Composition and functional change of intestinal microbiota in infantile cholestasis

Jianli ZHOU; Zhaoxia WANG; Shaoming ZHOU; Qiao ZHANG; Xia QIAN

doi:10.3969/j.issn.1001-5256.2021.01.025

Volume 37 Issue 1

Jan. 2021

Turn off MathJax

Article Contents

Article Navigation > Journal of Clinical Hepatology > 2021 > 37(1): 126-130

PDF( 4055 KB)

Composition and functional change of intestinal microbiota in infantile cholestasis

DOI: 10.3969/j.issn.1001-5256.2021.01.025

Department of Gastroenterology, Shenzhen Children's Hospital, Shenzhen, Guangdong 518026, China

Received Date: 2020-07-05
Accepted Date: 2020-10-26
Published Date: 2021-01-20

Abstract

Abstract

Objective To investigate the composition and functional change of intestinal microbiota in infantile cholestasis (IC). Methods A total of 42 infants with IC who were admitted to Shenzhen Children's Hospital from September 2014 to February 2016 were enrolled as IC group, and 32 normal infants during the same period of time were enrolled as control group. Fecal samples were collected, and shotgun sequencing was used to investigate the features of intestinal microbiota. The Kyoto Encyclopedia of Genes and Genomes (KEGG) and eggNOG database were used to investigate the functional changes of intestinal microbiota. The Wilcoxon rank-sum test was used for comparison of continuous data between groups, and the chi-square test was used for comparison of categorical data between groups. Shannon coefficient, the Wilcoxon Rank sum test, and DESeq2 were used to calculate and analyze the data of strains and the functional pathways of genes, and Cytoscape was used to give a visual representation of abundant strains and their association. Results As for the etiology of IC in the IC group, 11(26.2%) had biliary atresia, 4(9.5%) had Citrin deficiency, 4(9.5%) had bile duct dysplasia, and 23(54.8%) had unknown cause. There was a significant difference in the composition of intestinal microbiota at the phylum level between the two groups (Z=0.000 046, P < 0.05), and the IC group had significant increases in Firmicutes and Bacteroidetes. There were also significant differences between the two groups at the species level (Z=0.000 014, P < 0.05), and the IC group had significant reductions in the probiotic bacteria including Bifidobacterium and significant increases in the potential pathogens including Veillonella and Niastella koreensis. There was a significant difference in the function of intestinal microbiota between the two groups (Z=0.019 8, P < 0.05), and the IC group had significant reductions in the functions of intestinal microbiota such as amino acid metabolism and carbohydrate metabolism. Conclusion Changes in the composition and function of intestinal microbiota are observed in infants with IC, with a significant reduction in probiotic bacteria and a significant increase in potential pathogens, and supplementation with probiotic bacteria including Bifidobacterium can reduce the production of potential pathogens and change disease progression.
- Infant,
- Cholestasis,
- Gastrointestinal Microbiome

FullText(HTML)

References(29)

References

[1]	LIU Y, HUANG ZH. Clinical diagnosis and treatment of cholestatic hepatopathy in infants[J]. J Clin Hepatol, 2015, 31(8): 1218-1220. (in Chinese) DOI: 10.3969/j.issn.1001-5256.2015.08.009 刘艳, 黄志华. 婴儿胆汁淤积性肝病的诊断与治疗[J]. 临床肝胆病杂志, 2015, 31(8): 1218-1220. DOI: 10.3969/j.issn.1001-5256.2015.08.009
[2]	FISCHLER B, LAMIREAU T. Cholestasis in the newborn and infant[J]. Clin Res Hepatol Gastroenterol, 2014, 38(3): 263-267. DOI: 10.1016/j.clinre.2014.03.010
[3]	CESARO C, TISO A, DEL PRETE A, et al. Gut microbiota and probiotics in chronic liver diseases[J]. Dig Liver Dis, 2011, 43(6): 431-438. DOI: 10.1016/j.dld.2010.10.015
[4]	TRAUNER M, FICKERT P, TILG H. Bile acids as modulators of gut microbiota linking dietary habits and inflammatory bowel disease: A potentially dangerous liaison[J]. Gastroenterology, 2013, 144(4): 844-846. DOI: 10.1053/j.gastro.2013.02.029
[5]	TRAUNER M, HALILBASIC E. Nuclear receptors as new perspective for the management of liver diseases[J]. Gastroenterology, 2011, 140(4): 1120-1125. e1-e12. DOI: 10.1053/j.gastro.2011.02.044
[6]	QIN N, YANG F, LI A, et al. Alterations of the human gut microbiome in liver cirrhosis[J]. Nature, 2014, 513(7516): 59-64. DOI: 10.1038/nature13568
[7]	ZHOU S, XU R, HE F, et al. Diversity of gut microbiota metabolic pathways in 10 pairs of Chinese infant twins[J]. PLoS One, 2016, 11(9): e0161627. DOI: 10.1371/journal.pone.0161627
[8]	HUSON DH, AUCH AF, QI J, et al. MEGAN analysis of metagenomic data[J]. Genome Res, 2007, 17(3): 377-386. DOI: 10.1101/gr.5969107
[9]	DIXON P. VEGAN, a package of R functions for community ecology[J]. J Veg Sci, 2003, 14(6): 927-930. DOI: 10.1111/j.1654-1103.2003.tb02228.x
[10]	MANYAM G, BIRERDINC A, BARANOVA A. KPP: KEGG pathway painter[J]. BMC Syst Biol, 2015, 9(Suppl 2): s3. DOI: 10.1186/1752-0509-9-S2-S3
[11]	JENSEN LJ, JULIEN P, KUHN M, et al. Eggnog: Automated construction and annotation of orthologous groups of genes[J]. Nucleic Acids Res, 2008, 36(Suppl 1): d250-d254. http://europepmc.org/abstract/MED/17942413
[12]	LOVE MI, HUBER W, ANDERS S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2[J]. Genome Biol, 2014, 15(12): 550. DOI: 10.1186/s13059-014-0550-8
[13]	SHANNON P, MARKIEL A, OZIER O, et al. Cytoscape: A software environment for integrated models of biomolecular interaction networks[J]. Genome Res, 2003, 13(11): 2498-2504. DOI: 10.1101/gr.1239303
[14]	HUANG Q, ZHANG HB, LI JT, et al. Research advances in the mechanism of action of intestinal microecology in intrahepatic cholestasis[J]. J Clin Hepatol, 2019, 35(10): 2355-2359. (in Chinese) DOI: 10.3969/j.issn.1001-5256.2019.10.050 黄倩, 张海博, 李京涛, 等. 肠道微生态在肝内胆汁淤积中的作用机制[J]. 临床肝胆病杂志, 2019, 35(10): 2355-2359. DOI: 10.3969/j.issn.1001-5256.2019.10.050
[15]	RIDLON JM, ALVES JM, HYLEMON PB, et al. Cirrhosis, bile acids and gut microbiota: Unraveling a complex relationship[J]. Gut Microbes, 2013, 4(5): 382-387. DOI: 10.4161/gmic.25723
[16]	COSTELLO EK, STAGAMAN K, DETHLEFSEN L, et al. The application of ecological theory toward an understanding of the human microbiome[J]. Science, 2012, 336(6086): 1255-1262. DOI: 10.1126/science.1224203
[17]	TROSVIK P, STENSETH NC, RUDI K. Convergent temporal dynamics of the human infant gut microbiota[J]. ISME J, 2010, 4(2): 151-158. DOI: 10.1038/ismej.2009.96
[18]	KOENIG JE, SPOR A, SCALFONE N, et al. Succession of microbial consortia in the developing infant gut microbiome[J]. Proc Natl Acad Sci U S A, 2011, 108 (Suppl 1): 4578-4585. http://emph.oxfordjournals.org/cgi/ijlink?linkType=ABST&journalCode=pnas&resid=108/Supplement_1/4578
[19]	OLSZAK T, AN D, ZEISSIG S, et al. Microbial exposure during early life has persistent effects on natural killer T cell function[J]. Science, 2012, 336(6080): 489-493. DOI: 10.1126/science.1219328
[20]	CHEN Y, YANG F, LU H, et al. Characterization of fecal microbial communities in patients with liver cirrhosis[J]. Hepatology, 2011, 54(2): 562-572. DOI: 10.1002/hep.24423
[21]	YANG L, GE WP, WANG R, et al. Influence of age, gender and delivery mode on the differential analysis of intestinal flora in infants[J]. Acta Nutrimenta Sinica, 2019, 41(4): 352-357. (in Chinese) https://www.cnki.com.cn/Article/CJFDTOTAL-YYXX201904015.htm 杨莉, 葛武鹏, 王瑞, 等. 不同月龄、性别、分娩方式对婴儿肠道菌群差异性分析[J]. 营养学报, 2019, 41(4): 352-357. https://www.cnki.com.cn/Article/CJFDTOTAL-YYXX201904015.htm
[22]	BÄCKHED F, ROSWALL J, PENG Y, et al. Dynamics and stabilization of the human gut microbiome during the first year of life[J]. Cell Host Microbe, 2015, 17(6): 852. DOI: 10.1016/j.chom.2015.05.012
[23]	BAJAJ JS, RIDLON JM, HYLEMON PB, et al. Linkage of gut microbiome with cognition in hepatic encephalopathy[J]. Am J Physiol Gastrointest Liver Physiol, 2012, 302(1): g168-g175. DOI: 10.1152/ajpgi.00190.2011
[24]	FUKUI H. Gut-liver axis in liver cirrhosis: How to manage leaky gut and endotoxemia[J]. World J Hepatol, 2015, 7(3): 425-442. DOI: 10.4254/wjh.v7.i3.425
[25]	BAUER TM, SCHWACHA H, STEINBRVCKNER B, et al. Small intestinal bacterial overgrowth in human cirrhosis is associated with systemic endotoxemia[J]. Am J Gastroenterol, 2002, 97(9): 2364-2370. DOI: 10.1111/j.1572-0241.2002.05791.x
[26]	WANG Y, GAO X, ZHANG X, et al. Gut microbiota dysbiosis is associated with altered bile acid metabolism in infantile cholestasis[J]. mSystems, 2019, 4(6): e00463-19. http://www.ncbi.nlm.nih.gov/pubmed/31848302
[27]	QIN J, LI Y, CAI Z, et al. A metagenome-wide association study of gut microbiota in type 2 diabetes[J]. Nature, 2012, 490(7418): 55-60. DOI: 10.1038/nature11450
[28]	GREENBLUM S, TURNBAUGH PJ, BORENSTEIN E. Metagenomic systems biology of the human gut microbiome reveals topological shifts associated with obesity and inflammatory bowel disease[J]. Proc Natl Acad Sci U S A, 2012, 109(2): 594-599. DOI: 10.1073/pnas.1116053109
[29]	LEPAGE P, HÄSLER R, SPEHLMANN ME, et al. Twin study indicates loss of interaction between microbiota and mucosa of patients with ulcerative colitis[J]. Gastroenterology, 2011, 141(1): 227-236. DOI: 10.1053/j.gastro.2011.04.011

Relative Articles

Supplements(0)

Cited By

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Figures(3) / Tables(1)

Get Citation

PDF

XML

Article Metrics

Article views (958) PDF downloads(45)

Composition and functional change of intestinal microbiota in infantile cholestasis

DOI: 10.3969/j.issn.1001-5256.2021.01.025

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Composition and functional change of intestinal microbiota in infantile cholestasis

DOI: 10.3969/j.issn.1001-5256.2021.01.025

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

About Journal

Submission Guideline

Journal Online

Hepatobiliary College

Guidelines

Export File

Citation

Format

Content