Correlation between expression of PI4KA in serum and HVB DNA load in patients with chronic hepatitis B

Objective To investigate the relationship between the expression of phosphatidylinositol 4- kinase III alpha ( PI4KA) in serum and HBV DNA load in patients with chronic hepatitis B ( CHB) . Methods Serum samples were collected from 180 patients with CHB, whose HBV DNA loads were determined by fluorescence quantitative PCR. According to the serum HBV DNA loads, the 180 patients were divided into three groups: high viral load group ( HBV DNA load > 107copies / ml) , medium viral load group ( 105copies / ml≤HBV DNA load≤107copies / ml) , and low viral load group ( HBV DNA load < 105copies / ml) , while the serum samples of 60 healthy people were selected as control group. Enzyme- linked immunosorbent assay was used to determine the concentration of PI4KA in peripheral serum. Results The concentrations of serum PI4KA were ( 2. 29 ± 0. 75) ng /ml in the control group, ( 2. 73 ± 0. 71) ng /ml in the low viral load group, ( 3. 52 ± 0. 78) ng / ml in the medium viral load group, and ( 4. 72 ± 0. 77) ng / ml in the high viral load group. There were significant differences in serum PI4KA concentration between the patient groups with different viral loads and control group ( F = 119. 958, P < 0. 01) , and the PI4KA levels in the three viral load groups were all significantly higher than those in the control group. The HBV DNA load and PI4KA concentration presented a positive correlation with each other ( r = 0. 758, P < 0. 01) - serum PI4KA level would be elevated with the increase in HBV DNA load. Conclusion The concentration of serum PI4KA is closely correlated with the HBV DNA load in patients with CHB, which suggests that PI4KA may play a crucial role in the HBV DNA replication.