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Study on expression and significance of Annexin Ⅰ in liver tissues of rats with hepatic fibrosis
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摘要: 目的检测膜联蛋白Ⅰ(AnnexinⅠ)、转化生长因子(TGF)β1、α–平滑肌肌动蛋白(α–SMA)在大鼠肝纤维化组织中的表达,阐明AnnexinⅠ在肝纤维化中的作用机制。方法雄性SD大鼠随机分为健康对照组6只,肝纤维化模型组24只,采用二甲基亚硝胺(DMN)腹腔注射制备肝纤维化模型。肝纤维化模型组2、4、6、8周各6只大鼠门静脉采血检测血清ALT、AST,采用HE及Masson染色观察肝组织学变化,应用反转录聚合酶链反应(RT–PCR)检测肝组织AnnexinⅠ、TGFβ1、α–SMA mRNA表达,Western Blot法检测肝组织AnnexinⅠ表达。结果与对照组比较,肝纤维化模型组血清ALT、AST水平随损伤时间延长逐渐升高,4周达高峰,6周下降,差异有统计学意义(P<0.05);正常肝组织有AnnexinⅠ、TGFβ1、αSMA低表达,2周即升高,4、6周较明显,8周下降,肝纤维化模型组与对照组在2、4、6、8周各时间点差异有统计学意义(P<0.05)。结论 AnnexinⅠ对肝脏可能有保护作用,为临床肝纤维化的防治提供新策略。Abstract: Objective To investigate the expression and significance of AnnexinⅠ, transforming growth factor beta-1 (TGFβ1) and α-Smooth muscle actin (α-SMA) in liver tissues of rats with hepatic fibrosis, and provide evidences for the mechanisms of AnnexinⅠ in hepatic fibrosis.Methods The male SD rats were divided into two groups randomly.The rat were injected with dimethylnitrosamine (DMN) intra-peritoneally to establish the hepatic fibrosis model.The sera were collected from portal vein from 6 animals 2, 4, 6, 8 weeks after injections in hepatic fibrosis group and levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were tested.The Histopathologic changes of the liver were observed with HE and MASSON stain, AnnexinⅠ, TGFβ1, α-SMA mRNA of liver tissues were determined by RT-PCR and Western Blot was used to detect protein expressions of AnnexinⅠ.Results The level of ALT and AST in fibrosis model group increased gradually with prolonged liver injury than that in the control group, peaked at 4 weeks and decreased at 6 weeks, which was significantly different (P<0.05) .AnnexinⅠ, TGFβ1, α-SMA expression in the liver of normal rat, especially at 4 and 6 weeks after injections, and decreased 8 weeks after injections in fibrosis model group was significantly different 2, 4, 6, 8 weeks after injections (P<0.05) .Conclusion These results collectively suggest that Annexin1 may be a protective agent and may be of therapeutic benefit in the treatment of hepatic fibrosis in clinic.
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Key words:
- annexin A1 /
- transforming growth factor beta l /
- actins /
- liver cirrhosis
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[1]Hannon R, Croxtall JD, Getting SJ, et al.Aberrant inflammation and resistance to glucocorticoids in Annexin1-/-mouse[J].FASEB J, 2003, 17 (2) :253-255. [2]Solito E, Romero IA, Marullo S, et al.Annexin 1 binds to U937 monocytic cells and inhibits their adhesion to microvascular endothelium:involvement of the alpha4beta1 integrin[J].Immunol, 2000, 165 (3) :1573-1581. [3]McArthur S, Cristante E, Paterno M, et al.Annexin A1:a central player in the anti-inflammatory and neuroprotective role of microglia[J].Immunol, 2010, 185 (10) :6317-6328. [4]Zhang Z, Huang L, Zhao W, et al.Annexin 1 induced by anti-inflammatory drugs binds to NF-kappaB and inhibits its activation:anticancer effects in vitro and in vivo[J].Cancer Res, 2010, 70 (6) :2379-2388. [5]de Coupade C, Gillet R, Bennoun M, et al.Annexin I expression and phosphorylation are upregulated during liverregeneration and transformation in antithrombin III SV40 large T antigen transgenic mice[J].Hepatology, 2000, 31 (2) :371-380. [6]Kurosu K, Takiguchi Y, Okada O, et al.Identification of annexin 1 as a novel autoantigen in acute exacerbation of idiopathic pulmonary fibrosis[J].J Immunol, 2008, 181 (1) :756-767. -
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