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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 39 Issue 4
Apr.  2023
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Article Navigation >  Journal of Clinical Hepatology  > 2023  >  39(4): 986-992

Advances in in vitro induction of hepatocyte-like cells and the application of small-molecule compounds in inducing hepatocyte-like cells

DOI: 10.3969/j.issn.1001-5256.2023.04.037

  • Fujian Key Laboratory of Development and Neurobiology, College of Life Sciences, Fujian Normal University, Fuzhou 350117, China

Research funding:

Natural Science Foundation of Fujian Province (2020J05038);

Fujian Education Department (JAT190090)

More Information
  • Corresponding author: TANG Wei, 40720007@qq.com (ORCID: 0000-0001-8841-4125)
  • Received Date: 2022-09-05
  • Accepted Date: 2022-10-12
  • Published Date: 2023-04-20
    Abstract
  • The induction of hepatocyte-like cells (HLCs) in vitro is one of the effective ways to obtain a large number of useful hepatocyte, and these HLCs can be used in disease modeling, drug design, and toxicological evaluation. At present, the induction of HLCs in vitro is mainly achieved by introducing exogenous transcription factors, cytokines or small-molecule compounds. Since small-molecule compounds have the advantages of structural diversity, controllable time and dose, and convenient and safe operation, scientists are devoted to screening out the small-molecule compounds to replace exogenous transcription factors and cytokines, and such compounds have a promising application prospect in the field of regenerative medicine. This article reviews the studies on the in vitro induction of HLCs from pluripotent stem cells and other adult stem cells and summarizes the application of small-molecule compounds in the in vitro induction of HLCs, in order to provide ideas and references for the in vitro induction of HLCs.

     

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