Effect of the expression level of diacylglycerol kinase gamma in paracancerous tissue on postoperative survival in patients with hepatocellular carcinoma

Xiangjun QIAN; Zhengyang GUO; Li WANG; Hanxiao NIE; Tao HE; Xiaoqian WANG; Baishun WAN; Mingjie YAO; Ling ZHANG

doi:10.3969/j.issn.1001-5256.2021.05.023

Volume 37 Issue 5

May 2021

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Article Navigation > Journal of Clinical Hepatology > 2021 > 37(5): 1091-1096

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Effect of the expression level of diacylglycerol kinase gamma in paracancerous tissue on postoperative survival in patients with hepatocellular carcinoma

DOI: 10.3969/j.issn.1001-5256.2021.05.023

1.
Department of Hepatopancreatobiliary Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China
2a.
Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China
2b.
Department of Anatomy and Histo-embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China
3.
Medical Research Center, Peking University Third Hospital, Beijing 100191, China

Received Date: 2020-10-02
Accepted Date: 2020-12-14
Published Date: 2021-05-20

Abstract

Abstract

Objective To investigate the effect of the expression level of diacylglycerol kinase gamma (DGKγ) in paracancerous tissue on the postoperative prognosis of patients with hepatocellular carcinoma (HCC) and its clinical value. Methods Related clinical data were collected from 78 HCC patients who were admitted and underwent surgical resection from December 2008 to August 2012 in the Affiliated Cancer Hospital of Zhengzhou University. Quantitative real-time PCR was used to measure the mRNA expression level of DGKγ in paracancerous tissue, and then the 78 patients were divided into low expression group (DGKγ < 0.086 2, LEP group) and high expression group (DGKγ ≥0.086 2, HEP group). Basic data and clinical features were compared between the two groups. The t-test and the Mann-Whitney U test were used for comparison of continuous data, and the chi-square test and the corrected chi-square test were used for comparison of categorical data. Univariate and multivariate Cox regression analyses were used to investigate independent influencing factors for survival and prognosis, and the Kaplan-Meier method was used to analyze the overall survival trends of all patients and the LEP and HEP groups in each subgroup of Barcelona Clinic Liver Cancer (BCLC) stages. Results The multivariate Cox regression analysis showed that the expression level of DGKγ (HR=1.913, 95%CI: 1.111-3.296, P=0.019), HBsAg (HR=2.645, 95%CI: 1.264- 5.537, P=0.010), Alb (HR=0.952, 95%CI: 0.916-0.990, P=0.013), BCLC stage (HR=1.702, 95%CI: 1.267-2.286, P < 0.001) and tumor size (HR=1.083, 95%CI: 1.019-1.152, P=0.011) were independent influencing factors for long-term survival of HCC patients; the LEP group had a significantly longer median survival time than the HEP group (45.0 months vs 22.9 months, P= 0.002 5). The stratified analysis showed that for BCLC stage A HCC, the LEP group had significantly better long-term survival than the HEP group (P=0.034 5); for BCLC stage B/C HCC, the LEP group had a longer median survival time than the HEP group (16.5 months vs 10.8 months), but there was no significant difference in short- and long-term survival between the two groups (P > 0.05). Conclusion The expression level of DGKγ in paracancerous tissue may be a new index for predicting and evaluating the long-term survival risk of HCC patients after surgery and has certain value in clinical application.
- Liver Neoplasms,
- Diglycerides,
- Prognosis

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[1]	BRAY F, FERLAY J, SOERJOMATARAM I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018, 68(6): 394-424. DOI: 10.3322/caac.21492.
[2]	TORRE LA, BRAY F, SIEGEL RL, et al. Global cancer statistics, 2012[J]. CA Cancer J Clin, 2015, 65(2): 87-108. DOI: 10.3322/caac.21262.
[3]	ZHOU M, WANG H, ZENG X, et al. Mortality, morbidity, and risk factors in China and its provinces, 1990-2017: A systematic analysis for the Global Burden of Disease Study 2017[J]. Lancet, 2019, 394(10204): 1145-1158. DOI: 10.1016/S0140-6736(19)30427-1.
[4]	LIU AX, WANG HQ, BO WT, et al. Clinical efficacy and prognostic factors analysis of hepatectomy for hepatocellular carcinoma[J]. Chin J Dig Surg, 2019, 18(4): 368-374. DOI: 10.3760/cma.j.issn.1673-9752.2019.04.012. 刘爱祥, 王海清, 薄文滔, 等. 肝细胞癌肝切除术的临床疗效及预后因素分析[J]. 中华消化外科杂志, 2019, 18(4): 368-374. DOI: 10.3760/cma.j.issn.1673-9752.2019.04.012.
[5]	European Association for the Study of the Liver. EASL Clinical Practice Guidelines: Management of hepatocellular carcinoma[J]. J Hepatol, 2018, 69(1): 182-236. DOI: 10.1016/j.jhep.2018.03.019.
[6]	Bureau of Medical Administration, National Health Commission of The People's Republic of China. Guidelines for diagnosis and treatment of primary liver cancer in China (2019 edition)[J]. J Clin Hepatol, 2020, 36(2): 277-292. DOI: 10.3969/j.issn.1001-5256.2020.02.007. 中华人民共和国国家卫生健康委员会医政医管局. 原发性肝癌诊疗规范(2019年版)[J]. 临床肝胆病杂志, 2020, 36(2): 277-292. DOI: 10.3969/j.issn.1001-5256.2020.02.007.
[7]	QIAN XJ, QU CF, LU FM. The tumor markers of hepatocellular carcinoma play an indispensable role in ultrasound screening and monitoring early hepatocellular carcinoma[J]. Liver, 2019, 24(8): 851-853. DOI: 10.3969/j.issn.1008-1704.2019.08.003. 钱相君, 曲春枫, 鲁凤民. 肝癌肿瘤标记物在超声筛查监测早期肝细胞癌中的作用不可或缺[J]. 肝脏, 2019, 24(8): 851-853. DOI: 10.3969/j.issn.1008-1704.2019.08.003.
[8]	HUANG C, FRETER C. Lipid metabolism, apoptosis and cancer therapy[J]. Int J Mol Sci, 2015, 16(1): 924-949. DOI: 10.3390/ijms16010924.
[9]	ARRANZ-NICOLÁS J, MÉRIDA I. Biological regulation of diacylglycerol kinases in normal and neoplastic tissues: New opportunities for cancer immunotherapy[J]. Adv Biol Regul, 2020, 75: 100663. DOI: 10.1016/j.jbior.2019.100663.
[10]	MÉRIDA I, TORRES-AYUSO P, ÁVILA-FLORES A, et al. Diacylglycerol kinases in cancer[J]. Adv Biol Regul, 2017, 63: 22-31. DOI: 10.1016/j.jbior.2016.09.005.
[11]	KAI M, YAMAMOTO E, SATO A, et al. Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer[J]. Mol Carcinog, 2017, 56(7): 1743-1752. DOI: 10.1002/mc.22631.
[12]	GUO Z, JIA J, YAO M, et al. Diacylglycerol kinase γ predicts prognosis and functions as a tumor suppressor by negatively regulating glucose transporter 1 in hepatocellular carcinoma[J]. Exp Cell Res, 2018, 373(1-2): 211-220. DOI: 10.1016/j.yexcr.2018.11.001.
[13]	HE Z, CHEN J, WANG J, et al. Expression of hepatitis B surface antigen in liver tissues can serve as a predictor of prognosis for hepatitis B virus-related hepatocellular carcinoma patients after liver resection[J]. Eur J Gastroenterol Hepatol, 2021, 33(1): 76-82. DOI: 10.1097/MEG.0000000000001698.
[14]	WANG L, LI Q, ZHANG J, et al. A novel prognostic scoring model based on albumin and γ-glutamyltransferase for hepatocellular carcinoma prognosis[J]. Cancer Manag Res, 2019, 11: 10685-10694. DOI: 10.2147/CMAR.S232073.
[15]	YAN X, YAO M, WEN X, et al. Elevated apolipoprotein B predicts poor postsurgery prognosis in patients with hepatocellular carcinoma[J]. Onco Targets Ther, 2019, 12: 1957-1964. DOI: 10.2147/OTT.S192631.
[16]	WU G, WU J, WANG B, et al. Importance of tumor size at diagnosis as a prognostic factor for hepatocellular carcinoma survival: A population-based study[J]. Cancer Manag Res, 2018, 10: 4401-4410. DOI: 10.2147/CMAR.S177663.
[17]	WU XF, LIU YW, ZHANG H, et al. Application value of different Barcelona clinical liver cancer Kinki staging in radical resection of liver cancer[J]. Chin J Dig Surg, 2020, 19(12): 1266-1272. DOI: 10.3760/cma.j.cn115610-20201102-00691. 吴晓峰, 刘一纬, 张慧, 等. 不同巴塞罗那临床肝癌Kinki分期在肝癌根治术中的应用价值[J]. 中华消化外科杂志, 2020, 19(12): 1266-1272. DOI: 10.3760/cma.j.cn115610-20201102-00691.
[18]	MASSART J, ZIERATH JR. Role of diacylglycerol kinases in glucose and energy homeostasis[J]. Trends Endocrinol Metab, 2019, 30(9): 603-617. DOI: 10.1016/j.tem.2019.06.003.
[19]	RAINERO E, CIANFLONE C, PORPORATO PE, et al. The diacylglycerol kinase α/atypical PKC/β1 integrin pathway in SDF-1α mammary carcinoma invasiveness[J]. PLoS One, 2014, 9(6): e97144. DOI: 10.1371/journal.pone.0097144.
[20]	FILIGHEDDU N, SAMPIETRO S, CHIANALE F, et al. Diacylglycerol kinase α mediates 17-β-estradiol-induced proliferation, motility, and anchorage-independent growth of Hec-1A endometrial cancer cell line through the G protein-coupled estrogen receptor GPR30[J]. Cell Signal, 2011, 23(12): 1988-1996. DOI: 10.1016/j.cellsig.2011.07.009.
[21]	DOMINGUEZ CL, FLOYD DH, XIAO A, et al. Diacylglycerol kinase α is a critical signaling node and novel therapeutic target in glioblastoma and other cancers[J]. Cancer Discov, 2013, 3(7): 782-797. DOI: 10.1158/2159-8290.CD-12-0215.
[22]	BACCHIOCCHI R, BALDANZI G, CARBONARI D, et al. Activation of alpha-diacylglycerol kinase is critical for the mitogenic properties of anaplastic lymphoma kinase[J]. Blood, 2005, 106(6): 2175-2182. DOI: 10.1182/blood-2005-01-0316.
[23]	KONG Y, ZHENG Y, JIA Y, et al. Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer[J]. Oncol Rep, 2016, 36(4): 1852-1860. DOI: 10.3892/or.2016.4989.
[24]	MCMURRAY HR, SAMPSON ER, COMPITELLO G, et al. Synergistic response to oncogenic mutations defines gene class critical to cancer phenotype[J]. Nature, 2008, 453(7198): 1112-1116. DOI: 10.1038/nature06973.
[25]	TAKEISHI K, TAKETOMI A, SHIRABE K, et al. Diacylglycerol kinase alpha enhances hepatocellular carcinoma progression by activation of Ras-Raf-MEK-ERK pathway[J]. J Hepatol, 2012, 57(1): 77-83. DOI: 10.1016/j.jhep.2012.02.026.

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Effect of the expression level of diacylglycerol kinase gamma in paracancerous tissue on postoperative survival in patients with hepatocellular carcinoma

DOI: 10.3969/j.issn.1001-5256.2021.05.023

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