石的形成有一定的关系;经内镜选择性胆管插管抽取胆汁进行Hp-CagA IgG的检测是可行的。
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关键词:
- 胆结石 /
- 幽门螺杆菌 /
- Hp-CagAIgG
Abstract: Objective To study the relationship between the Helicobacter pylori infection and the formation of gallstones.To explore the evidence of Hp existing and the detective methods of bacteria in biliary tract.Methods Twenty-eight cases of patients with gallstone collected bile by direct puncture during operation and other eight cases with gallstone by ERCP operation.Hp CagA immunoglobulin G detected by enzyme-linked immunosorbent assays (ELISA) .Sixteen cases of patients without gallstone was the control group.Results The detection of Hp-CagA IgG of the two groups has significant difference.There was also significant difference with/without acute cholangitis in biliary tract of the patients with gallstone.However, there was no significant difference between the direct puncture group and ERCP group.Conclusion Hp exists possibly in biliary tract and has some relationship with the formation of gallstone.It's feasible to detect Helicobacter pylori CagA IgG through ERCP.-
Key words:
- Gallstone /
- Helicobacter /
- Hp-CagA IgG
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