武汉地区散发性戊型肝炎流行病学及病毒基因型

To investigate the epidemiology and the genotypes of hepatitis E virus existed in Wuhan.363 serum samples were collected from patients with hepatitis E in Tongji Hospital of Wuhan.The partial genome of open reading frame 2 of 120 HEV was amplified using polymerase chain reaction, 25 of which were sequenced.Clustlx and Treeview softwares were used for nucleotide sequences phylogenetic analysis of HEV among genotype Ⅰ, Ⅱ, Ⅲ, Ⅳ and HEV of Wuhan.And enrolled the 916 cases clinically diagnosed as acute hepatitis E during the period of January 2000 to August 2004.The results showed 25 isolates shared the same genotype with nucleotides of 82.61-98.55%.They had 76.52-81.74%, 70.43-73.04%, 76.52-81.16%, and 84.35-88.70% homology at the nucleotide level with HEV genotypes 1-4, respectively.Phylogenetic analysis suggested that these 25 isolates maybe represent 3 different subtypes at least.HEV sequences isolated from patients of Wuhan belong to HEV genotype 4 with different subtypes.The infection rate of HEV is increasing now.Male patients are 3.3 times high than women patients in clinic investigation.Age from 30 to 59 and time from March to June are susceptible factors for patients to take hepatitis E.