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Volume 41 Issue 11
Nov.  2025
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Article Navigation >  Journal of Clinical Hepatology  > 2025  >  41(11): 2265-2271

The mRNA expression of magnesium transporter 1 in CD8+ T cells and its correlation with HBV DNA in patients with chronic HBV infection

DOI: 10.12449/JCH251111
  • 1.

    Department of Hepatobiliary Surgery，Tangshan Workers’Hospital，Tangshan，Hebei 063000，China

  • 2.

    Department of Nuclear Medicine，Tangshan Workers’Hospital，Tangshan，Hebei 063000，China

  • 3.

    Department of Laboratory Medicine，Tangshan Workers’Hospital，Tangshan，Hebei 063000，China

  • 4.

    Department of Clinical Laboratory，Tangshan Eighth Hospital，Tangshan，Hebei 063000，China

Research funding:

Hebei Medical Science Research Project Plan (20250218)

More Information
  • Corresponding author: LIN Xiaoe， linxiao_e1980@163.com （ORCID： 0009-0003-0384-4882）
  • Received Date: 2025-06-22
  • Accepted Date: 2025-08-05
  • Published Date: 2025-11-25
    Abstract
  •   Objective  To investigate the change in the expression of magnesium transporter 1 （MagT1） in peripheral blood CD8+ T cells in patients with chronic hepatitis B virus （HBV） infection， as well as the correlation of serum Mg2+ and MagT1 with HBV DNA load.  Methods  A total of 102 patients with HBV infection who were admitted to Tangshan Workers’ Hospital from January 2022 to December 2023 were enrolled， and a case-control study was conducted. According to the stage of disease progression， the subjects were divided into chronic hepatitis B group with 40 patients， compensated liver cirrhosis group with 32 patients， and hepatocellular carcinoma group with 30 patients， and 32 healthy volunteers matched by age and sex were enrolled as normal control group. The serum concentration of Mg²⁺ was measured； RT-qPCR was used to measure the mRNA expression level of MagT1 in CD8+ T cells and serum HBV DNA load； flow cytometry was used to measure the expression levels of programmed death-1 （PD-1）， T-cell immunoglobulin and mucin-domain containing-3 （Tim-3）， and natural killer cell group 2D （NKG2D） on the surface of CD8+ T cells. A one-way analysis of variance was used for comparison of normally distributed continuous data between multiple groups， and the SNK-q test was used for comparison between two groups； the chi-square test was used for comparison of categorical data between groups； a Pearson linear correlation analysis was used to investigate the correlation between the mRNA expression level of MagT1 and other related indicators.  Results  There were significant differences in the serum level of Mg2+ and the mRNA expression level of MagT1 between the normal control group， the chronic hepatitis B group， the compensated liver cirrhosis group， and the hepatocellular carcinoma group （F=29.014 and 145.578， both P<0.001）. The Pearson correlation analysis showed that the serum level of Mg2+ and the mRNA expression level of MagT1 were positively correlated with HBV DNA load （r=0.335 and 0.394， both P<0.05）. The hepatocellular carcinoma group had the highest levels of PD-1 and Tim-3， followed by the compensated liver cirrhosis group， the chronic hepatitis B group， and the normal control group； the normal control group had the highest level of NKG2D， followed by the chronic hepatitis B group， the compensated liver cirrhosis group， and the hepatocellular carcinoma group （all P<0.001）. The Pearson correlation analysis showed that the mRNA expression level of MagT1 was negatively correlated with PD-1 and Tim-3 （r=-0.643 and -0.640， both P<0.05） and was positively correlated with NKG2D （r=0.655， P<0.05）.  Conclusion  There is a reduction in the mRNA expression level of MagT1 in peripheral blood CD8+ T cells in patients with HBV infection， which is positively correlated with serum HBV DNA load. The reduction in the expression of MagT1 may contribute to CD8+ T cell exhaustion by impairing Mg²⁺ influx， manifesting as the upregulation of PD-1 and Tim-3 and the downregulation of NKG2D.

     

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