实时荧光定量PCR分析慢性丙型肝炎患者胸腺近期输出功能

Objective To analyze the content of T-cell receptor excision circles (TRECs) within peripheral blood mononuclear cells of chronic hepatitis C (CHC) patients to value the thymic output function of CHC and investigate the relation of thymic output function and HCV RNA in chronic hepatitis C (CHC) patients.Methods Quantitative detection of TREcs in DNA of peripheral blood mononuclear cells (PBMCs) from 22cases with CHC, 16 normal individuals was performed by real-time fluorescence quantitative PCR, and detection of HCV RNA of peripheral blood serum in CHC by RT-PCR with fluorescent probe technology.Results The mean of TRECs in patients with CHC (5.52±1.87 copies/103PBMCs) was lower than that in normal individuals (7.19±2.42 copies/103 PBMCs, P<0.05) ;The value of TRECs in patients with CHC was inversely correlated with HCV RNA (γ=-0.427, P<0.05) .Conclusion TRECs levels in CHC are lower in the healthy and inversely correlated with HCV RNA.