Effects of Ruangan Huajian granules on mRNA expression of MMP- 13 and TIMP- 1 in liver tissue among C57 mice with hepatic fibrosis

Yang Li; Feng AiDong; Zheng HaoJie; Dai ErHei

doi:10.3969/j.issn.1001-5256.2014.04.013

Issue 4

Apr. 2014

Turn off MathJax

Article Contents

Abstract

References

Article Navigation > Journal of Clinical Hepatology > 2014 > 30(4): 340-343

PDF( 1780 KB)

Effects of Ruangan Huajian granules on mRNA expression of MMP- 13 and TIMP- 1 in liver tissue among C57 mice with hepatic fibrosis

DOI: 10.3969/j.issn.1001-5256.2014.04.013

Institute of Liver Diseases, The Fifth Hospital of Shijiazhuang

Research funding:

Published Date: 2014-04-20

Abstract

Abstract

Objective To observe the effects of Ruangan Huajian granules on the serum levels of matrix metalloproteinase-13 (MMP-13) and tissue inhibitor of metalloproteinase- 1 (TIMP- 1) and the mRNA expression of MMP- 13 and TIMP- 1 in liver tissue among C57 mice with hepatic fibrosis.Methods Forty C57 mice were randomly divided into normal control group (n = 10) , model control group (n = 10) , Ruangan Huajian granule group (n = 10) , and colchicine group (n = 10) .A rat model of hepatic fibrosis was established by intraperitoneal injection of carbon tetrachloride in the model control group, Ruangan Huajian granule group, and colchicine group.The serum levels of MMPs and TIMP- 1 and the mRNA expression of MMP- 13 and TIMP- 1 in liver tissue were measured by enzyme- linked immunosorbent assay and real- time quantitative PCR, respectively.Comparison of continuous data between groups was made by analysis of variance.Results Compared with the normal control group, the model control group had a significantly decreased serum level of MMPs (P = 0.003) and a significantly increased serum level of TIMP-1 (P = 0.027) .There were no significant differences in serum levels of MMPs and TIMP- 1 between the normal control group and Ruangan Huajian granule group (P = 0.364 and 0.217) .Compared with the normal control group, the Ruangan Huajian granule group had significantly increased mRNA expression of MMP- 13 in liver tissue (P = 0.005) , but the mRNA expression of TIMP- 1 showed no significant difference between the two groups (P = 0.997) .The model control group had significantly increased mRNA expression of TIMP- 1 in liver tissue compared with the normal control group (P = 0.009) .Conclusion Ruangan Huajian granules can not only promote the production of MMPs, but also inhibit the production of TIMP- 1, thus promoting extracellular matrix degradation, which may be one of the action mechanisms of Ruangan Huajian granules in the treatment of hepatic fibrosis.
- liver cirrhosis,
- Ruangan Huajian Particle,
- matrix metalloproteinases,
- tissue inhibitor of metalloproteinase-1,
- mice

FullText(HTML)

References(12)

References

[1]JIANG Y, AYITULA.Expression of TGFB1、TIMP1 in liver cirrhosis and pathological significance[J].J Clin Hepatol, 2009, 25 (1) :39-41. (in Chinese) 蒋莹, 阿依吐拉.转化生长因子-1和基质金属蛋白酶抑制因子-1在肝硬化组织中的表达及其临床意义[J].临床肝胆病杂志, 2009, 25 (1) :39-41.

[2]YE LH, WANG CK, YANG L, et al.Effects of Ruangan Huajian granule on hepatic expression ofα-SMA, TGFβ1, and COL-Ⅰin C57 mice with experimental hepatic fibrosis[J].J Clin Hepatol, 2012, 28 (9) :685-688. (in Chinese) 叶立红, 王翀奎, 杨莉, 等.软肝化坚颗粒对肝纤维化模型C57小鼠肝组织α-SMA、TGFβ1表达和Ⅰ型胶原水平的影响[J].临床肝胆病杂志, 2012, 28 (9) :685-688.

[3]YANG L, YE LH, TIAN YY, et al.Effect of ruanganhuajian keli on histopathology of hepatic fibrosis in mice[J].J Hebei Tradit Chin Med Pharmacol, 2011, 26 (1) :3-5. (in Chinese) 杨莉, 叶立红, 田岳飏, 等.软肝化坚颗粒对小鼠肝纤维化模型组织病理的影响[J].河北中医药学报, 2011, 26 (1) :3-5.

[4]SHUAI F.Effects of shosaikoto on MMPs and TIMPs mRNA inexperimental rat liver fibrosis[J].Anhui Med Pharm J, 2009, 13 (2) :139-141. (in Chinese) 帅峰.中药小柴胡汤对肝纤维化大鼠MMPs与TIMPs mRNA的影响[J].安徽医药, 2009, 13 (2) :139-141.

[5]CHEN KQ, ZHOU BY, CHEN YY, et al.Regulatory effeots of FXRactivationon expression of TIMP-1, TIMP-2 and MMP-2 in hepatic stellate cells[J].J Clin Hepatol, 2013, 29 (4) :290-293. (in Chinese) 陈科全, 周碧瑶, 陈雅莹, 等.法尼酯衍生物X受体活化对肝星状细胞TIMP-1、TIMP-2及MMP-2表达的调节作用[J].临床肝胆病杂志, 2013, 29 (4) :290-293.

[6]IIMURO Y, BRENNERDA.Matrix metalloproteinase gene delivery for liver fibrosis[J].Pharrn Res, 2008, 25 (2) :249-258.

[7]LI W, HUANG XH, SHEN ZQ, et al.Effect of sanqidanshen tablet on exper imental liver fibrosis induced by CCl4in mice[J].China Mod Doct, 2009, 47 (3) :42-44. (in Chinese) 李武, 黄学惠, 沈志强, 等.三七丹参对四氯化碳致小鼠实验性肝纤维化的影响[J].中国现代医生, 2009, 47 (3) :42-44.

[8]ZHENG HJ, HOU JL, LI JS, et al.Clinical observation the effect of Ruangan Huajian Particle on serological markers of liver fibrosis in patients with chronic hepatitis B[J].Hebei Med J, 2011, 33 (21) :3333-3334. (in Chinese) 郑浩杰, 侯军良, 李军生, 等.软肝化坚颗粒对慢性乙型肝炎患者肝纤维化血清标志物影响的临床观察[J].河北医药, 2011, 33 (21) :3333-3334.

[9]YANG L, LYU YH, LIU YZ, et al.Influence of Ruangan Huajian Particle on excretion of collagens and cytokines by hepatic stellate cells[J].Chin J Integr Tradit West Med Liver Dis, 2012, 22 (2) :104-106. (in Chinese) 杨莉, 吕宇航, 刘玉珍, 等.软肝化坚颗粒调节肝星状细胞分泌胶原及相关细胞因子的研究[J].中西医结合肝病杂志, 2012, 22 (2) :104-106.

[10]SENTHIL K, VENUGOPAL, JOY J, et al.Liver fibrosis causes downregulation of miRNA-150 and miRNA-194 in hepatic stellate cells, and their overexpression causes decreased stellate cell activationAm[J].J Physiol Gastrointest Liver Physiol, 2010, 298 (1) :g101-g106.

[11]ROBEN G, GIELING, KAREN W, et al.Interleukin-1 participates in the progression from liver injury to fibrosis[J].Am J Physiol Gastrointest Liver Physiol, 2009, 296 (6) :g1324-g1331.

[12]LIU TH, CONG M, WANG P, et al.Overexpression of MMP-13 inhibited collagen I expression in rat hepatic stellate cells[J].J Capit Med Univ, 2009, 30 (3) :313-316. (in Chinese) 刘天会, 丛敏, 王萍, 等.基质金属蛋白酶-13过表达抑制大鼠肝星状细胞中I型胶原表达[J].首都医科大学学报, 2009, 30 (3) :313-316.

Relative Articles

[1]	Rongjun LI, Chunxia XU, Ting ZHANG, Zhihong ZHANG, Lyu WANG. Role of salidroside in a mouse model of non-alcoholic fatty liver disease： A study based on nicotinamide phosphoribosyltransferase[J]. Journal of Clinical Hepatology, 2024, 40(1): 64-69. doi: 10.12449/JCH240112
[2]	Qian HUANG, Yan YANG, Rui ZENG, Menglin YAO, Qin SUN. Regulation of liver fibrosis by matrix metalloproteinase/tissue inhibitor of metalloproteinase and research advances in related therapeutic drugs[J]. Journal of Clinical Hepatology, 2022, 38(6): 1420-1425. doi: 10.3969/j.issn.1001-5256.2022.06.042
[3]	Yang ShangQing, Yang DongLiang, Liu Jia. Role of matrix metalloproteinases-2 and matrix metalloproteinases-9 in the development and progression of chronic liver diseases[J]. Journal of Clinical Hepatology, 2020, 36(10): 2364-2369. doi: 10.3969/j.issn.1001-5256.2020.10.045
[4]	Yang AiTing, Yan XuZhen, Zhao WenShan, Li WeiYu, Chen Wei, You Hong. Molecular mechanism of elastin and its regulatory factors on the formation and reversal of liver fibrosis in a mouse model of liver fibrosis[J]. Journal of Clinical Hepatology, 2020, 36(7): 1514-1519. doi: 10.3969/j.issn.1001-5256.2020.07.014
[5]	Zhang HongHai, Sun Yu, Liu Fang, Xie Li, Qiao LuXin, Chen DeXi, Yin JiMing. Determination and clinical significance of plasma matrix metalloproteinase in patients with hepatocellular carcinoma[J]. Journal of Clinical Hepatology, 2019, 35(4): 825-829. doi: 10.3969/j.issn.1001-5256.2019.04.023
[7]	Ge HongYan, Zhang ShiHua. Research advances in association between matrix metalloproteinases and liver fibrosis[J]. Journal of Clinical Hepatology, 2017, 33(3): 563-566. doi: 10.3969/j.issn.1001-5256.2017.03.037
[8]	Zhu HuiJia, Hou YiJun, Yuan KaiZhen, Xu LanMan, Lin Zhuo, Chen YongPing. Inhibitory effect of Anfate on expression of TGFβ1 /Smad2 signaling pathway in treatment of hepatic fibrosis in mice[J]. Journal of Clinical Hepatology, 2014, 30(4): 335-339. doi: 10.3969/j.issn.1001-5256.2014.04.012
[9]	Zhang SuMei, Xie YuMei, Hao ChunQiu, Kang WenZhen, Zhang Ying, Jiang Na, Ma Li, Peng MeiJuan, Jia ZhanSheng. Construction of the RNA interference vector of tissue inhibitor of matrix metalloproteinase -1 gene and its inhibitory effects[J]. Journal of Clinical Hepatology, 2012, 28(2): 135-138.
[10]	Wang YingChun, Liu YanFang, Zhu RuiPing, Song XinXin, Li XiaoHuan. Expression of histone deacetylase 1 and matrix metalloproteinase-2 in hepatocellular carcinoma and its significance[J]. Journal of Clinical Hepatology, 2012, 28(4): 276-278+288.
[11]	Ye LiHong, Wang ChongKui, Yang Li, Gao HuiXia, Zhao ZhaoXia, Hou JunLiang, Dai ErHei. Effects of Ruangan Huajian granule on hepatic expression of α-SMA, TGFβ1, and COL-Ⅰ in C57 mice with experimental hepatic fibrosis [J]. Journal of Clinical Hepatology, 2012, 28(9): 685-688.
[12]	Ye LiHong, Wang ChongKui, Tian YueYang, Xu Yi, Zheng HaoJie, Shao ShiXiang, Dai ErHei. Effect of Ruangan Huajian granule on hepatic expression of PDGF - B in C57 mice with experimental hepatic fibrosis[J]. Journal of Clinical Hepatology, 2011, 27(10): 1047-1050.
[13]	Jiang Ying, A YiTuLa. Expression of TGFβ1、TIMP1 in liver cirrhosis and pathological significance[J]. Journal of Clinical Hepatology, 2009, 25(1): 39-41.
[16]	Shao Ying. The expression of MMP-9 and TIMP-1 in HCC and clinical significance[J]. Journal of Clinical Hepatology, 2006, 22(1): 47-48.
[18]	Zhu ZhengYan, Du Zhi, Wang YiJun, Zhang Wen, Sun BaoCun. The Study on Relationship between E-cadherin, MMP-2 and MMP-9' expressions and the prognosis of hepatolellular carcinoma.[J]. Journal of Clinical Hepatology, 2002, 18(4): 235-236.

Supplements(0)

Cited By

Cited by

Periodical cited type(7)

1.	张旭，余嘉惠，王丽娜. 中医药防治慢性胰腺炎胰腺纤维化的机制研究. 中医学报. 2024(04): 758-764 .
2.	刘鑫，邱明亮，胡晓凤，彭婷，高月，张琦. 雷公藤甲素抗器官纤维化作用机制研究进展. 中草药. 2024(10): 3569-3578 .
3.	纪晓丹，龚彪，李兴佳，吕婵，徐莹. 中医药治疗胰腺纤维化的临床对策及研究进展. 临床肝胆病杂志. 2023(09): 2258-2264 . 本站查看
4.	李雪丽，刘钊. 雷公藤甲素通过调控AMPK/mTOR通路抑制脂肪细胞和巨噬细胞炎症机制研究. 辽宁中医药大学学报. 2022(12): 24-29+237 .
5.	戚潇禹，刘璐佳，杨阳，于海洋，王有鹏. 基于TGF-β_1/Smads信号通路中医药抗纤维化研究进展. 辽宁中医药大学学报. 2021(01): 58-62 .
6.	陈鑫，张永萍，徐剑，刘耀. 雷公藤甲素的现代研究进展. 贵州中医药大学学报. 2021(05): 85-89 .
7.	夏时海. 氧化苦参碱在防治胰腺纤维化的研究前景及临床应用价值. 世界华人消化杂志. 2020(17): 819-826 .

Other cited types(1)

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article Metrics

Article views (2588) PDF downloads(521)

Effects of Ruangan Huajian granules on mRNA expression of MMP- 13 and TIMP- 1 in liver tissue among C57 mice with hepatic fibrosis

DOI: 10.3969/j.issn.1001-5256.2014.04.013