汉民族XRCC1基因399位密码子单核苷酸多态性与原发肝癌关系的研究

任翊; 汪得胜; 李卓; 辛咏梅; 殷继明; 张斌; 丁惠国; 李宁

汉民族XRCC1基因399位密码子单核苷酸多态性与原发肝癌关系的研究

1. 首都医科大学附属北京佑安医院
2. 北京市卫生局肝炎研究所

详细信息

中图分类号: R735.7
计量
- 文章访问数: 2601
- HTML全文浏览量: 8
- PDF下载量: 771
- 被引次数: 0
出版历程
- 出版日期: 2008-05-20

Study on the Relationship between Gene XRCC1 Codon 399 Single Nucleotide Polymorphisms and Primary Hepatic Carcinoma in Han Nationality

摘要

摘要: 目的探讨汉民族XRCC1基因399位密码子单核苷酸多态性与原发肝癌之间的关系。方法原发肝癌患者50例,肝炎肝硬化患者61例,健康献血人员92例。针对XRCC1基因的10号外显子设计引物,PCR产物利用MspⅠ限制性酶切进行基因分型,基因型分成XRCC1 399Arg/Arg,399Arg/Gln,399Gln/Gln三种,分析XRCC1多态性与肝癌之间的关系。结果原发肝癌患者中XRCC1 399Arg/Arg 32例（64.0%）,Arg/Gln 14例（28.0%）,Gln/Gln 4例（8.0%）;肝炎肝硬化患者中Arg/Arg 30例（49.2%）,Arg/Gln 23例（37.7%）,Gln/Gln 8例（13.1%）;健康人群Arg/Arg 46例（50.0%）,Arg/Gln 41例（44.5%）,Gln/Gln 5例（5.5%）。以健康人群为对照组,XRCC1399Gln基因（基因型Arg/Gln和Gln/Gln）并不增加患肝癌的风险性（OR=0.563,95%CI:0.277-1.141,P=0.109）;以肝炎肝硬化为对照组,XRCC1 399Gln基因同样与患肝癌的...
- XRCC1 /
- 基因型 /
- 单核苷酸多态性 /
- 原发肝癌
Abstract: Objective To investigate the relationship between gene XRCC1 codon 399 single nucleotide polymorphisms and primary hepatic carcinoma.Methods We studied 50 primary hepatic carcinoma patients and 61 hepatocirrhosis patients from Beijing You'an hospital.Other 92 healthy blood donors from Beijing area were also included as normal controls.PCR primers were designed according to the exon 10 of gene XRCC1.PCR products including codon 399 single nucleotide polymorphisms were enzymed by MspⅠ.Clinical samples were divided into three genotypes including 399 Arg/Arg, 399 Arg/Gln, and 399 Gln/Gln.The relationship between different genotypes and primary hepatic carcinoma was estimated by statistic method using SPSS software.Results The genotypes of gene XRCC1 codon 399 in 50 primary hepatic carcinoma patients were distributed as follows, Arg/Arg 32 cases (64.0%) , Arg/Gln 14 cases (28.0%) , and Gln/Gln 4 cases (8.0%) .The genotypes in 61 hepatocirrhosis patients were Arg/Arg 30 cases (49.2%) , Arg/Gln 23 cases (37.7%) , and Gln/Gln 8 cases (13.1%) .The genotypes in 92 healthy controls were Arg/Arg 46 cases (50.0%) , Arg/Gln 41 cases (44.5%) , and Gln/Gln 5 cases (5.5%) .Compared to normal controls, XRCC1 399 Gln allele (genotype Arg/Gln and Gln/Gln) did not increase the risk of primary hepatic carcinoma (OR=0.563, 95%CI: 0.277-1.141, P=0.109) .Controlled by hepatocirrhosis group, there was no statistic relationship between 399 Gln allele and primary hepatic carcinoma either (OR=0.544, 95%CI:0.253-1.170, P=0.118) .Conclusion There is no statistic relationship between gene XRCC1 codon 399 single nucleotide polymorphisms and primary hepatic carcinoma in Han nationality in our study.
- XRCC1 /
- genotype /
- single nucleotide polymorphisms /
- primary hepatic carcinoma

HTML全文

参考文献(16)

[1]Charames GS, Bapat B.Genomic instability and cancer[J].CurrMol Med, 2003, 3 (7) ∶589-596.

[2]Ankathil R, Jyothish B, Madhavan J, et al.Deficient DNA repaircapacity:a predisposing factor and high risk predictive marker in fa-milial colorectal cancer[J].J Exp Clin Cancer Res, 1999, 18 (1) ∶33-37.

[3]Goode EL, Ulrich CM, Potter JD.Polymorphisms in DNA repairgenes and associations with cancer risk[J].Cancer Epidemiol Bio-markers Prev, 2002, 11 (12) ∶1513-1530.

[4] Moullan N, Cox DG, Ang埁le S, et al.Polymorphisms in the DNArepair gene XRCC1, breast cancer risk, and response toradiotherapy[J].Cancer Epidemiol Biomarkers Prev, 2003, 12 (11Pt1) ∶1168-1174.

[5]Yeh CC, Sung FC, Rang R, et al.Polymorphisms of the XRCC1, XRCC3, &XPD genes, and colorectal cancer risk:a case-controlstudy in Taiwan[J].BMC Cancer, 2005, 28 (5) ∶12.

[6]DianovaⅡ, Sleeth KM, Allinson SL, et al.XRCC1-DNA poly-merase beta interaction is required for efficient base excisionrepair[J].Nucleic Acids Res, 2004, 32 (8) ∶2550-2555.

[7]Beernink PT, Hwang M, Ramirez M, et al.Specificity of protein in-teractions mediated by BRCTdomains of the XRCC1 DNArepair pro-tein[J].J Biol Chem, 2005, 280 (34) ∶30206-30213.

[8]Caldecott KW.XRCC1 and DNA strand break repair[J].DNA Re-pair, 2003, 2 (9) ∶955-969.

[9]Ladiges W, Wiley J, MacAuley A.Polymorphisms in the DNArepairgene XRCC1 and agerelated diseases[J].Mech Ageing Dev, 2003, 124 (1) ∶27-32.

[10]Abdel-Rahman SZ, Soliman AS, Bondy ML, et al.Inheritance ofthe 194Trp and the 399Gln variant alleles of the DNA repair geneXRCC1 are associated with increased risk of early-onset colorectalcarcinoma in Egypt[J].Cancer Lett, 2000, 159 (1) ∶79-86.

[11]Mort R, Mol L, McEwan C, et al.Lack of involvement of nucleo-tide excision repair gene polymorphisms in colorectal cancer[J].Br JCancer, 2003, 89 (2) ∶333-337.

[12]Yu MW, Yang SY, Pan IJ, et al.Polymorphisms in XRCC1 andglutathione S-transferase genes and hepatitis B-related hepatocel-lular carcinoma[J].J Natl Cancer Inst, 2003, 95 (19) ∶1485-1488.

[13]Long XD, Ma Y, Wei YP, et al.The polymorphisms of GSTM1, GSTT1, HYL1*2, and XRCC1, and aflatoxin B1-related hepato-cellular carcinoma in Guangxi population, China[J].Hepatol Res, 2006, 36 (1) ∶48-55.

[14]Lee JM, Lee YC, Yang SY, et al.Genetic polymorphisms ofXRCC1 and risk of the esophageal cancer[J].Int J Cancer, 2001, 95 (4) ∶240-246.

[15]Xing D, Qi J, Miao X, et al.Polymorphisms of DNA repair genesXRCC1 and XPD and their associations with risk of esophageal squa-mous cell carcinoma in a Chinese population[J].Int J Cancer, 2002, 100 (5) ∶600-605.

[16]Hu Z, Ma H, Chen F, et al.XRCC1 polymorphisms and cancerrisk:a meta-analysis of 38 case-control studies[J].Cancer Epi-demiol Biomarkers Prev, 2005, 14 (7) ∶1810-1818.

施引文献

资源附件(0)

访问统计