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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 9
Sep.  2013
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Determination of serum M30 and M65 levels and its clinical significance in patients with HBV- related acute- on-chronic liver failure

DOI: 10.3969/j.issn.1001-5256.2013.09.011
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  • Received Date: 2013-01-21
  • Published Date: 2013-09-20
  • Objective To determine the serum M30 and M65 levels in patients with HBV- related acute- on- chronic liver failure ( ACLF) and to investigate their association with this disease. Methods Thirty- one patients with HBV- related ACLF ( 20 cases with response to treatment and 11 cases without response to treatment) , 20 patients with chronic hepatitis B ( CHB) , and 10 healthy controls were enrolled in the study. Serum M65 and M30 levels were measured by enzyme- linked immunosorbent assay. Analysis of variance was used for comparison among groups; q- test was used for pairwise comparison. Results The serum M30 and M65 levels of ACLF patients ( 508. 65 ±340. 16 and 768. 75 ± 290. 02 U / L) were significantly higher than those of CHB patients ( 212. 27 ± 91. 33 and 384. 40 ± 134. 46 U / L) and healthy controls ( 94. 12 ± 17. 64 and 121. 99 ± 29. 25 U / L) ( P < 0. 05 for all comparisons) . In the ACLF patients, the serum level of M30was positively correlated with that of M65 ( r = 0. 78, P < 0. 05) ; no significant differences were seen between the cases with response to treatment and those without response to treatment in serum M30 and M65 levels ( 572. 38 ± 349. 45 vs 436. 14 ± 285. 59 U / L, P = 0. 29; 817. 25± 307. 66 vs 703. 90 ± 221. 37 U / L, P = 0. 31) . Conclusion Serum levels of M30 and M65 are sensitive indicators for the necrosis and apoptosis of hepatocytes. Significant increases in serum M30 and M65 levels are observed in patients with HBV- related ACLF.

     

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