人成体肝源性干细胞对酒精性脂肪肝小鼠模型的防治作用

毕研贞; 张秋生; 樊增; 杨永红; 张小蓓; 王全全; 王全义; 王一波; 段钟平; 陈煜; 舒振锋; 司传平; 洪丰

doi:10.3969/j.issn.1001-5256.2019.03.027

人成体肝源性干细胞对酒精性脂肪肝小鼠模型的防治作用

DOI: 10.3969/j.issn.1001-5256.2019.03.027

1. 首都医科大学附属北京佑安医院疑难肝病及人工肝中心,肝衰竭及人工肝治疗研究北京市重点实验室
2. 济宁医学院附属医院消化内科
3. 济宁医学院免疫学与分子医学研究所
4. 济宁医学院附属医院肝病研究所
5. 济宁医学院附属医院神经内科
6. 济宁医学院附属医院病理科

基金项目:

国家科技重大专项“艾滋病和病毒性肝炎等重大传染病防治”(2017ZX10203201-005,2017ZX10202203-006-001,2017ZX10302201-004-002,2012ZX10002004-006,2017ZX10201201)；国家重点研发计划(2017YFA0103000)；国家自然科学基金(81170395,81570556)；

详细信息

中图分类号: R575.5;R-332
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出版历程
- 出版日期: 2019-03-20

Effect of human liver-derived stem cells in prevention and treatment of alcoholic fatty liver disease in mice

Intractable Hepatic Diseases and Artificial Liver Treatment & Training Center, Beijing Key Laboratory of Liver Failure and Atrificial Liver Treatment and Research, Beijing YouAn Hospital, Capital Medical University

Research funding:

摘要

摘要:
目的探讨人肝源性干细胞腹腔移植对小鼠酒精性脂肪肝的防治作用。方法将30只雄性C57BL/6小鼠随机分为空白对照组（N组）、模型对照组（M组）以及肝干细胞移植组（S组）。N组以基础饲料喂养,M组和S组以Lieber-DeCarli酒精液体饲料喂养,S组每周进行2次腹腔肝干细胞移植。干预6周后,测定各组小鼠体质量、肝脏指数,检测血清ALT、AST、TBil、TC、TG、HDL-C、LDL-C,检测肝脏中TG和游离脂肪酸（NEFA）水平,并做肝脏病理学检查和肝组织油红O染色。计量资料多组间比较采用单因素方差分析,进一步两两比较采用SNK-q检验。结果 3组间比较,小鼠血清ALT、AST、TG、TC、HDL-C水平和肝组织TG、NEFA水平差异均具有统计学意义（F值分别为66. 94、7. 15、8. 02、18. 64、3. 86、23. 14、30. 49,P值均<0. 05）。与N组对比,M组小鼠的血清中ALT、AST、TG和肝组织中TG、NEFA明显升高,差异均有统计学意义（P值均<0. 05）。S组小鼠的血清ALT、AST、TG和肝组织TG、NEFA都明显低于M组,差异均...
- 脂肪肝,酒精性 /
- 干细胞移植 /
- 小鼠,近交C57BL
Abstract:
Objective To investigate the effect of intraperitoneal transplantation of human liver-derived stem cells in the prevention and treatment of alcoholic fatty liver disease in mice. Methods A total of 30 male C57 BL/6 mice were randomly divided into blank control group ( group N) , model control group ( group M) , and stem cell transplantation group ( group S) . The mice in group N were fed a normal diet, and those in the other two groups were fed Lieber-DeCarli alcohol liquid diet; at the same time, the mice in group S were given intraperitoneal transplantation of human liver-derived stem cells twice a week. After six weeks of intervention, body weight and liver index were measured, and the serum levels of alanine aminotransferase ( ALT) , aspartate aminotransferase ( AST) , total bilirubin ( TBil) , total cholesterol ( TC) , triglyceride ( TG) , high-density lipoprotein cholesterol ( HDL-C) , and low-density lipoprotein cholesterol ( LDL-C) were also measured. The levels of TG and non-esterified fatty acid ( NEFA) in the liver were measured, and liver pathological examination and oil red O staining of the liver were performed. One-way ANOVA was used for comparison of continuous data between multiple groups, and the SNK-q test was used for further comparison between two groups. Results There were significant differences in the serum levels of ALT, AST, TG, TC, and HDL-C and the content of TG and NEFA in the liver between the three groups ( F = 66. 94, 7. 15, 8. 02, 18. 64, 3. 86, 23. 14 and 30. 49, all P < 0. 05) , Compared with group N, group M showed significant increases in levels of ALT, AST, and TG in serum and levels of TG and NEFA in liver tissue ( all P < 0. 05) . Group S had significantly lower levels of ALT, AST, and TG in serum and levels of TG and NEFA in liver tissue than group M ( all P < 0. 05) . Liver HE staining and oil red O staining showed that group S had a significantly lower degree of liver steatosis than group M. Conclusion Intraperitoneal transplantation of human liver-derived stem cells has a marked effect in the prevention and treatment of alcoholic fatty liver disease in mice.
- fatty liver, alcoholic /
- stem cell transplantation /
- mice, inbred C57BL

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