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Hepatitis C virus NS5A protein stimulates TLR4 expression
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摘要:
目的探讨丙型肝炎病毒非结构蛋白质5A(HCV NS5A)对Toll样受体4(TLR4)表达的影响。方法用含HCV NS5A基因的表达质粒pcNS5A瞬时转染QSG7701细胞,采用免疫细胞化学技术检测HCV NS5A及TLR4蛋白质的表达;采用逆转录聚合酶链反应观察HCV NS5A和TLR4的mRNA表达。结果转染pcNS5A质粒细胞内有HCV NS5A蛋白的表达。转染pcNS5A组TLR4 mRNA及蛋白质表达均较转染pRc/CMV和未转染质粒组明显增强,而后两组细胞TLR4 mRNA和蛋白质表达相似。结论 HCV NS5A可上调TLR4 mRNA和蛋白的表达。
Abstract:Objective To investigate whether the nonstructural protein 5A(NS5A) encoded by the human hepatitis C virus RNA genome could affect the expression of TLR4.Methods HCV NS5A expression plasmid(pCN5A) was transfected into QSG7701 cells using a lipofectin reagent and the HCV NS5A protein was detected using immuno-histochemistry staining.TLR4 mRNA was evaluated by reverse transcriptase polymerase chain reaction(RT-PCR).Results HCV NS5A was detected in the cytoplasm of the QSG7701 cells transfected with pCN5A,the expression of TLR4 mRNA and protein were both up-regulated in the presence of the HCV NS5A protein.Conclusion HCV NS5A stimulates the transcription of TLR4 mRNA and the expression of TLR4 protein.
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Key words:
- hepacivirus /
- viral nonstructural proteins /
- Toll-like receptor 4
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