雷公藤甲素对雨蛙素诱导的慢性胰腺炎小鼠模型胰腺纤维化的影响

谭鹏; 陈浩; 王安康; 钱保林; 黄治伟; 石昊; 高琳; 付文广

doi:10.3969/j.issn.1001-5256.2020.03.034

雷公藤甲素对雨蛙素诱导的慢性胰腺炎小鼠模型胰腺纤维化的影响

DOI: 10.3969/j.issn.1001-5256.2020.03.034

1. 西南医科大学附属医院四川省院士(专家)工作站
2. 西南医科大学附属医院肝胆外科
3. 西南医科大学附属医院健康管理中心

基金项目:

四川省应用基础研究项目(2018JY0283)；四川省卫生健康委员会科研课题(19PJ292)；

详细信息

中图分类号: R285.5;R-332
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出版历程
- 收稿日期: 2019-11-07
- 出版日期: 2020-03-20

Effect of triptolide on fibrosis in a mouse model of cerulean-induced chronic pancreatitis

Academician Expert Workstation of Sichuan Province,The Affiliated Hospital of Southwest Medical University

Research funding:

摘要

摘要: 目的基于NF-κB/IL-6信号通路研究雷公藤甲素对雨蛙素诱导的慢性胰腺炎（CP）小鼠模型胰腺纤维化的影响。方法随机将15只C57BL/6雄性小鼠分为对照组（生理盐水处理,n=5）、雨蛙素组（雨蛙素诱导CP模型,n=5）和雷公藤甲素组（雨蛙素诱导+雷公藤甲素治疗,n=5）。6周后采集样本进行检测。对小鼠胰腺称重;苏木素-伊红染色、天狼猩红及马松染色观察胰腺组织形态及胶原沉积; ELISA检测血清中IL-6的表达;免疫组化检测α平滑肌肌动蛋白（α-SMA）、NF-κB/p65的表达情况;蛋白质免疫印迹检测NF-κB/p65、IL-6及α-SMA的表达。计量资料多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验。结果 3组间胰腺组织重量,IL-6水平,病理评分,天狼猩红染色、马松染色、α-SMA阳性信号、NF-κB/p65阳性信号的平均光密度值比较差异均有统计学意义（F值分别为64. 87、15. 85、145. 33、141. 80、121. 77、250. 22、69. 22,P值均<0. 001）。与雨蛙素组相比,雷公藤甲素组胰腺重量明显增加,IL-6的表达明显降低...
- 胰腺炎,慢性 /
- 雷公藤属 /
- 雨蛙肽 /
- 小鼠,近交C57BL
Abstract: Objective To investigate the effect of triptolide on fibrosis in mice with cerulein-induced chronic pancreatitis( CP) based on the nuclear factor-kappa B( NF-κB)/interleukin-6( IL-6) signaling pathway. Methods A total of 15 male C57 BL/6 mice were randomly divided into control group( treated with normal saline),cerulein group( model mice with cerulein-induced CP),and triptolide group( induced by cerulean and treated with triptolide),with 5 mice in each group. Samples were collected for detection after 6 weeks. The weight of the pancreas was measured; HE staining,picrosirius red staining,and Masson staining were used to observe pancreatic histomorphology and collagen deposition; ELISA was used to measure the expression of IL-6 in serum; immunohistochemistry was used to measure the expression of alpha-smooth muscle actin( α-SMA) and NF-κB/p65; Western blot was used to measure the expression of NF-κB/p65,IL-6,and α-SMA. A one-way analysis of variance was used for comparison of continuous data between multiple groups,and the least significant difference t-test was used for further comparison between two groups. Results There were significant differences between the three groups in the weight of the pancreas,IL-6 concentration,pathological score,picrosirius red staining results,Masson staining results,and mean optical density of positive α-SMA signal and positive NF-κB/p65 signal( F = 64. 87,15. 85,145. 33,141. 80,121. 77,250. 22,and 69. 22,all P < 0. 001). Compared with the cerulein group,the triptolide group had a significant increase in the weight of the pancreas and significant reductions in the expression of IL-6,pancreatic fibrosis,collagen deposition,and the expression of α-SMA and NF-κB/p65( all P < 0. 05). Western blotting showed that there were significant differences in the expression of NF-κB/p65,IL-6,andα-SMA between the three groups( F = 8. 86,6. 74,and 16. 23,all P < 0. 05),and compared with the cerulein group,the triptolide group had significantly lower expression of NF-κB/p65,IL-6,and α-SMA in the pancreatic tissue( all P < 0. 05). Conclusion Triptolide alleviates fibrosis in mice with cerulein-induced CP and inhibit the protein expression of NF-κB/p65 and IL-6.
- pancreatitis,chronic /
- tripterygium /
- caerulein /
- mice,inbred C57BL

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