干扰素α和胸腺五肽协同干预对HepG2.2.15细胞APOBEC3A和APOBEC3B mRNA表达的影响

熊芳; 高耀; 马艳品; 于乐乐; 谭炳琴; 鲍旭丽; 闾军

doi:10.3969/j.issn.1001-5256.2020.01.017

干扰素α和胸腺五肽协同干预对HepG2.2.15细胞APOBEC3A和APOBEC3B mRNA表达的影响

DOI: 10.3969/j.issn.1001-5256.2020.01.017

首都医科大学附属北京佑安医院肝病与肿瘤生物治疗科

基金项目:

十二五国家科技重大专项任务级课题(2014ZX10002002-001-002)；首都卫生发展科研专项项目(2018-1-2181)；首都临床特色应用研究(Z181100001718191)；北京市属医院科研培育项目(PX2019063)；

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中图分类号: R373.2
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出版历程
- 收稿日期: 2019-08-13
- 出版日期: 2020-01-20

Effect of interferon-α and thymopentin on the mRNA expression of APOBEC3A and APOBEC3B in HepG2.2.15 cells

Department of Hepatopathy and Cancer Biotherapy,Beijing YouAn Hospital,Capital Medical University

Research funding:

摘要

摘要: 目的探讨IFNα和胸腺五肽（TP5）协同干预对HepG2. 2. 15细胞载脂蛋白B mRNA编辑酶催化多肽3A（APOBEC3A）、APOBEC3B基因mRNA表达水平的影响。方法将HepG2. 2. 15细胞分为空白对照组、IFN处理组、TP5处理组和IFNα联合TP5组,在处理的12、24、48和72 h用实时荧光定量PCR方法检测细胞APOBEC3A、APOBEC3B基因mRNA表达水平。计量资料多组间比较采用方差分析,进一步两两比较采用LSD-t检验。结果与空白对照组相比,IFNα、IFNα联合TP5分别处理12、24、48和72 h后,APOBEC3A mRNA的表达水平显著提高,差异均有统计学意义（P值均<0. 001）。与IFNα处理组相比,4个时间点IFNα联合TP5组APOBEC3A mRNA的表达水平显著提高,差异均有统计学意义（P值<0. 001）。TP5处理在各时间点对APOBEC3A mRNA表达水平的影响差异无统计学意义（P值均> 0. 05）。与对照组相比,所有处理组中APOBEC3B的mRNA表达水平差异无统计学意义（P值均> ...
- 干扰素α /
- 胸腺喷丁 /
- APOBEC脱氨酶类
Abstract: Objective To investigate the effect of synergistic intervention of interferonα(IFNα) and thymopentin(TP5) on the mRNA expression of apolipoprotein B mRNA editing enzyme,catalytic polypeptide-like 3 A(APOBEC3 A) and apolipoprotein B mRNA editing enzyme,catalytic polypeptide-like 3 B(APOBEC3 B) in HepG2. 2. 15 cells. Methods HepG2. 2. 15 cells were divided into blank control group,IFNα treatment group,TP5 treatment group,and IFNα + TP5 treatment group,and at 12,24,48,and 72 hours of treatment,quantitative real-time PCR was used to measure the mRNA expression of APOBEC3 A and APOBEC3 B in HepG2. 2. 15 cells. An analysis of variance was used for comparison of continuous data between multiple groups,and the least significant difference t-test was used for further comparison between two groups. Results Compared with the blank control group,the IFNα treatment group and the IFNα + TP5 treatment group had a significant increase in the mRNA expression of APOBEC3 A at 12,24,48,and 72 hours of treatment(all P < 0. 001). Compared with the IFNα treatment group,the IFNα + TP5 treatment group had a significant increase in the mRNA expression of APOBEC3 A at these four time points(all P < 0. 001). TP5 treatment had no significant influence on the mRNA expression of APOBEC3 A at each time point(all P > 0. 05). There was no significant difference in the mRNA expression of APOBEC3 B between the blank control group and the treatment groups(all P > 0. 05). Conclusion IFNα combined with TP5 can significantly upregulate the mRNA expression of APOBEC3 A in HepG2. 2. 15 cells.
- interferon-alpha /
- thymopentin /
- APOBEC deaminases

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