八宝丹对急性肝衰竭大鼠肝性脑病的防治作用

陆璐; 武超; 谢咚; 安泳潼; 赵亚红; 叶冠; 孙明瑜

doi:10.3969/j.issn.1001-5256.2018.12.025

八宝丹对急性肝衰竭大鼠肝性脑病的防治作用

DOI: 10.3969/j.issn.1001-5256.2018.12.025

1. 上海中医药大学附属曙光医院上海中医药大学肝病研究所肝肾疾病病证教育部重点实验室
2. 上海中医药大学
3. 上海医药集团股份有限公司中央研究院

基金项目:

上海市科委专项(15DZ1900104)；国家自然科学基金项目(81273729)；国家中医药管理局第四批全国中医优秀人才研修项目(2017)；

详细信息

中图分类号: R285.5
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出版历程
- 出版日期: 2018-12-20

Preventive and therapeutic effects of Babaodan on hepatic encephalopathy in rats with acute liver failure

Research funding:

摘要

摘要:
目的探讨八宝丹对急性肝衰竭大鼠肝性脑病的防治作用及机理。方法将50只Wistar大鼠随机分为5组,即正常组、模型组、八宝丹低剂量组、八宝丹高剂量组和乳果糖组。各治疗组每天予以相应药物灌胃1次,正常组和模型组予以同体积0. 3%的羧甲基纤维素钠;灌胃第4天,以硫代乙酰胺（350 mg/kg）腹腔注射诱导肝性脑病,正常组注射等体积生理盐水,每24 h注射1次,共注射3次进行造模,造模后12 h,处死大鼠。HE染色观察肝和脑组织的病理变化,试剂盒检测血氨和肝功能指标,RT-PCR法检测肝组织和脑组织的基因变化。计量资料多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验。结果与模型组比较,八宝丹低剂量组和高剂量组的血氨、TBil、TBA、ALT和AST水平均明显降低（P值均<0. 05）,TP水平明显升高（P值均<0. 05）,八宝丹高剂量组的Alb水平明显升高（P <0. 05）。八宝丹低剂量组和高剂量组肝组织的坏死、炎症均显著改善,脑组织的坏死、核固缩减轻。与模型组相比,八宝丹低剂量组和高剂量组肝组织的TNFα、IL-1、IL-6、NF-κB、Bax、Casp...
- 肝性脑病 /
- 肝功能衰竭,急性 /
- 八宝丹 /
- 大鼠,Wistar
Abstract:
Objective To investigate the preventive and therapeutic effects of Babaodan on hepatic encephalopathy in rats with acute liver failure and possible mechanisms. Methods A total of 50 Wistar rats were randomly divided into normal group, model group, low-dose Babaodan group, high-dose Babaodan group, and lactulose group. The rats in the low-dose Babaodan group, the high-dose Babaodan group, and the lactulose group were given the corresponding drugs by gavage once a day, and those in the normal group and the model group were given an equal volume of 0. 3% sodium carboxymethyl cellulose. On day 4 of gavage, intraperitoneal injection of thioacetamide (350 mg/kg) was performed to induce hepatic encephalopathy, and the rats in the normal group were injected with an equal volume of normal saline, once every 24 hours for 3 times. The rats were sacrificed at 12 hours after the last injection. HE staining was used to observe pathological changes of the liver and the brain; related kits were used to measure blood ammonia and liver function parameters; RT-PCR was used to measure the changes in related genes in liver and brain tissues. The one-way analysis of variance was used for comparison between multiple groups, and the least significant difference t-test was used for further comparison between two groups. Results Compared with the model group, the low-and high-dose Babaodan groups had significant reductions in the levels of blood ammonia, total bilirubin, total bile acid, alanine aminotransferase, and aspartate aminotransferase (all P < 0. 05) and a significant increase in total protein level (P < 0. 05) , and the high-dose Babaodan group had a significant increase in albumin (P < 0. 05) . Both the low-and high-dose Babaodan groups had significant improvements in liver necrosis and inflammation and brain necrosis and karyopyknosis. Compared with the model group, the low-and high-dose Babaodan groups had significant reductions in the mRNA expression of tumor necrosis factor-α (TNF-α) , interleukin-1 (IL-1) , interleukin-6, nuclear factor-kappa B, Bax, caspase-3, and caspase-8 in liver tissue (all P < 0. 05) , and the high-dose Babaodan group had a significant increase in the mRNA expression of Bcl-2 (P < 0. 05) ; the low-and high-dose Babaodan groups also had significant reductions in the mRNA expression of IL-1, TNF-α, inducible nitric oxide synthase, and glutamine synthetase in brain tissue (all P < 0. 05) . Conclusion Babaodan can effectively regulate the expression of inflammatory factors, inhibit the apoptosis and necrosis of hepatocytes, and reduce the production of blood ammonia and inflammatory infiltration, thus alleviating brain injury and exerting therapeutic effect on hepatic encephalopathy in rats with acute liver failure.
- hepatic encephalopathy /
- liver failure, acute /
- Babaodan /
- rats, Wistar

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