D-双功能蛋白在大鼠和裸鼠肝细胞癌生长中的作用

路欣; 刘英; 贾蕾; 孔令玉; 刘殿卿

doi:10.3969/j.issn.1001-5256.2019.07.022

D-双功能蛋白在大鼠和裸鼠肝细胞癌生长中的作用

DOI: 10.3969/j.issn.1001-5256.2019.07.022

唐山市妇幼保健院检验科
华北理工大学附属医院
河北医科大学

基金项目:

河北省2018年医学科学研究重点课题计划(20181317)；

详细信息

中图分类号: R735.7
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出版历程
- 收稿日期: 2019-02-20
- 出版日期: 2019-07-20

Role of D-bifunctional protein in promoting the growth of hepatocellular carcinoma in rats and nude mice

Department of Clinical Laboratory, Tangshan Maternal and Child Health Hospital

Research funding:

摘要

摘要: 目的探讨肝细胞癌大鼠模型肝癌组织中D-双功能蛋白（DBP）的表达及DBP诱导裸鼠肿瘤生长情况。方法 22只雄性SD大鼠随机分为2组,正常对照组大鼠8只,腹腔注射2-乙基亚硝胺诱导肝细胞癌模型组大鼠14只,蛋白免疫印迹、免疫组化和逆转录聚合酶链反应检测DBP表达。14只雄性SPF级BALB/c-nu小鼠,随机分为2组,用空质粒和DBP过表达质粒分别转染HepG2细胞,将2组细胞分别注射于裸鼠皮下,空质粒对照组8只,DBP高表达组6只,检测2组裸鼠成瘤的大小。计量资料2组间比较采用t检验。结果肝细胞癌模型组大鼠肝癌组织中DBP的蛋白表达高于正常大鼠肝脏,差异具有统计学意义（1. 10±0. 35vs 0. 67±0. 12,t=-7. 48,P <0. 05）; mRNA表达也高于正常大鼠肝脏,差异具有统计学意义（3. 70±0. 85 vs 1. 17±0. 72,t=-20. 46,P <0. 05）。DBP过表达组的裸鼠肿瘤体积显著大于空质粒组,差异具有统计学差异[（7590. 50±1867. 97） mm3vs （1663. 78±420. 24...
- 癌,肝细胞 /
- D-双功能蛋白 /
- 大鼠,Sprague-Dawley /
- 小鼠,近交BALB C /
- 动物实验
Abstract: Objective To investigate the expression of D-bifunctional protein ( DBP) in hepatocellular carcinoma ( HCC) tissue in rats and the growth of DBP-induced HCC in nude mice. Methods A total of 22 male Sprague-Dawley rats were randomly divided into normal control group with 8 rats and model group with 14 rats treated with intraperitoneally injected diethylnitrosamine to induce HCC, and Western blotting, immunohistochemistry, and RT-PCR were used to measure the expression of DBP. A total of 14 specific pathogen-free male BALB/c-nu mice were randomly divided into two groups. HepG2 cells were transfected with empty plasmid or DBP overexpression plasmid and were then injected subcutaneously into nude mice. There were 8 mice in the empty plasmid control group and 6 mice in the DBP high-expression plasmid group, and tumor size was measured for both groups. The t-test was used for comparison of continuous data between groups. Results The rats with HCC had significantly higher protein and mRNA expression of DBP in liver tissue than normal rats ( protein:1. 10 ± 0. 35 vs 0. 67 ± 0. 12, t =-7. 48, P < 0. 05; mRNA: 3. 70 ± 0. 85 vs 1. 17 ± 0. 72, t =-20. 46, P < 0. 05) . The DBP high-expression plasmid group had a significantly higher tumor volume than the empty plasmid group [ ( 7590. 50 ± 1867. 97) mm3 vs ( 1663. 78 ±420. 24) mm3, t =-39. 78, P < 0. 01]. Conclusion Highly expressed DBP can promote the progression of HCC in rats and thus provides a new target for the treatment of HCC and the research and development of inhibitory drugs.
- carcinoma, hepatocellular /
- D-bifunctional protein /
- rats, Sprague-Dawley /
- mice, inbred BALB C /
- animal experimentation

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