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Association of vitamin D-related gene polymorphisms with hepatitis B virus-related acute-on-chronic liver failure
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摘要:
目的探究维生素D相关基因单核苷酸多态性（SNP）在HBV相关慢加急性肝衰竭（ACLF）患者中的表达情况及其与HBV-ACLF短期预后的关系。方法纳入福建医科大学附属第一医院肝病中心于2012年7月-2018年3月收治的慢性乙型肝炎患者400例,根据其病程中是否发生ACLF,分为HBV-ACLF组（n=122）和Non-ACLF组（n=278）。分别收集两组患者的临床资料及实验室检查结果,同时采集外周血标本,应用imLDRTM多重SNP分型试剂盒检测维生素D受体（VDR）基因rs1544410、rs2228570及维生素D结合蛋白（VDBP）基因rs2282679共3个位点的SNP表达分型;并对HBV-ACLF组患者进行随访观察,追踪其3个月的生存情况。对比分析维生素D相关基因SNP与HBV-ACLF易感性及预后的关系。采用Hardy-Weinberg平衡检验评估各组基因样本的群体代表性。对于满足正态分布的计量资料,2组间比较采用t检验;对于不满足正态分布的计量资料,2组间比较采用Mann-Whitney U检验。SNP与HBV-ACLF发生的相对风险度采用多元logistic回归分析。...
Abstract:Objective To investigate the expression of single nucleotide polymorphisms (SNP) of vitamin D-related genes in patients with hepatitis B-related acute-on-chronic liver failure (HBV-ACLF) and its association with short-term prognosis of HBV-ACLF. Methods The patients with chronic hepatitis B who were admitted to Liver Diseases Research Center, The First Affiliated Hospital of Fujian Medical University, from July 2012 to March 2018 were enrolled, and according to the presence or absence of acute-on-chronic liver failure during the course of the disease (n = 400) , they were divided into HBV-ACLF group (n = 122) and non-ACLF group (n = 278) . The clinical data and laboratory examination results were collected; peripheral blood samples were collected, and imLDRTM multiple SNP typing kit was used to determine the genotypes of the SNPs of vitamin D receptor (VDR) rs1544410 and rs2228570 and vitamin D-binding protein (VDBP) rs2282679. The patients in the HBV-ACLF group were followed up for 3 months to observe their survival. The association of vitamin D-related gene SNP with HBV-ACLF susceptibility and prognosis was analyzed. Hardy-Weinberg equilibrium was used to evaluate the representativeness of gene samples. The t-test was used for comparison of normally distributed continuous data between two groups, and the Mann-Whitney U test was used for comparison of non-normally distributed continuous data between two groups. A logistic regression analysis was used to investigate the odds ratio of SNP in the development of HBV-ACLF. Results There were no significant differences in the genotype and allele frequencies of VDR rs1544410 and rs2228570 and VDBP rs2282679 between the HBV-ACLF group and the non-ACLF group (all P > 0. 05) . In addition, there were also no significant differences in the genotype and allele frequencies of VDR rs1544410 and rs2228570 and VDBP rs2282679 between the HBV-ACLF patients who survived and those who died (all P > 0. 05) . Conclusion SNPs of VDR rs1544410 and rs2228570 and VDBP rs2282679 are not associated with the development and short-term prognosis of HBV-ACLF.
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