Effect of interference of Sema4D expression on cell biological characteristics in pancreatic cancer

Objective To investigate the effect of interference of Sema4D expression on cell biological behavior in pancreatic cancer.Methods Sema4D-siRNA was designed, synthesized, and transfected into a human pancreatic cancer cell line.The cells were divided into siRNA transfection group, negative control group (nonspecific transfection group) , and blank control group (untreated pancreatic cancer cells) .RT-q PCR was used to measure the change in the mRNA expression of Sema4D at 48 hours after transfection, and Western blot was used to measure the change in the protein expression of Sema4D at 72 hours after transfection.MTT assay was used to observe the change in cell growth after transfection, Transwell chamber invasion assay was used to observe the change in tumor cell invasion after transfection, and flow cytometry was used to observe the change in the apoptosis of tumor cells.An analysis of variance was used for comparison between multiple groups, and the least significant difference t-test was used for further comparison between any two groups.Results At 48 hours after Sema4D-siRNA transfection, the siRNA transfection group had significantly lower mRNA expression of Sema4D than the negative control group and the blank control group (F = 421.990, P < 0.001) .At 72 hours after Sema4D-siRNA transfection, the siRNA transfection group had significantly lower protein expression of Sema4D than the negative control group and the blank control group (F = 27.074, P =0.002 3) .At 48, 72, and 96 hours after transfection, the siRNA transfection group had a significantly lower cell growth rate than the negative control group and the blank control group (F = 15.314, 62.255, and 223.493, P = 0.004, < 0.001, and < 0.001) .The siRNA transfection group had a significantly lower number of cells which crossed the membrane than the negative control group and the blank control group (42.0 ± 5.9 vs 60.0 ± 6.1 vs 61.0 ± 4.6, F = 37.21, P = 0.000 4) .The siRNA transfection group had a significantly lower apoptosis rate than the negative control group and the blank control group [ (16.57 ± 0.31) % vs (9.50 ± 0.45) % vs (9.90 ± 0.61) %, F =26.75, P = 0.007 4].Conclusion Sema4D-siRNA can downregulate the expression of Sema4D in pancreatic cells and can inhibit the proliferation of pancreatic cancer cells and reduce their invasion ability and apoptosis.