奥曲肽对肝星状细胞胶原合成及TIMP-1、MMP-2表达的影响

戴永美; 蔡立勉; 林棱

奥曲肽对肝星状细胞胶原合成及TIMP-1、MMP-2表达的影响

基金项目:

基金项目:福建省科技厅(9911)；

详细信息

中图分类号: R575.2
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出版历程
- 出版日期: 2008-05-20

The effect of octreotide on the expression of collagen、TIMP-1 and MMP-2 in HSC1

Research funding:

摘要

摘要: 目的研究奥曲肽（OCT）对体外培养的大鼠肝星状细胞（HSC）胶原分泌及TIMP-1mRNA、MMP-2mRNA表达的影响。方法体外培养大鼠HSC,HSC随机分为4组,分别加入不同浓度的OCT,用ELISA法检测细胞上清液中的Ⅰ、Ⅲ型胶原的含量,半定量RT-PCR法检测OCT对HSC中MMP-2、TIMP-1 mRNA表达的影响。结果应用OCT干预后,HSC合成Ⅰ、Ⅲ型胶原减少,TIMP-1 mRNA的表达较少,而MMP-2 mRNA表达明显增加,差异有显著性（P<0.05）。结论OCT可以抑制HSC的Ⅰ、Ⅲ型胶原的合成,增强MMP-2mRNA的表达,抑制TIMP-1mRNA的表达,这可能是OCT发挥抗肝纤维化作用的途径之一。
- 奥曲肽 /
- 肝星状细胞 /
- 胶原 /
- 基质金属蛋白酶-2基质金属蛋白酶组织抑制因子-1
Abstract: Objective To investigate the effect of octreotide on the expression of collagen、TIMP-1 and MMP-2 in cultured rat hepatic stellate cell (HSC) in vitro.Methods HSC cultured in vitro were randomly divided into 4 groups and different concentrations of octreotide were added to the cell groups.The synthesis of collagen type Ⅰ and type Ⅲ was measured by enzyme-linked immunoaddsordent assay (ELISA) , the expression of TIMP-1 and MMP-2 was detected by semi-quantitative RT-PCR.Results Octreotide remarkably reduced the production of collagen type Ⅰ and type Ⅲ.Octreotide down-regulated the expression of TIMP-1 mRNA, while up-regulated the expression of MMP-2 mRNA. (all P<0.05) .Conclusion Octreotide could significantly reduced the content of collagen type Ⅰ and typeⅢ.Octreotide can promot the expression of TIMP-1 mRNA, while inhibite the expression of MMP-2 mRNA.All of these biological effects of octreotide on HSC were associated with recovery from liver fibrosis.
- Octreotide /
- Hepatic stellate cell /
- Collagen /
- TIMP-1

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参考文献(5)

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