基于癌症基因组图集数据库分析锌指蛋白580 mRNA在肝细胞癌中的表达及意义

毛骏; 李仁礼; 李海; 夏时海; 张文成; 向晓辉

doi:10.3969/j.issn.1001-5256.2019.10.020

基于癌症基因组图集数据库分析锌指蛋白580 mRNA在肝细胞癌中的表达及意义

DOI: 10.3969/j.issn.1001-5256.2019.10.020

1. 武警后勤学院
2. 武警特色医学中心消化病科
3. 天津市肝脏胰腺纤维化与分子诊疗重点实验室

基金项目:

天津市自然科学基金(17JCYBJC26700)；

详细信息

中图分类号: R735.7
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出版历程
- 收稿日期: 2019-07-01
- 出版日期: 2019-10-20

Expression and clinical significance of ZNF580 mRNA in hepatocellular carcinoma: An analysis based on The Cancer Genome Atlas database

The Affiliated Tumor Hospital of Guangxi Medical University

Research funding:

摘要

摘要:
目的研究锌指蛋白580（ZNF580） mRNA在肝细胞癌中的表达以及临床价值,预测ZNF580 mRNA在肝细胞癌发生发展中的作用。方法从癌症基因组图集（TCGA）数据库下载肝细胞癌数据集,获得ZNF580 mRNA基因表达谱和临床信息。利用相关生物信息学方法,分析ZNF580 mRNA在肝细胞癌中表达的情况与临床病理指标的相关性以及对预后的影响。用基因富集化分析预测ZNF580 mRNA在肝细胞癌中调控的可能通路。计量资料2组间比较采用t检验,计数资料组间比较采用χ2检验,并运用Cox比例风险回归模型分析影响患者预后的危险因素,计算风险比（HR）及95%可信区间（95%CI）。结果在TCGA数据库中,ZNF580 mRNA的表达水平在肝癌组织中明显高于癌旁组织（8. 440±0. 705 vs 7. 736±0. 387,P<0. 05）,其表达水平在不同肿瘤分级的患者中差异有统计学意义（t=-2. 068,P <0. 05）。ZNF580 mRNA高表达患者的总体生存期明显低于ZNF580 mRNA低表达患者（χ2=5. 456,P <0. 05）。多因素Co...
- 癌,肝细胞 /
- 锌指蛋白580 mRNA /
- 计算生物学 /
- 危险因素
Abstract:
Objective To establish and validate a nomogram for overall survival(OS) of patients after hepatectomy for hepatocellular carcinoma(HCC). Methods A retrospective analysis was performed for the clinical data of 1013 patients who underwent hepatectomy for HCC in The Affiliated Tumor Hospital of Guangxi Medical University from February 2004 to October 2013. These patients were randomly divided into training cohort with 710 patients and validation cohort with 303 patients. For the training cohort,the Cox proportional hazards model was used to determine independent risk factors and a nomogram was established to predict 1-,3-,and 5-year survival rates. The performance of this nomogram was evaluated by internal verification within the training cohort and external verification of the validation cohort,as well as C-index,receiver operating characteristic(ROC) curve,and calibration curve. The independent samples t-test was used for comparison of continuous variables between groups,and the chi-square test or the Fisher's exact test was used for comparison of categorical variables between groups. The Cox proportional hazards model was used for univariate and multivariate analyses. Results The 1-,3-,and 5-year OS rates in the training cohort were 0. 72,0. 48,and 0. 34,respectively,and those in the validation cohort were 0. 66,0. 45,0. 32,respectively. The univariate and multivariate analyses showed that age,number of tumors,tumor diameter,tumor capsule,vascular invasion,microsatellite lesion,aspartate aminotransferase(AST),and alpha-fetoprotein(AFP) were the influencing factors for OS in patients after hepatectomy for HCC(all P < 0. 05),and such factors were used to establish a nomogram model. In the training cohort,the C-index for predicting OS was 0. 748(95% confidence interval [CI]: 0. 712-0. 784); the calibration curve of 1-,3-,and 5-year survival rates showed that the predicted value of the nomogram was in good consistency with the actual values observed; this nomogram model had an area under the ROC curve of 0. 81(95% CI: 0. 76-0. 87),0. 82(95% CI: 0. 77-0. 88),and 0. 79(95% CI: 0. 71-0. 88),respectively,in predicting the 1-,3-,and 5-year survival rates. In the validation cohort,the C-index was 0. 712(95% CI: 0. 685-0. 739); the calibration curve of 1-,3-,and 5-year survival rates showed that the predicted value of the nomogram was in good consistency with the actual values observed; this nomogram model had an area under the ROC curve of 0. 75(95% CI: 0. 71-0. 79),0. 77(95%CI: 0. 73-0. 81),and 0. 74(95% CI: 0. 68-0. 80),respectively,in predicting the 1-,3-,and 5-year survival rates. Conclusion The nomogram established in this study can effectively predict OS in patients after hepatectomy for HCC.
- carcinoma,hepatocellular /
- prognosis /
- nomogram /
- risk factors

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