Current research status of hepatitis D virus RNA detection

Yao GAO; Feng REN; Zhongping DUAN

doi:10.3969/j.issn.1001-5256.2023.04.004

Volume 39 Issue 4

Apr. 2023

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Article Navigation > Journal of Clinical Hepatology > 2023 > 39(4): 758-765

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Current research status of hepatitis D virus RNA detection

DOI: 10.3969/j.issn.1001-5256.2023.04.004

Yao GAO^{1, 2},
Feng REN^{1, 2},
Zhongping DUAN^{1
,
,
,}

1.
Beijing Municipal Key Laboratory of Liver Failure and Artificial Liver Treatment Research, Beijing YouAn Hospital, Capital Medical University, Beijing 100069, China
2.
Beijing Institute of Hepatology, Beijing 100069, China

Research funding:

National Natural Science Foundation of China (81770611);

National Natural Science Foundation of China (82002243);

Key Projects of the Beijing Municipal Education Commission's Science and Technology Plan (KZ202010025035);

Special Key Research Project of Capital Health Development Scientific Research (SF2020-1-1151);

Pilot Project of Public Welfare Development and Reform of Beijing Municipal Medical Research Institutes (Beijing Medical Research No. 2021-10);

High-level Public Health Technical Personnel Construction Project (Subject leaders-02-13);

Talent Cultivation plan of "Climbing the peak" of Beijing Municipal Hospital Administration (DFL20221503);

Research and Cultivation Foundation of Capital Medical University (PYZ22130)

More Information

Corresponding author: DUAN Zhongping, duan2517@163.com (ORCID: 0000-0002-8455-0426)
Received Date: 2023-02-21
Accepted Date: 2023-03-21
Published Date: 2023-04-20

Abstract

Abstract

Hepatitis D caused by hepatitis D virus (HDV) infection is the most serious type of viral hepatitis. The prevalence rate of HDV has been seriously underestimated due to the lack of accurate HDV RNA detection methods. HDV RNA is the gold standard for the diagnosis of HDV infection and is of great significance in the diagnosis, screening, and treatment guidance of HDV. However, the multiple genotypes and strong secondary structure of HDV have led to great difficulties in HDV RNA detection. This article reviews the advances in HDV RNA detection methods and elaborates on the development from qualitative to quantitative detection methods, in order to provide new ideas for understanding the significance of HDV RNA detection and promoting the research and development of new HDV RNA detection methods.
- Hepatitis Delta Virus,
- RNA,
- Diagnosis

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References(45)

References

[1]	STOCKDALE AJ, KREUELS B, HENRION M, et al. The global prevalence of hepatitis D virus infection: Systematic review and meta-analysis[J]. J Hepatol, 2020, 73(3): 523-532. DOI: 10.1016/j.jhep.2020.04.008.
[2]	RIZZETTO M, CANESE MG, ARICÒ S, et al. Immunofluorescence detection of new antigen-antibody system (delta/anti-delta) associated to hepatitis B virus in liver and in serum of HBsAg carriers[J]. Gut, 1977, 18(12): 997-1003. DOI: 10.1136/gut.18.12.997.
[3]	CHEN S, REN F, HUANG X, et al. Underestimated prevalence of HIV, hepatitis B virus (HBV), and hepatitis D virus (HDV) triple infection globally: systematic review and meta-analysis[J]. JMIR Public Health Surveill, 2022, 8(11): e37016. DOI: 10.2196/37016.
[4]	MIAO Z, ZHANG S, OU X, et al. Estimating the global prevalence, disease progression, and clinical outcome of hepatitis delta virus infection[J]. J Infect Dis, 2020, 221(10): 1677-1687. DOI: 10.1093/infdis/jiz633.
[5]	European Association for the Study of the Liver. EASL 2017 Clinical Practice Guidelines on the management of hepatitis B virus infection[J]. J Hepatol, 2017, 67(2): 370-398. DOI: 10.1016/j.jhep.2017.03.021.
[6]	SARIN SK, KUMAR M, LAU GK, et al. Asian-Pacific clinical practice guidelines on the management of hepatitis B: a 2015 update[J]. Hepatol Int, 2016, 10(1): 1-98. DOI: 10.1007/s12072-015-9675-4.
[7]	TERRAULT NA, LOK A, MCMAHON BJ, et al. Update on prevention, diagnosis, and treatment of chronic hepatitis B: AASLD 2018 hepatitis B guidance[J]. Hepatology, 2018, 67(4): 1560-1599. DOI: 10.1002/hep.29800.
[8]	WHO Guidelines Approved by the Guidelines Review Committee. Guidelines for the prevention, care and treatment of persons with chronic hepatitis B infection[R]. Geneva: World Health Organization, 2015.
[9]	WONG SK, LAZINSKI DW. Replicating hepatitis delta virus RNA is edited in the nucleus by the small form of ADAR1[J]. Proc Natl Acad Sci U S A, 2002, 99(23): 15118-15123. DOI: 10.1073/pnas.232416799.
[10]	LI J, WANDS J. Hepatitis B and D viral receptors[J]. Hepatology, 2016, 63(1): 11-13. DOI: 10.1002/hep.28131.
[11]	GIERSCH K, BHADRA OD, VOLZ T, et al. Hepatitis delta virus persists during liver regeneration and is amplified through cell division both in vitro and in vivo[J]. Gut, 2019, 68(1): 150-157. DOI: 10.1136/gutjnl-2017-314713.
[12]	DÉNY P. Hepatitis delta virus genetic variability: from genotypes Ⅰ, Ⅱ, Ⅲ to eight major clades?[J]. Curr Top Microbiol Immunol, 2006, 307: 151-171. DOI: 10.1007/3-540-29802-9_8.
[13]	CAREDDA F, ROSSI E, D'ARMINIO MONFORTE A, et al. Hepatitis B virus-associated coinfection and superinfection with delta agent: indistinguishable disease with different outcome[J]. J Infect Dis, 1985, 151(5): 925-928. DOI: 10.1093/infdis/151.5.925.
[14]	ROMEO R, FOGLIENI B, CASAZZA G, et al. High serum levels of HDV RNA are predictors of cirrhosis and liver cancer in patients with chronic hepatitis delta[J]. PLoS One, 2014, 9(3): e92062. DOI: 10.1371/journal.pone.0092062.
[15]	WRANKE A, WEDEMEYER H. Antiviral therapy of hepatitis delta virus infection - progress and challenges towards cure[J]. Curr Opin Virol, 2016, 20: 112-118. DOI: 10.1016/j.coviro.2016.10.002.
[16]	TAN YC, LEE GH, HUANG DQ, et al. Future anti-HDV treatment strategies, including those aimed at HBV functional cure[J]. Liver Int, 2022. DOI: 10.1111/liv.15387.[Online ahead of print]
[17]	KOH C, HELLER T, GLENN JS. Pathogenesis of and new therapies for hepatitis D[J]. Gastroenterology, 2019, 156(2): 461-476. DOI: 10.1053/j.gastro.2018.09.058.
[18]	ZIGNEGO AL, DENY P, FERAY C, et al. Amplification of hepatitis delta virus RNA sequences by polymerase chain reaction: a tool for viral detection and cloning[J]. Mol Cell Probes, 1990, 4(1): 43-51. DOI: 10.1016/0890-8508(90)90038-2.
[19]	MADEJÓN A, CASTILLO I, BARTOLOMÉ J, et al. Detection of HDV-RNA by PCR in serum of patients with chronic HDV infection[J]. J Hepatol, 1990, 11(3): 381-384. DOI: 10.1016/0168-8278(90)90225-g.
[20]	MADEJÓN A, CASTILLO I, COTONAT T, et al. Significance of low HDV replication levels during the natural history of HDV infection. Long-term follow-up[J]. J Hepatol, 1993, 17(Suppl 3): S157-S160. DOI: 10.1016/s0168-8278(05)80443-x.
[21]	YAMASHIRO T, NAGAYAMA K, ENOMOTO N, et al. Quantitation of the level of hepatitis delta virus RNA in serum, by real-time polymerase chain reaction—and its possible correlation with the clinical stage of liver disease[J]. J Infect Dis, 2004, 189(7): 1151-1157. DOI: 10.1086/382133.
[22]	LE GAL F, GORDIEN E, AFFOLABI D, et al. Quantification of hepatitis delta virus RNA in serum by consensus real-time PCR indicates different patterns of virological response to interferon therapy in chronically infected patients[J]. J Clin Microbiol, 2005, 43(5): 2363-2369. DOI: 10.1128/JCM.43.5.2363-2369.2005.
[23]	KIM HS, KIM SJ, PARK HW, et al. Prevalence and clinical significance of hepatitis D virus co-infection in patients with chronic hepatitis B in Korea[J]. J Med Virol, 2011, 83(7): 1172-1177. DOI: 10.1002/jmv.22095.
[24]	MAKHLOUF NA, MORSY KH, MAHMOUD AA. Hepatitis D virus infection among hepatitis B virus surface antigen positive individuals in Upper Egypt: Prevalence and clinical features[J]. J Infect Public Health, 2019, 12(3): 350-356. DOI: 10.1016/j.jiph.2018.12.001.
[25]	MOATTER T, ABBAS Z, SHABIR S, et al. Clinical presentation and genotype of hepatitis delta in Karachi[J]. World J Gastroenterol, 2007, 13(18): 2604-2607. DOI: 10.3748/wjg.v13.i18.2604.
[26]	SCARPONI CF, KROON EG, VIEIRA DS, et al. Molecular epidemiology of Hepatitis delta virus infection in Minas Gerais state from Brazil, an area outside the hyperendemic region of the Amazon Basin[J]. Mem Inst Oswaldo Cruz, 2019, 114: e190074. DOI: 10.1590/0074-02760190074.
[27]	JACHS M, BINTER T, SCHMIDBAUER C, et al. Hepatitis D virus (HDV) prevalence in Austria is low but causes considerable morbidity due to fast progression to cirrhosis[J]. United European Gastroenterol J, 2021, 9(10): 1119-1127. DOI: 10.1002/ueg2.12163.
[28]	RAMIA S, EL-ZAATARI M, SHARARA AI, et al. Current prevalence of hepatitis delta virus (HDV) infection and the range of HDV genotypes in Lebanon[J]. Epidemiol Infect, 2007, 135(6): 959-962. DOI: 10.1017/S0950268806007643.
[29]	MELO DA SILVA E, KAY A, LOBATO C, et al. Non-F HBV/HDV-3 coinfection is associated with severe liver disease in Western Brazilian Amazon[J]. J Med Virol, 2019, 91(6): 1081-1086. DOI: 10.1002/jmv.25411.
[30]	CASTELNAU C, LE GAL F, RIPAULT MP, et al. Efficacy of peginterferon alpha-2b in chronic hepatitis delta: relevance of quantitative RT-PCR for follow-up[J]. Hepatology, 2006, 44(3): 728-735. DOI: 10.1002/hep.21325.
[31]	WOLTERS LM, VAN NUNEN AB, HONKOOP P, et al. Lamivudine-high dose interferon combination therapy for chronic hepatitis B patients co-infected with the hepatitis D virus[J]. J Viral Hepat, 2000, 7(6): 428-434. DOI: 10.1046/j.1365-2893.2000.00254.x.
[32]	BOTELHO-SOUZA LF, SOUZA VIEIRA D, DE OLIVEIRA DOS SANTOS A, et al. Characterization of the genotypic profile of hepatitis delta virus: Isolation of HDV genotype-1 in the western Amazon region of Brazil[J]. Intervirology, 2015, 58(3): 166-171. DOI: 10.1159/000431040.
[33]	CELIK I, KARATAYLI E, CEVIK E, et al. Complete genome sequences and phylogenetic analysis of hepatitis delta viruses isolated from nine Turkish patients[J]. Arch Virol, 2011, 156(12): 2215-2220. DOI: 10.1007/s00705-011-1120-y.
[34]	KARATAYL₁ E, ALTUNOǦLU YÇ, KARATAYL₁ SC, et al. A one step real time PCR method for the quantification of hepatitis delta virus RNA using an external armored RNA standard and intrinsic internal control[J]. J Clin Virol, 2014, 60(1): 11-15. DOI: 10.1016/j.jcv.2014.01.021.
[35]	KATSOULIDOU A, MANESIS E, ROKKA C, et al. Development and assessment of a novel real-time PCR assay for quantitation of hepatitis D virus RNA to study viral kinetics in chronic hepatitis D[J]. J Viral Hepat, 2013, 20(4): 256-262. DOI: 10.1111/jvh.12000.
[36]	SELVEY S, THOMPSON EW, MATTHAEI K, et al. Beta-actin—an unsuitable internal control for RT-PCR[J]. Mol Cell Probes, 2001, 15(5): 307-311. DOI: 10.1006/mcpr.2001.0376.
[37]	DHANASEKARAN S, DOHERTY TM, KENNETH J, et al. Comparison of different standards for real-time PCR-based absolute quantification[J]. J Immunol Methods, 2010, 354(1-2): 34-39. DOI: 10.1016/j.jim.2010.01.004.
[38]	LE GAL F, BRICHLER S, SAHLI R, et al. First international external quality assessment for hepatitis delta virus RNA quantification in plasma[J]. Hepatology, 2016, 64(5): 1483-1494. DOI: 10.1002/hep.28772.
[39]	WANG C, SHEN X, LU J, et al. Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) system for rapid detection of HDV genotype 1[J]. Lett Appl Microbiol, 2013, 56(3): 229-235. DOI: 10.1111/lam.12039.
[40]	KOJABAD AA, FARZANEHPOUR M, GALEH H, et al. Droplet digital PCR of viral DNA/RNA, current progress, challenges, and future perspectives[J]. J Med Virol, 2021, 93(7): 4182-4197. DOI: 10.1002/jmv.26846.
[41]	LIU X, FENG J, ZHANG Q, et al. Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets[J]. Emerg Microbes Infect, 2020, 9(1): 1175-1179. DOI: 10.1080/22221751.2020.1772679.
[42]	CAVIGLIA GP, ABATE ML, TANDOIF, et al. Quantitation of HBV cccDNA in anti-HBc-positive liver donors by droplet digital PCR: A new tool to detect occult infection[J]. J Hepatol, 2018, 69(2): 301-307. DOI: 10.1016/j.jhep.2018.03.021.
[43]	CASSIDY N, FISH CS, LEVY CN, et al. HIV reservoir quantification using cross-subtype multiplex ddPCR[J]. iScience, 2022, 25(1): 103615. DOI: 10.1016/j.isci.2021.103615.
[44]	OLIVERO A, ROSSO C, CIANCIO A, et al. Clinical application of droplet digital PCR for hepatitis delta virus quantification[J]. Biomedicines, 2022, 10(4): 792. DOI: 10.3390/biomedicines10040792.
[45]	XU L, ZHANG X, CAO Y, et al. Digital droplet PCR for detection and quantitation of hepatitis delta virus[J]. Clin Transl Gastroenterol, 2022, 13(7): e00509. DOI: 10.14309/ctg.0000000000000509.

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Current research status of hepatitis D virus RNA detection

DOI: 10.3969/j.issn.1001-5256.2023.04.004

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