乙型肝炎病毒前C区和BCP区突变及基因型对HBeAg表达的影响

To study the affection of the mutations and genotypes of hepatitis B virus precore and basic core promoter (BCP) on HBeAg expression.Gene chip technique was applied to detect gene mutations in HBV pre-core nt.1896, BCP nt.1762 and 1764.And DNA sequencing technique was used to sequence their genotypes Time-resolution immunofluorescenece technique was used to quantitate HBeAg.Those samples were divided according to the conditions mutations and genotypes of precore and BCP. The quantity of HBeAg were significant difference between single 1762/1764 mutation group, single 1896 mutation group, 1896/1762/1764 unite mutations group and no above mutations group (F=6.47, P<0.01) .There was no significant different between genotype B group and genotype C group in quantity of HBeAg (t=0.1394, P>0.05) .Hepatitis B virus precore and BCP mutations might significantly affect the expression of HBeAg, and lead to the variety of HBV pathogenicity.