Effects of Ruangan Huajian granules on mRNA expression of MMP- 13 and TIMP- 1 in liver tissue among C57 mice with hepatic fibrosis

Yang Li; Feng AiDong; Zheng HaoJie; Dai ErHei

doi:10.3969/j.issn.1001-5256.2014.04.013

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Apr. 2014

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Article Navigation > Journal of Clinical Hepatology > 2014 > 30(4): 340-343

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Effects of Ruangan Huajian granules on mRNA expression of MMP- 13 and TIMP- 1 in liver tissue among C57 mice with hepatic fibrosis

DOI: 10.3969/j.issn.1001-5256.2014.04.013

Institute of Liver Diseases, The Fifth Hospital of Shijiazhuang

Research funding:

Published Date: 2014-04-20

Abstract

Abstract

Objective To observe the effects of Ruangan Huajian granules on the serum levels of matrix metalloproteinase-13 (MMP-13) and tissue inhibitor of metalloproteinase- 1 (TIMP- 1) and the mRNA expression of MMP- 13 and TIMP- 1 in liver tissue among C57 mice with hepatic fibrosis.Methods Forty C57 mice were randomly divided into normal control group (n = 10) , model control group (n = 10) , Ruangan Huajian granule group (n = 10) , and colchicine group (n = 10) .A rat model of hepatic fibrosis was established by intraperitoneal injection of carbon tetrachloride in the model control group, Ruangan Huajian granule group, and colchicine group.The serum levels of MMPs and TIMP- 1 and the mRNA expression of MMP- 13 and TIMP- 1 in liver tissue were measured by enzyme- linked immunosorbent assay and real- time quantitative PCR, respectively.Comparison of continuous data between groups was made by analysis of variance.Results Compared with the normal control group, the model control group had a significantly decreased serum level of MMPs (P = 0.003) and a significantly increased serum level of TIMP-1 (P = 0.027) .There were no significant differences in serum levels of MMPs and TIMP- 1 between the normal control group and Ruangan Huajian granule group (P = 0.364 and 0.217) .Compared with the normal control group, the Ruangan Huajian granule group had significantly increased mRNA expression of MMP- 13 in liver tissue (P = 0.005) , but the mRNA expression of TIMP- 1 showed no significant difference between the two groups (P = 0.997) .The model control group had significantly increased mRNA expression of TIMP- 1 in liver tissue compared with the normal control group (P = 0.009) .Conclusion Ruangan Huajian granules can not only promote the production of MMPs, but also inhibit the production of TIMP- 1, thus promoting extracellular matrix degradation, which may be one of the action mechanisms of Ruangan Huajian granules in the treatment of hepatic fibrosis.
- liver cirrhosis,
- Ruangan Huajian Particle,
- matrix metalloproteinases,
- tissue inhibitor of metalloproteinase-1,
- mice

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References

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Effects of Ruangan Huajian granules on mRNA expression of MMP- 13 and TIMP- 1 in liver tissue among C57 mice with hepatic fibrosis

DOI: 10.3969/j.issn.1001-5256.2014.04.013