黛矾散干预Th17/Treg平衡防治原发性胆汁性胆管炎的作用机制

占凯; 徐燕; 韩梦玲; 程良斌

doi:10.3969/j.issn.1001-5256.2018.11.021

黛矾散干预Th17/Treg平衡防治原发性胆汁性胆管炎的作用机制

DOI: 10.3969/j.issn.1001-5256.2018.11.021

1. 襄阳市中医院肝胆病科
2. 武汉万济精神病医院精神科
3. 广东省中医院大学城医院肝病科
4. 湖北省中医院肝病研究所

基金项目:

湖北省教育厅重点项目(D20112001)；武汉市科技局基金项目(武科技[2015]203号2015061701011646)；

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中图分类号: R575.7
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出版历程
- 出版日期: 2018-11-20

Mechanism of action of Daifan powder in the prevention and treatment of primary biliary cholangitis via its interventional effect on Th17/Treg balance

Research funding:

摘要

摘要:
目的观察不同剂量的黛矾散对聚肌胞苷酸（Poly I∶C）诱导的原发性胆汁性胆管炎（PBC）模型小鼠外周血辅助性T淋巴细胞17（Th17）、CD4+CD25+Foxp3+调节性T淋巴细胞（Treg）及Th17/Treg比值的影响,进一步探讨黛矾散治疗PBC的作用机制。方法将90只C57BL/6雌性小鼠随机分成正常组、模型组、黛矾散低剂量组、黛矾散中剂量组、黛矾散高剂量组、熊去氧胆酸（UDCA）组,用Poly I:C诱导PBC模型,观察黛矾散对PBC模型小鼠血清学的影响。检测外周血中肝功能相关指标,采用流式细胞技术检测外周血Treg、Th17比例,HE染色检测肝组织病理结构。符合正态分布的计量资料2组间比较采用t检验,多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验;非正态分布的计量资料2组间比较采用两独立样本Mann-Whitney U非参数检验,多组间比较采用多独立样本Kraskal-Wallis H非参数检验,相关分析采用Spearman相关性检验。结果与正常组比较,模型组和UDCA组血清ALT、AST、...
- 黛矾散 /
- 肝硬化,胆汁性 /
- T淋巴细胞,辅助诱导 /
- T淋巴细胞,调节性
Abstract:
Objective To investigate the effect of different doses of Daifan powder on peripheral blood T helper 17 ( Th17) cells, CD4+CD25+Foxp3+regulatory T ( Treg) cells, and Th17/Treg ratio in a mouse model of primary biliary cholangitis ( PBC) induced by polyinosinic-polycytidylic acid ( Poly I∶ C) and the mechanism of action of Daifan powder in the treatment of PBC. Methods A total of 90 C57 BL/6 female mice were randomly divided into normal group, model group, low-, middle-, and high-dose Daifan powder groups, and ursodeoxycholic acid ( UDCA) group. Poly I: C was used to establish a model of PBC, and the effect of Daifan powder on the serology of PBC mice was observed. Liver function parameters were measured; flow cytometry was used to measure the percentages of peripheral blood Treg cells and Th17 cells; HE staining was used to observe liver pathology. The t-test was used for comparison of normally distributed continuous data between two groups; a one-way analysis of variance was used for comparison between multiple groups, and the least significant difference t-test was used for further comparison between two groups. The Mann-Whitney U test ( for two independent samples) was used for comparison of non-normally distributed continuous data between two groups; the Kruskal-Wallis H test ( for two or more independent samples) was used for comparison between multiple groups. Spearman correlation was used for the correlation analysis. Results Compared with the normal group, the model group and the UDCA group had significant increases in the serum levels of alanine aminotransferase ( ALT) , aspartate aminotransferase ( AST) , alkaline phosphatase ( ALP) , total bilirubin ( TBil) , and direct bilirubin ( DBil) ( all P <0. 05) . Compared with the model group, the low-, middle-, and high-dose Daifan powder groups had significant reductions in the serum levels of ALT, AST, ALP, TBil, and DBil ( all P < 0. 05) . In the model group, peripheral blood Th17/Treg ratio was not correlated with ALT, AST, ALP, TBil, and DBil ( all P < 0. 05) . Compared with the normal group, the model group had a significant increase in the percentage of peripheral blood Th17 cells ( P < 0. 01) ; compared with the model group, the low-, middle-, and high-dose Daifan powder groups had a significant reduction in the percentage of peripheral blood Th17 cells ( all P < 0. 01) . Compared with the normal group, the model group had a significant reduction in the percentage of peripheral blood Treg cells ( P < 0. 01) ; compared with the model group, the low-, middle-, and high-dose Daifan powder groups had a significant increase in the percentage of peripheral blood Treg cells ( all P <0. 01) . Compared with the normal group, the model group had a significant increase in peripheral blood Th17/Treg ratio ( P < 0. 01) ; compared with the model group, the low-, middle-, and high-dose Daifan powder groups had a significant reduction in peripheral blood Th17/Treg ratio ( all P < 0. 01) . Conclusion Daifan powder exerts a therapeutic effect on PBC by breaking Th17/Treg balance and reducing immune inflammatory reaction.
- Daifansan /
- liver cirrhosis, biliary /
- T-lymphocytes, helper-inducer /
- T-lymphocytes, regulatory

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参考文献(22)

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