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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 8
Aug.  2016
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Article Contents

Effect of protein acetylation on hepatitis B virus replication

DOI: 10.3969/j.issn.1001-5256.2016.08.013
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  • Published Date: 2016-08-20
  • Objective To investigate the effect of protein acetylation in host cells on the replication of hepatitis B virus( HBV) in hepatocytes,since HBV infection greatly threatens human health and the acetylation of encoding proteins in infected cells plays an important role in HBV replication and infection. Methods The deacetylase inhibitors trichostatin A( TSA) and nicotinamide( NAM) were used to stimulate HBV replication in Hep G2. 2. 15 and Hep AD38 cells,and the HBV replication markers were measured. The pan- acetylysin protein and Ac- H3 were examined by Western Blot. Results The stimulation of cells with TSA and NAM increased the overall acetylation level of proteins in cells,and the acetylation level increased in a time- and dose- dependent manner. In the Hep G2. 2. 15 and Hep AD38 cells,stimulation with TSA and NAM reduced HBs Ag level in the supernatant of cell culture and increased HBV DNA level in a time- and dose- dependent manner,while HBe Ag in the supernatant of cell culture and DNA in cells did not change significantly. Conclusion Acetylation of host proteins may be involved in and affect HBV replication in cells,and further analysis and determination of host proteins whose acetylation affects HBV replication in cells help to learn more about the regulation of HBV replication and provide new thoughts for the development of specific antiviral strategies.

     

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