中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 6
Jun.  2017
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Article Contents

Changes in the function of dendritic cells after HCV loading mediated by CLEC4M

DOI: 10.3969/j.issn.1001-5256.2017.06.017
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  • Published Date: 2017-06-20
  • Objective To investigate the changes in the function of dendritic cells (DCs) after HCV adherence, possible mechanisms of HCV adherence of DCs mediated by CLEC4 M, and the impact of CLEC4 M on innate immune response mediated by DCs. Methods Peripheral blood mononuclear cells (PBMCs) were isolated from healthy people and induced to differentiate into mature DCs. Flow cytometry was used to measure the expression of CLEC4 M on the surface of DCs. Mature DCs were infected by HCVcc and positive control group, mannan interference group, CLEC4 M monoclonal antibody interference group, and blank control group were established. The supernatant was collected from each infected group, and ELISA was used to measure the changes in the levels of interleukin-10 (IL-10) and interleukin-12p70 (IL-12p70) in supernatant. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between any two groups. Results The positive rate of CLEC4 M in the surface of DCs reached 82. 6%. There were significant differences in the levels of IL-10 and IL-12p70 between the groups (F =54. 46 and 77. 72, both P < 0. 001) . The positive control group had significantly higher levels of IL-10 and IL-12p70 than the two interference groups (all P < 0. 001) , and compared with the blank control group, the positive control group, CLEC4 M monoclonal antibody interference group, and mannan interference group had significantly higher levels of IL-10 and IL-12p70 (all P < 0. 001) . Conclusion CLEC4 M mediates HCV adherence of DCs, participates in the body's immune response, and regulate the expression of DC immune molecules.

     

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