辅助性T淋巴细胞17/调节性T淋巴细胞比值失衡在免疫性肝损伤大鼠模型中的变化特点

薛苗; 毛小荣; 陈红

doi:10.3969/j.issn.1001-5256.2020.01.033

辅助性T淋巴细胞17/调节性T淋巴细胞比值失衡在免疫性肝损伤大鼠模型中的变化特点

DOI: 10.3969/j.issn.1001-5256.2020.01.033

1. 兰州大学第一医院风湿免疫科
2. 兰州大学第一医院感染科

基金项目:

甘肃省自然科学基金(1606RJZA127)；

详细信息

中图分类号: R575;R-332
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出版历程
- 收稿日期: 2019-07-15
- 出版日期: 2020-01-20

Change in T helper 17 cell/regulatory T cell imbalance in rats with immunological liver injury

Department of Rheumatology and Immunology,The First Hospital of Lanzhou University

Research funding:

摘要

摘要: 目的探讨大鼠免疫性肝损伤模型建立的方法以及辅助性T淋巴细胞（Th） 17/调节性T淋巴细胞（Treg）失衡在免疫性肝炎大鼠模型中的变化特点。方法采用随机数字表法将30只雌性Wistar大鼠分为3组,每组10只,分别是急性免疫性肝损伤模型（AC）组、慢性免疫性肝损伤模型（CC）组和健康对照（HC）组。AC组和CC组分别通过尾静脉注射α-galcer和刀豆蛋白A（ConA）建立,HC组注射等量的生理盐水。建模成功后检测血清转氨酶ALT、AST等; HE染色观察大鼠肝脏病理改变;免疫组化法检测肝脏中Foxp3、RORγt蛋白表达。外周血和脾脏悬液中Th17、Treg细胞频率变化采用流式细胞分析术检测并计算Th17/Treg比值。计量资料多组间比较采用单因素方差分析,进一步两两比较用SNK-q检验。结果与HC组比较,AC组、CC组血清中ALT、AST、TBil均升高,Alb水平降低,差异均有统计学意义（F值分别为14. 782、20. 765、16. 088、74. 181,P值均<0. 001）。HE染色,与HC组相比,AC组、CC组分别出现急慢性肝细胞损伤改变。与HC组相比,AC组T...
- 肝损伤 /
- Th17细胞 /
- T淋巴细胞,调节性 /
- 大鼠,Wistar
Abstract: Objective To investigate the methods for establishing a rat model of immunological liver injury and the change in T helper 17 cell(Th17)/regulatory T cell(Treg) imbalance in rats with immunological liver injury. Methods A total of 30 female Wistar rats were divided into acute immunological liver injury group(AC group),chronic immunological liver injury group(CC group),and healthy control group(HC group) using a random number table,with 10 rats in each group. The rats in the AC group and the CC group were given tail vein injection of α-galactosylceramide(α-GalCer) and concanavalin A(ConA) to establish a rat model,and those in the HC group were given injection of an equal volume of normal saline. The serum levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST)were measured after modeling,HE staining was used to observe liver pathological changes,and immunohistochemistry was used to measure the protein expression of Foxp3 and RORγt in the liver. Flow cytometry was used to measure the changes in the frequencies of Th17 and Treg cells in peripheral blood and spleen suspension,and Th17/Treg ratio was calculated. A one-way analysis of variance was used for comparison of continuous data between multiple groups,and the SNK-q test was used for further comparison between two groups. Results Compared with the HC group,the AC group and the CC group had significant increases in the serum levels of ALT,AST,and total bilirubin and a significant reduction in the level of albumin(F = 14. 782,20. 765,16. 088,and 74. 181,all P < 0. 001). HE staining showed that compared with the HC group,the AC group and the CC group had the changes of acute and chronic hepatocyte injury. Compared with HC group,the AC group had significant increases in the frequency of Th17 cells,the protein expression of RORγt(all P < 0. 05),the frequency of Treg cells(P < 0. 05),and Th17/Treg ratio(P < 0. 05). Compared with the HC group,the CC group had significant increases in the frequencies of Th17 and Treg cells and a significant reduction in Th17/Treg ratio(all P < 0. 05). Conclusion Both α-GalCer and ConA can be used to successfully establish a model of immunological liver injury without deaths,and the changes in liver histology and serum biochemistry are consistent with acute and chronic immune hepatitis. Th17/Treg imbalance plays an important role in inflammatory response and tis-sue damage and is closely associated with immune status and disease progression.
- liver injury /
- Th17 cells /
- T-lymphocytes,regulatory /
- rats,Wistar

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