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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 37 Issue 8
Aug.  2021
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Article Navigation >  Journal of Clinical Hepatology  > 2021  >  37(8): 1856-1860

Association of the expression of glucose-6-phosphate dehydrogenase with the prognosis of hepatocellular carcinoma

DOI: 10.3969/j.issn.1001-5256.2021.08.021

  • Department of Clinical Laboratory, Center for Gene Diagnosis, Program of Clinical Laboratory, Zhongnan Hospital of Wuhan University, Wuhan 430071, China

Research funding:

National Natural Science Foundation of China (81772276)

  • Received Date: 2020-12-29
  • Accepted Date: 2021-01-25
  • Published Date: 2021-08-20
    Abstract
  •   Objective  To investigate the expression of glucose-6-phosphate dehydrogenase (G6PD) in hepatocellular carcinoma (HCC) tissue and its association with prognosis.  Methods  HCC tissue samples and corresponding adjacent tissue samples were collected from 44 HCC patients who attended Zhongnan Hospital of Wuhan University from June 2016 to January 2018. RT-qRCR was used to measure the mRNA expression of G6PD, and the expression level of G6PD was compared between HCC tissue and adjacent tissue. According to the median of the mRNA expression of G6PD, HCC patients were divided into high G6PD expression group and low G6PD expression group and were analyzed with reference to the data in databases, and the Kaplan-Meier method was used to plot survival curves and investigate the differences in overall survival time and progression-free survival time between the two groups. The t-test was used for comparison of normally distributed continuous data between the two groups, and the Mann-Whitney U test was used for comparison of data with skewed distribution between the two groups; the Fisher's exact test was used for comparison of categorical data between the two groups; the Spearman method was used to perform a correlation analysis.  Results  The mRNA expression level of G6PD in HCC tissue was 2.09 times that in adjacent tissue (Z=-3.221, P=0.001). High expression of G6PD was a risk factor for overall survival time (hazard ratio [HR]=1.84, 95% confidence interval [CI: 1.30-2.61, P=0.000 52) and progression-free survival time (HR=1.75, 95%CI: 1.27-2.42, P=0.000 54) in HCC patients after hepatectomy. The low G6PD expression group had a significantly higher lymphocyte-monocyte ratio (LMR) than the high G6PD expression group (t=2.681, P=0.011), and G6PD was negatively correlated with LMR (r=-0.439, P=0.005).  Conclusion  Elevated expression of G6PD in HCC tissue may lead to a reduction in LMR in HCC patients, suggesting that G6PD expression might be associated with inflammation in tumor microenvironment. High expression of G6PD has a certain clinical value in evaluating the prognosis of HCC.

     

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