胆管阻塞性大鼠肝纤维化模型肝组织MMP2m RNA的表达及复方861抑制作用的研究

To study the effect of Cpd 861 on MMP2mRNA expression in liver fibrosis induced by bile duct occlusion in rats. The bile ducts of female Wistar rats were completely occluded by retrograde injection of Histoacryl and ligation.Groups of the rats were treated with Cpd 861 3g/kg?6g/kg?9g/kg after operation of seven days.The rats were sacrificed and MMP2mRNA were detected by semi-quantitative RT-PCR at 49 days after treatment.MMP2mRNA levels of model group (0 633±0 350) was highter than that of control group (0 025±0 018) , P <0 01.Treartment groups of Cpd 861 were lower (0 107±0 070?0 248±0 0192?0 222±0 106) than that of model group ( P <0 05) . MMP2mRNA levels are remarkably increased in fibrotic bile duct occlusion madel group.The antifibrotic mechanism of Cpd 861 was partly due to its down-regulation on MMP2mRNA levels.