Effect of amitriptyline on lipid deposition and biochemical metabolism in a cell model of nonalcoholic fatty liver disease

Qin LIU; Qiang ZHANG; Fangxiong WU; Rong YAN; Rong LI; Jia WANG; Chunyan NIU

doi:10.3969/j.issn.1001-5256.2021.01.020

Volume 37 Issue 1

Jan. 2021

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Effect of amitriptyline on lipid deposition and biochemical metabolism in a cell model of nonalcoholic fatty liver disease

DOI: 10.3969/j.issn.1001-5256.2021.01.020

1.
School of Medicine, Xiamen University, Xiamen, Fujian 361102, China
2.
Department of Gastroenterology, The First Affiliated Hospital of Xi′an Medical University, Xi′an 710077, China
3.
Nanjing Lishui People's Hospital; Department of Gastroenterology, Lishui Branch of Zhongda Hospital Affiliated to Southeast University, Nanjing 211200, China

Received Date: 2020-06-11
Accepted Date: 2020-08-28
Published Date: 2021-01-20

Abstract

Abstract

Objective To investigate the effect of amitriptyline on lipid deposition and biochemical metabolism in a cell model of nonalcoholic fatty liver disease (NAFLD) by regulating the acid sphingomyelinase (ASM)/ceramide (CE) pathway. Methods HepG2 and L02 cells were cultured in vitro to establish a cell model of NAFLD. MTT colorimetry was used to measure cell proliferation rate, and oil red O staining was used to observe the change of lipid droplets in cells. In the experiment, the cells were divided into normal control group, model group, Ami group, TNFα group, and Ami+TNFα group. An automatic biochemical analyzer was used to measure the levels of triglyceride (TG) and total cholesterol (TC) in cells and the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in supernatant; ELISA was used to measure the levels of CE and ASM in cells; Western blot was used to measure the protein expression ASM in cells, and RT-PCR was used to measure the mRNA expression of ASM in cells. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the Turkey test was used for further comparison between two groups. Results Compared with the normal control group, the NAFLD model group had significant increases in the protein and mRNA expression of ASM and the levels of CE, TG, TC, ALT, and AST (all P < 0.05). Compared with the model group, the Ami group had significant reductions in the protein and mRNA expression of ASM and the levels of CE, TG, TC, ALT, and AST (all P < 0.05), and the TNFα group had significant increases in the protein and mRNA expression of ASM and the levels of CE, TG, ALT, and AST (all P < 0.05). Compared with the TNFα group, the Ami+TNFα group had significant reductions in the protein and mRNA expression of ASM and the levels of CE, TG, TC, ALT, and AST (all P < 0.05). Conclusion The ASM/CE pathway promotes lipid accumulation and may lead to hepatocyte steatosis, and amitriptyline can alleviate lipid deposition in NAFLD hepatocytes by inhibiting the ASM/CE pathway.
- Non-alcoholic Fatty Liver Disease,
- Amitriptyline,
- PhospHolipases,
- Ceramides,
- Lipidoses

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Effect of amitriptyline on lipid deposition and biochemical metabolism in a cell model of nonalcoholic fatty liver disease

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Effect of amitriptyline on lipid deposition and biochemical metabolism in a cell model of nonalcoholic fatty liver disease

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