片仔癀治疗石胆酸灌胃诱导胆囊炎豚鼠模型的效果观察

任彩瑗; 任锋; 李俊峰; 丁美; 陈煜; 段钟平; 张桓虎

doi:10.3969/j.issn.1001-5256.2017.05.023

片仔癀治疗石胆酸灌胃诱导胆囊炎豚鼠模型的效果观察

DOI: 10.3969/j.issn.1001-5256.2017.05.023

1. 山西医科大学第二临床医学院消化科
2. 首都医科大学附属北京佑安医院肝病研究所

基金项目:

国家自然科学基金项目(81270532)；北京市自然科学基金项目(7162085)；首都特色临床应用研究(Z121107001012167)；北京市卫生系统高层次卫生技术人才培养计划(2013-3-075)；北京市医院管理局“登峰”人才培养计划(DFL20151601)；北京市属医学科研院所公益发展改革试点项目(京医研2016-2)；

详细信息

中图分类号: R285.5;R-332
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出版历程
- 出版日期: 2017-05-20

Clinical effect of Pien Tze Huang in treatment of guinea pigs with cholecystitis induced by lithocholic acid given by gavage

Department of Gastroenterology, The Second Clinical Medical School of Shanxi Medical University

Research funding:

摘要

摘要:
目的探讨传统中药片仔癀对胆囊炎动物模型的治疗效果。方法将80只豚鼠（雌雄各半）随机分为空白对照组（10只,与模型组相同剂量的PBS灌胃）,胆囊炎模型组（20只,石胆酸灌胃）,片仔癀组（30只,在胆囊炎模型基础上,加用片仔癀灌胃）,熊去氧胆酸（UCDA）组（20只,在胆囊炎模型基础上,加用UCDA）。观察各组豚鼠的症状、胆囊病理损伤及其肝功能[ALT、AST、TBil、DBil、IBil和总胆汁酸（TBA）]变化。计量资料2组间比较采用独立样本t检验,多组间比较采用单因素方差分析,两两比较采用LSD-t检验,P<0.05为差异有统计学意义。结果与模型组相比,片仔癀组豚鼠状态明显较好,毛发较为光亮整齐,精神状态较好,饮食较好,15 d后均未出现死亡。B超和动态血流图显示,与模型组相比,片仔癀组胆囊形态基本恢复至正常水平,胆囊矢状径[（14.92±1.36）mm vs（16.51±0.65）mm]、横状径[（21.25±1.61）mm vs（23.93±0.84）mm]和胆囊厚度[（0.75±0.049）mm vs（0.90±0.03）mm]均减小,差异均有统计学意义（P值均<0...
- 胆囊炎 /
- 片仔癀 /
- 中成药 /
- 豚鼠 /
- 治疗结果
Abstract:
Objective To investigate the clinical effect of traditional Chinese medicine Pien Tze Huang in the treatment of guinea pigs with cholecystitis.Methods A total of 80 guinea pigs (40 males and 40 females) were randomly divided into blank control group (10 guinea pigs treated with PBS at the same dose as that in model group) , cholecystitis model group (20 guinea pigs treated with lithocholic acid given by gavage) , Pien Tze Huang group (30 guinea pigs treated with Pien Tze Huang by gavage in addition to the treatment in the cholecystitis model group) , and ursodeoxycholic acid group (20 guinea pigs treated with ursodeoxycholic acid in addition to the treatment in the cholecystitis model group) .All groups were observed in terms of symptoms, pathological damage of the gallbladder, and liver function [the levels of alanine aminotransferase (ALT) , aspartate aminotransferase (AST) , total bilirubin (TBil) , direct bilirubin (DBil) , indirect bilirubin (IBil) , and total bile acid (TBA) ].The two-independent-samples t test was used for comparison of continuous data between groups;a one-way analysis of variance was used for comparison between multiple groups and the least significant difference t-test was used for comparison between any two groups.P<0.05 was considered statistically significant.Results Compared with the model group, the Pien Tze Huang group had significantly better conditions with smooth hair, a good mental state, and good food consumption, and no guinea pigs died after 15 days.As was shown by ultrasound and rheography, compared with the model group, the Pien Tze Huang group had basically normal morphology of the gallbladder and significant reductions in sagittal diameter of the gallbladder (14.92±1.36 mm vs 16.51±0.65 mm, P<0.05) , transverse diameter of the gallbladder (21.25±1.61 mm vs 23.93±0.84 mm, P<0.05) , and gallbladder wall thickness (0.75± 0.049 mm vs 0.90±0.03 mm, P<0.05) .HE staining showed that Pien Tze Huang significantly improved the pathological damage of the gallbladder.Compared with the model group, the Pien Tze Huang group had significant reductions in ALT (316.50±264.50 U/L vs684.71±87.73 U/L, P<0.05) , AST (88.78±16.69 U/L vs 180.2±84.40 U/L, P<0.05) , TBil (0.18±0.10) μmol/L vs 1.43 ±0.90 μmol/L, P<0.05) , DBil (0.06±0.05 μmol/L vs 0.23±0.07 μmol/L, P<0.05) , IBil (0.12±0.06 μmol/L vs 1.20±0.55μmol/L, P<0.05) , and TBA (42.40±40.57 μmol/L vs 271.42±148.94 μmol/L, P<0.05) .Conclusion Pien Tze Huang can significantly improve the symptoms, pathological damage of the gallbladder, and liver function in guinea pigs with cholecystitis.
- cholecystitis /
- Pien Tze Huang /
- Chinese patent drugs /
- guinea pigs /
- treatment outcome

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参考文献(16)

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