不同阶段慢性乙型肝炎患者外周血单个核细胞中NF-κB/iNOS的表达及免疫状态

黄津; 张美泉; 何道兴

doi:10.3969/j.issn.1001-5256.2020.03.013

不同阶段慢性乙型肝炎患者外周血单个核细胞中NF-κB/iNOS的表达及免疫状态

DOI: 10.3969/j.issn.1001-5256.2020.03.013

1. 福建医科大学附属南平第一医院感染科
2. 福建省立医院北院感染科

基金项目:

福建省卫生计生委青年基金(2017-1-94)；

详细信息

中图分类号: R512.62
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出版历程
- 出版日期: 2020-03-20

Expression of nuclear factor-kappa B and inducible nitric oxide synthase in peripheral blood mononucleated cells and immune status in patients with different stages of chronic hepatitis B

Department of Infectious Diseases, Nanping First Hospital Affiliated to Fujian Medical University

Research funding:

摘要

摘要: 目的研究核因子-κB（NF-κB）/诱导型一氧化氮合成酶（iNOS）在不同阶段慢性乙型肝炎患者外周血单个核细胞（PBMC）中的表达,比较各阶段自然杀伤细胞及T、B淋巴细胞的变化情况。方法纳入福建医科大学附属南平第一医院2017年10月-2019年1月慢性乙型肝炎相关疾病患者,按照疾病不同阶段分4组:肝炎组、肝衰竭组、肝硬化组、肝癌组,每组各20例;另选取10例健康志愿者作为对照组。采用流式细胞仪检测5组外周血总淋巴细胞计数和淋巴细胞亚群计数;采用Western Blot检测PBMC中NF-κB、iNOS蛋白的表达水平;硝酸还原酶法和ELISA法检测血清中NO、IL-6水平。多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验。结果与对照组比较,肝炎组、肝衰竭组、肝癌组、肝硬化组总淋巴细胞计数水平均明显下降（P值均<0. 05）;与肝炎组比较,肝衰竭组、肝癌组、肝硬化组总淋巴细胞计数水平均明显下降（P值均<0. 05）。肝衰竭组、肝癌组、肝硬化组CD3+明显下降,分别与对照组、肝炎组比较差异均有统计学意义（P值均<0. 05）;肝衰竭组、...
- 乙型肝炎,慢性 /
- NF-κB /
- 一氧化氮合酶 /
- 淋巴细胞
Abstract: Objective To investigate the expression of nuclear factor-kappa B( NF-κB) and inducible nitric oxide synthase( iNOS) in peripheral blood mononucleated cells( PBMCs) of patients with different stages of chronic hepatitis B( CHB),as well as the changes in natural killer( NK),T,and B lymphocytes in each stage. Methods A total of 80 patients with CHB-related diseases who were admitted to Nanping First Hospital Affiliated to Fujian Medical University from October 2017 to January 2019 were enrolled and divided into four groups according to disease stage: hepatitis group,liver failure group,liver cirrhosis group,and liver cancer group,with 20 patients in each group,and ten healthy volunteers were enrolled as control group. Flow cytometry was used to measure total lymphocyte count as well as the counts of lymphocyte subsets in peripheral blood in all five groups,Western blot was used to measure the protein expression of NF-κB and iNOS in PBMCs,and the nitrate reductase method and ELISA were used to measure the serum levels of nitric oxide( NO) and interleukin-6( IL-6). A one-way analysis of variance was used for comparison between multiple groups,and the least significant difference t-test was used for further comparison between two groups. Results Compared with the control group,the hepatitis group,the liver failure group,the liver cancer group,and the liver cirrhosis group had a significant reduction in total lymphocyte count( all P < 0. 05),and compared with the hepatitis group,the liver failure group,the liver cancer group,and the liver cirrhosis group had a significant reduction in total lymphocyte count( all P < 0. 05). The liver failure group,the liver cancer group,and the liver cirrhosis group had a significant reduction in CD3+T cells compared with the control group and the hepatitis group( all P < 0. 05),as well as a significant reduction in CD4+T cells compared with the hepatitis group( all P < 0. 05); the liver failure group had a significant increase in CD8+T cells compared with the liver cancer group,the liver cirrhosis group,and the control group( all P < 0. 05); the liver failure group had a significant reduction in CD4+/CD8+compared with the control group,the hepatitis group,the liver cirrhosis group,and the liver cancer group( all P < 0. 05); the liver failure group and the liver cirrhosis group had a significant increase in CD3-CD19+B cells compared with the hepatitis group and the control group( all P < 0.05); the liver failure group and the liver cancer group had a significant reduction in CD16+CD56+NK cells compared with the hepatitis group and the control group( all P < 0. 05). The liver cirrhosis group,the liver failure group,and the liver cancer group had significant increases in the protein expression of NF-κB and iNOS compared with the hepatitis group and the control group( all P < 0. 05). Compared with the control group,the liver failure group,the liver cancer group,the liver cirrhosis group,and the hepatitis group had significant increases in the serum levels of NO and IL-6( all P < 0. 05). Conclusion There are certain differences in immune status between patients with different stages of HBV infection,and NF-κB-iNOS-NO may be involved in the immune response of HBV-related diseases.
- hepatitis B,chronic /
- NF-kappa B /
- nitric oxide synthase /
- lymphocytes

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参考文献(16)

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